Invitrogen

CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine7, eBioscience™

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CD69 Antibody (25-0691-82) in Flow

Staining of ConA-stimulated splenocytes with Anti-Mouse CD69 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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产品信息

25-0691-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看77篇已发表文章 77篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

H1.2F3

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_469637

产品详细信息

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

Applications Reported: The H1.2F3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

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MAIT cells contribute to protection against lethal influenza infection in vivo.

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Homoharringtonine induced immune alteration for an Efficient Anti-tumor Response in Mouse Models of Non-small Cell Lung Adenocarcinoma Expressing Kras Mutation.

Scientific reports

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Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

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Scientific reports

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Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

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Differentiation and Protective Capacity of Virus-Specific CD8⁺ T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

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Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling.

The Journal of clinical investigation

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Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

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The Journal of experimental medicine

Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D

25-0691 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice.

Mon May 01 00:00:00 EDT 2017

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Intestinal helminth infection induces highly functional resident memory CD4⁺ T cells in mice.

European journal of immunology

Steinfelder S,Rausch S,Michael D,Kühl AA,Hartmann S

Wed Feb 01 00:00:00 EST 2017

14-3-3 Proteins regulate K_2P 5.1 surface expression on T lymphocytes.

Traffic (Copenhagen, Denmark)

Fernández-Orth J,Ehling P,Ruck T,Pankratz S,Hofmann MS,Landgraf P,Dieterich DC,Smalla KH,Kähne T,Seebohm G,Budde T,Wiendl H,Bittner S,Meuth SG

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Reactive Oxygen Species Regulate the Inflammatory Function of NKT Cells through Promyelocytic Leukemia Zinc Finger.

Journal of immunology (Baltimore, Md. : 1950)

Kim YH,Kumar A,Chang CH,Pyaram K

25-0691 was used in Flow cytometry/Cell sorting to investigate the role of reactive oxygen species in natural killer T cell function.

Wed Nov 15 00:00:00 EST 2017

IRF6 Regulates Alternative Activation by Suppressing PPARγ in Male Murine Macrophages.

Endocrinology

Li C,Ying W,Huang Z,Brehm T,Morin A,Vella AT,Zhou B

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

IL-27R signaling controls myeloid cells accumulation and antigen-presentation in atherosclerosis.

Scientific reports

Peshkova IO,Fatkhullina AR,Mikulski Z,Ley K,Koltsova EK

25-0691 was used in Flow cytometry/Cell sorting to study the role of IL-27R in controlling myeloid cell accumulation and activation in atherosclerosis.

Tue May 23 00:00:00 EDT 2017

NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20.

The Journal of experimental medicine

Drennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D

25-0691 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells.

Mon Sep 19 00:00:00 EDT 2016

Dendritic cell sphingosine-1-phosphate lyase regulates thymic egress.

The Journal of experimental medicine

Zamora-Pineda J,Kumar A,Suh JH,Zhang M,Saba JD

25-0691 was used in Flow cytometry/Cell sorting to investigate the role of dendritic cell sphingosine-1-phosphate lyase in thymic egress.

Mon Nov 14 00:00:00 EST 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

MicroRNA-223 is a crucial mediator of PPARγ-regulated alternative macrophage activation.

The Journal of clinical investigation

Ying W,Tseng A,Chang RC,Morin A,Brehm T,Triff K,Nair V,Zhuang G,Song H,Kanameni S,Wang H,Golding MC,Bazer FW,Chapkin RS,Safe S,Zhou B

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

MFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation.

Stem cell reports

Tan Y,AlKhamees B,Jia D,Li L,Couture JF,Figeys D,Jinushi M,Wang L

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication.

Oncoimmunology

Draghiciu O,Nijman HW,Hoogeboom BN,Meijerhof T,Daemen T

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Natural killer cell NKG2D and granzyme B are critical for allergic pulmonary inflammation.

The Journal of allergy and clinical immunology

Farhadi N,Lambert L,Triulzi C,Openshaw PJ,Guerra N,Culley FJ

25-0691 was used in Flow cytometry/Cell sorting to investigate the importance of natural killer cells in house dust mite-triggered allergic response.

Sat Mar 01 00:00:00 EST 2014

mTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function.

