Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), APC, eBioscience™

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

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CD71 (Transferrin Receptor) Antibody (17-0711-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.06 µg of Anti-Mouse CD71 (Transferrin Receptor) APC (right). Cells in the small scatter population were used for analysis.

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产品信息

17-0711-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看36篇已发表文章 36篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Fish, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

R17217 (RI7 217.1.4)

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1834355

产品详细信息

Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains.

Applications Reported: This R17217 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R17217 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors.

Cancer communications (London, England)

Hosaka K,Wang C,Zhang S,Lv X,Seki T,Zhang Y,Jing X,Wu J,Du Q,He X,Fan Y,Li X,Kondo M,Yoshihara M,Qian H,Shi L,Zhu P,Xu Y,Yang Y,Cheng T,Cao Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2023

Microbiota-assisted iron uptake promotes immune tolerance in the intestine.

Nature communications

Zhu L,Li G,Liang Z,Qi T,Deng K,Yu J,Peng Y,Zheng J,Song Y,Chang X

17-0711-82 was used in Flow cytometry/Cell sorting to reveal an association between nutrient uptake and immune tolerance in the intestine.

Mon May 15 00:00:00 EDT 2023

Nrf2 expands the intracellular pool of the chaperone AHSP in a cellular model of β-thalassemia.

Redox biology

Han G,Cao C,Yang X,Zhao GW,Hu XJ,Yu DL,Yang RF,Yang K,Zhang YY,Wang WT,Liu XZ,Xu P,Liu XH,Chen P,Xue Z,Liu DP,Lv X

17-0711-82 was used in flow cytometry experiments to demonstrate Nrf2-mediated feedback regulation of AHSP in response to excess α-globin, as occurs in β-thalassemia.

Fri Apr 01 00:00:00 EDT 2022

NSMF promotes the replication stress-induced DNA damage response for genome maintenance.

Nucleic acids research

Ju MK,Shin KJ,Lee JR,Khim KW,A Lee E,Ra JS,Kim BG,Jo HS,Yoon JH,Kim TM,Myung K,Choi JH,Kim H,Chae YC

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Engineered red blood cells carrying PCSK9 inhibitors persistently lower LDL and prevent obesity.

PloS one

Deshycka R,Sudaryo V,Huang NJ,Xie Y,Smeding LY,Choi MK,Ploegh HL,Lodish HF,Pishesha N

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Fri Dec 24 00:00:00 EST 2021

Metabolic requirements of NK cells during the acute response against retroviral infection.

Nature communications

Littwitz-Salomon E,Moreira D,Frost JN,Choi C,Liou KT,Ahern DK,O'Shaughnessy S,Wagner B,Biron CA,Drakesmith H,Dittmer U,Finlay DK

17-0711-82 was used in Flow cytometry/Cell sorting to show that following infection natural killer cells increase nutrient uptake, including amino acids and iron, and reprogram their metabolic machinery by increasing glycolysis and mitochondrial metabolism.

Fri Sep 10 00:00:00 EDT 2021

Evaluating the Role of the Steroid and Xenobiotic Receptor (SXR/PXR) in PCB-153 Metabolism and Protection against Associated Adverse Effects during Perinatal and Chronic Exposure in Mice.

Environmental health perspectives

Egusquiza RJ,Ambrosio ME,Wang SG,Kay KM,Zhang C,Lehmler HJ,Blumberg B

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

miR‑210 enhances mesenchymal stem cell‑modulated neural precursor cell migration.

Molecular medicine reports

Wang F,Zhu J,Zheng J,Duan W,Zhou Z

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cells.

eLife

Cendrowski J,Kaczmarek M,Mazur M,Kuzmicz-Kowalska K,Jastrzebski K,Brewinska-Olchowik M,Kominek A,Piwocka K,Miaczynska M

17-0711 was used in Flow cytometry/Cell sorting to investigate the role of the longer(L) and shorter(S) splicing variant of bone morphogenetic protein 2-inducible kinase(BMP2K) in endocytosis and cell differentiation.

Fri Aug 14 00:00:00 EDT 2020

Targeting Bim via a lncRNA Morrbid Regulates the Survival of Preleukemic and Leukemic Cells.

Cell reports

Cai Z,Aguilera F,Ramdas B,Daulatabad SV,Srivastava R,Kotzin JJ,Carroll M,Wertheim G,Williams A,Janga SC,Zhang C,Henao-Mejia J,Kapur R

17-0711 was used in Flow cytometry/Cell sorting to show that a lncRNA, MORRBID, a selective transcriptional repressor of BIM, is overexpressed in human acute myeloid leukemia (AML), which is associated with poor overall survival.

Tue Jun 23 00:00:00 EDT 2020

Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration.

Cell reports

Gadomski S,Singh SK,Singh S,Sarkar T,Klarmann KD,Berenschot M,Seaman S,Jakubison B,Gudmundsson KO,Lockett S,Keller JR

17-0711 was used in Flow cytometry/Cell sorting to investigate the effects of loss of ld genes on adult endothelial cell.

Tue Apr 28 00:00:00 EDT 2020

The Bone Marrow Protects and Optimizes Immunological Memory during Dietary Restriction.

Cell

Collins N,Han SJ,Enamorado M,Link VM,Huang B,Moseman EA,Kishton RJ,Shannon JP,Dixit D,Schwab SR,Hickman HD,Restifo NP,McGavern DB,Schwartzberg PL,Belkaid Y

17-0711-82 was used in Flow Cytometry to show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restriction (DR) but dramatically accumulated within the bone marrow (BM), where they adopted a state associated with energy conservation.

Thu Aug 22 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

JNK2 up-regulates hypoxia-inducible factors and contributes to hypoxia-induced erythropoiesis and pulmonary hypertension.

The Journal of biological chemistry

Sala MA,Chen C,Zhang Q,Do-Umehara HC,Wu W,Misharin AV,Waypa GB,Fang D,Budinger GRS,Liu S,Chandel NS,Schumacker PT,Sznajder JI,Liu J

17-0711-82 was used in Flow cytometry/Cell sorting to suggest that JNK2 is a positive regulator of HIFs and therefore may contribute to HIF-dependent pathologies.

Fri Jan 05 00:00:00 EST 2018

A cryptic cycle in haematopoietic niches promotes initiation of malaria transmission and evasion of chemotherapy.

Nature communications

Lee RS,Waters AP,Brewer JM

17-0711 was used in Flow cytometry/Cell sorting to enumerate Plasmodium infected erythropoietic precursor cells using an in vivo rodent model of Plasmodium berghei.

Fri Apr 27 00:00:00 EDT 2018

The Kinase mTORC1 Promotes the Generation and Suppressive Function of Follicular Regulatory T Cells.

Immunity

Xu L,Huang Q,Wang H,Hao Y,Bai Q,Hu J,Li Y,Wang P,Chen X,He R,Li B,Yang X,Zhao T,Zhang Y,Wang Y,Ou J,Liang H,Wu Y,Zhou X,Ye L

17-0711-82 was used in Flow Cytometry to study the effect of mTORC1 on Tfr cell differentiation by activating the TCF-1-Bcl-6 axis during immunization or infection.

Tue Sep 19 00:00:00 EDT 2017

TGF-β-Induced Quiescence Mediates Chemoresistance of Tumor-Propagating Cells in Squamous Cell Carcinoma.

Cell stem cell

Brown JA,Yonekubo Y,Hanson N,Sastre-Perona A,Basin A,Rytlewski JA,Dolgalev I,Meehan S,Tsirigos A,Beronja S,Schober M

17-0711 was used in Flow cytometry/Cell sorting to study the reversible entry of tumour-propagating cancer cells into a quiescent, chemoresistant state.

Thu Nov 02 00:00:00 EDT 2017

Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation.

Nucleic acids research

Zhang Y,Zhang J,An W,Wan Y,Ma S,Yin J,Li X,Gao J,Yuan W,Guo Y,Engel JD,Shi L,Cheng T,Zhu X

17071182 was used in flow cytometry to characterize a int-1-GATA knockout mouse

Wed Jan 25 00:00:00 EST 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.

Nature communications

Pasricha SR,Lim PJ,Duarte TL,Casu C,Oosterhuis D,Mleczko-Sanecka K,Suciu M,Da Silva AR,Al-Hourani K,Arezes J,McHugh K,Gooding S,Frost JN,Wray K,Santos A,Porto G,Repapi E,Gray N,Draper SJ,Ashley N,Soilleux E,Olinga P,Muckenthaler MU,Hughes JR,Rivella S,Milne TA,Armitage AE,Drakesmith H

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria.

G3 (Bethesda, Md.)

Huang HM,Bauer DC,Lelliott PM,Dixon MWA,Tilley L,McMorran BJ,Foote SJ,Burgio G

17-0711 was used in Flow cytometry/Cell sorting to describe the gene encoding erythrocytic ankyrin-1, and outline the resultant exhibition of allelic-dependent heterogeneous phenotypes during murine malarial infection.

Thu Sep 07 00:00:00 EDT 2017

The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.

Molecular and cellular biology

Ghanem LR,Kromer A,Silverman IM,Chatterji P,Traxler E,Penzo-Mendez A,Weiss MJ,Stanger BZ,Liebhaber SA

17-0711 was used in Flow cytometry/Cell sorting to provide direct evidence that Pcbp1 and Pcpb2 individually provide an essential function in the mammalian genome.

Fri Jan 15 00:00:00 EST 2016

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Molecular medicine reports

Hu Y,Zhang Y,Tian K,Xun C,Wang S,Lv D

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

The kinase Jnk2 promotes stress-induced mitophagy by targeting the small mitochondrial form of the tumor suppressor ARF for degradation.