Journal of immunology (Baltimore, Md. : 1950)

Donnelly RP,Loftus RM,Keating SE,Liou KT,Biron CA,Gardiner CM,Finlay DK

25-0691 was used in Flow cytometry/Cell sorting to investigate whether mTORC1-controlled metabolism underpins normal natural killer cell proinflammatory functions.

Sat Nov 01 00:00:00 EDT 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

Published figure using CD69 monoclonal antibody (Product # 25-0691-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2015

Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation.

Nature communications

Fu H,Kishore M,Gittens B,Wang G,Coe D,Komarowska I,Infante E,Ridley AJ,Cooper D,Perretti M,Marelli-Berg FM

25-0691 was used in Flow cytometry/Cell sorting to explain the mechanisms underlying the powerful migratory capacity of Treg cells and their ability to maintain optimal T cell ratios.

Fri Mar 14 00:00:00 EDT 2014

Sequential transcriptional changes dictate safe and effective antigen-specific immunotherapy.

Nature communications

Burton BR,Britton GJ,Fang H,Verhagen J,Smithers B,Sabatos-Peyton CA,Carney LJ,Gough J,Strobel S,Wraith DC

25-0691 was used in Flow cytometry/Cell sorting to study the markers significant for antigen-specific immunotherapy such as regulatory CD4(+) T-cell transcription factors.

Wed Sep 03 00:00:00 EDT 2014

Plasmacytoid dendritic cells play a key role in promoting atherosclerosis in apolipoprotein E-deficient mice.

Arteriosclerosis, thrombosis, and vascular biology

Macritchie N,Grassia G,Sabir SR,Maddaluno M,Welsh P,Sattar N,Ialenti A,Kurowska-Stolarska M,McInnes IB,Brewer JM,Garside P,Maffia P

25-0691 was used in Flow cytometry/Cell sorting to identify a potential role for pDC targeting in the control of the pathology of coronary artery disease.

Thu Nov 01 00:00:00 EDT 2012

Fates of CD4+ T cells in a tolerant environment depend on timing and place of antigen exposure.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Burrell BE,Bromberg JS

25-0691 was used in Flow cytometry/Cell sorting to elucidate the key factors that determine the fate of graft reactive T cells.

Thu Mar 01 00:00:00 EST 2012

Nitric oxide inhibits the accumulation of CD4+CD44hiTbet+CD69lo T cells in mycobacterial infection.

European journal of immunology

Pearl JE,Torrado E,Tighe M,Fountain JJ,Solache A,Strutt T,Swain S,Appelberg R,Cooper AM

25-0691 was used in Flow cytometry/Cell sorting to support the hypothesis that effector T cells in mycobacterial granulomata are not a uniform effector population but exist in distinct subsets with differential susceptibility to the regulatory effects of nitric oxide.

Sat Dec 01 00:00:00 EST 2012

Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice.

PloS one

Chow KP,Qiu JT,Lee JM,Hsu SL,Yang SC,Wu NN,Huang W,Wu TS

25-0691 was used in Flow cytometry/Cell sorting to investigate the unique IL-15-dependent cell expansion at the mature CD8SP stage of thymocyte development.

Mon Aug 20 00:00:00 EDT 2012

Debridement increases survival in a mouse model of subcutaneous anthrax.

PloS one

Weiner ZP,Boyer AE,Gallegos-Candela M,Cardani AN,Barr JR,Glomski IJ

25-0691 was used in Flow cytometry/Cell sorting to develop a mouse model of debridement to evaluate its effectiveness in the treatment of Bacillus anthracis soft tissue infections.

Mon Jul 23 00:00:00 EDT 2012

Interplay between CD8α+ dendritic cells and monocytes in response to Listeria monocytogenes infection attenuates T cell responses.

PloS one

Kapadia D,Sadikovic A,Vanloubbeeck Y,Brockstedt D,Fong L

25-0691 was used in Flow cytometry/Cell sorting to examine the contribution of different primary APCs during Listeria monocytogenes infection, showing interplay between dendritic cells and monocytes during infection.

Fri Apr 29 00:00:00 EDT 2011

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

25-0691 was used in Flow cytometry/Cell sorting to demonstrate that infection alters lung DCs for activation of antigen-specific lung T cells in response to airway antigen challenge.