Nature immunology

Zhang Q,Kuang H,Chen C,Yan J,Do-Umehara HC,Liu XY,Dada L,Ridge KM,Chandel NS,Liu J

17071182 was used in flow cytometry to study the role of Jnk2 in mitophagy.

Fri May 01 00:00:00 EDT 2015

Genome-wide association study follow-up identifies cyclin A2 as a regulator of the transition through cytokinesis during terminal erythropoiesis.

American journal of hematology

Ludwig LS,Cho H,Wakabayashi A,Eng JC,Ulirsch JC,Fleming MD,Lodish HF,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

17-0711 was used in Flow cytometry/Cell sorting to investigate role of the ubiquitously expressed Ant2 gene in animal development.

Tue Sep 01 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

17-0711 was used in Flow cytometry/Cell sorting to investigate role of the ubiquitously expressed Ant2 gene in animal development.

Tue Sep 01 00:00:00 EDT 2015

PARP-2 sustains erythropoiesis in mice by limiting replicative stress in erythroid progenitors.

Cell death and differentiation

Farrés J,Llacuna L,Martin-Caballero J,Martínez C,Lozano JJ,Ampurdanés C,López-Contreras AJ,Florensa L,Navarro J,Ottina E,Dantzer F,Schreiber V,Villunger A,Fernández-Capetillo O,Yélamos J

17-0711 was used in Flow cytometry/Cell sorting to highlight a role for PARP enzymes in the production of mature red blood cells.

Wed Jul 01 00:00:00 EDT 2015

A Systems Approach Identifies Essential FOXO3 Functions at Key Steps of Terminal Erythropoiesis.

PLoS genetics

Liang R,Campreciós G,Kou Y,McGrath K,Nowak R,Catherman S,Bigarella CL,Rimmelé P,Zhang X,Gnanapragasam MN,Bieker JJ,Papatsenko D,Ma'ayan A,Bresnick E,Fowler V,Palis J,Ghaffari S

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Inflammation and Fibrosis in Interferon Gamma Receptor-Deficient Mice.

PloS one

O'Flaherty BM,Matar CG,Wakeman BS,Garcia A,Wilke CA,Courtney CL,Moore BB,Speck SH

17-0711 was used in Flow cytometry/Cell sorting to investigate the role that the MHV68 gene plays in the development of pulmonary fibrosis.

Thu Jun 09 00:00:00 EDT 2016

Loss of IKKβ but Not NF-κB p65 Skews Differentiation towards Myeloid over Erythroid Commitment and Increases Myeloid Progenitor Self-Renewal and Functional Long-Term Hematopoietic Stem Cells.

PloS one

Zhang J,Li L,Baldwin AS,Friedman AD,Paz-Priel I

17-0711 was used in Flow cytometry/Cell sorting to study the effects of IKKβ and NF-κB p65 on hematopoietic lineage commitment and progenitor self-renewal and functional long-term hematopoietic stem cells.

Wed Apr 13 00:00:00 EDT 2016

Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication.

The Journal of experimental medicine

Nathanson DA,Armijo AL,Tom M,Li Z,Dimitrova E,Austin WR,Nomme J,Campbell DO,Ta L,Le TM,Lee JT,Darvish R,Gordin A,Wei L,Liao HI,Wilks M,Martin C,Sadeghi S,Murphy JM,Boulos N,Phelps ME,Faull KF,Herschman HR,Jung ME,Czernin J,Lavie A,Radu CG

17-0711 was used in Flow cytometry/Cell sorting to identify dCTP biosynthesis as a potential new therapeutic target for metabolic interventions in haematological malignancies.

Mon Mar 10 00:00:00 EDT 2014

Co-targeting of convergent nucleotide biosynthetic pathways for leukemia eradication.

The Journal of experimental medicine

17-0711 was used in Flow cytometry/Cell sorting to identify dCTP biosynthesis as a potential new therapeutic target for metabolic interventions in haematological malignancies.

Mon Mar 10 00:00:00 EDT 2014

mTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function.

Journal of immunology (Baltimore, Md. : 1950)

Donnelly RP,Loftus RM,Keating SE,Liou KT,Biron CA,Gardiner CM,Finlay DK

17-0711 was used in Flow cytometry/Cell sorting to investigate whether mTORC1-controlled metabolism underpins normal natural killer cell proinflammatory functions.

Sat Nov 01 00:00:00 EDT 2014

Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.

Blood

Hu T,Ghazaryan S,Sy C,Wiedmeyer C,Chang V,Wu L

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 17-0711-82) in Flow Cytometry

Thu May 10 00:00:00 EDT 2012

Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.

The Journal of experimental medicine

Austin WR,Armijo AL,Campbell DO,Singh AS,Hsieh T,Nathanson D,Herschman HR,Phelps ME,Witte ON,Czernin J,Radu CG

17-0711 was used in Flow cytometry/Cell sorting to investigate a functional link between nucleotide deficiency, replication stress, and two enzymes of the nucleoside salvage pathway: deoxycytidine kinase and thymidine kinase.