Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), PerCP-eFluor™ 710, eBioscience™

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

图: 1 / 27

CD71 (Transferrin Receptor) Antibody (46-0711-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.125 µg of Anti-Mouse CD71 (Transferrin Receptor) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

46-0711-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看22篇已发表文章 22篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Fish, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

R17217 (RI7 217.1.4)

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10854128

产品详细信息

Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains.

Applications Reported: This R17217 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R17217 (RI7 217.1.4) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors.

Cancer communications (London, England)

Hosaka K,Wang C,Zhang S,Lv X,Seki T,Zhang Y,Jing X,Wu J,Du Q,He X,Fan Y,Li X,Kondo M,Yoshihara M,Qian H,Shi L,Zhu P,Xu Y,Yang Y,Cheng T,Cao Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2023

NSMF promotes the replication stress-induced DNA damage response for genome maintenance.

Nucleic acids research

Ju MK,Shin KJ,Lee JR,Khim KW,A Lee E,Ra JS,Kim BG,Jo HS,Yoon JH,Kim TM,Myung K,Choi JH,Kim H,Chae YC

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Engineered red blood cells carrying PCSK9 inhibitors persistently lower LDL and prevent obesity.

PloS one

Deshycka R,Sudaryo V,Huang NJ,Xie Y,Smeding LY,Choi MK,Ploegh HL,Lodish HF,Pishesha N

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Fri Dec 24 00:00:00 EST 2021

Evaluating the Role of the Steroid and Xenobiotic Receptor (SXR/PXR) in PCB-153 Metabolism and Protection against Associated Adverse Effects during Perinatal and Chronic Exposure in Mice.

Environmental health perspectives

Egusquiza RJ,Ambrosio ME,Wang SG,Kay KM,Zhang C,Lehmler HJ,Blumberg B

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

miR‑210 enhances mesenchymal stem cell‑modulated neural precursor cell migration.

Molecular medicine reports

Wang F,Zhu J,Zheng J,Duan W,Zhou Z

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

TGF-β-Induced Quiescence Mediates Chemoresistance of Tumor-Propagating Cells in Squamous Cell Carcinoma.

Cell stem cell

Brown JA,Yonekubo Y,Hanson N,Sastre-Perona A,Basin A,Rytlewski JA,Dolgalev I,Meehan S,Tsirigos A,Beronja S,Schober M

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Thu Nov 02 00:00:00 EDT 2017

Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation.

Nucleic acids research

Zhang Y,Zhang J,An W,Wan Y,Ma S,Yin J,Li X,Gao J,Yuan W,Guo Y,Engel JD,Shi L,Cheng T,Zhu X

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Wed Jan 25 00:00:00 EST 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.

Nature communications

Pasricha SR,Lim PJ,Duarte TL,Casu C,Oosterhuis D,Mleczko-Sanecka K,Suciu M,Da Silva AR,Al-Hourani K,Arezes J,McHugh K,Gooding S,Frost JN,Wray K,Santos A,Porto G,Repapi E,Gray N,Draper SJ,Ashley N,Soilleux E,Olinga P,Muckenthaler MU,Hughes JR,Rivella S,Milne TA,Armitage AE,Drakesmith H

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria.

G3 (Bethesda, Md.)

Huang HM,Bauer DC,Lelliott PM,Dixon MWA,Tilley L,McMorran BJ,Foote SJ,Burgio G

46-0711 was used in Flow cytometry/Cell sorting to describe the gene encoding erythrocytic ankyrin-1, and outline the resultant exhibition of allelic-dependent heterogeneous phenotypes during murine malarial infection.

Thu Sep 07 00:00:00 EDT 2017

The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.

Molecular and cellular biology

Ghanem LR,Kromer A,Silverman IM,Chatterji P,Traxler E,Penzo-Mendez A,Weiss MJ,Stanger BZ,Liebhaber SA

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Fri Jan 15 00:00:00 EST 2016

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Molecular medicine reports

Hu Y,Zhang Y,Tian K,Xun C,Wang S,Lv D

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice.

Scientific reports

Huang HM,Bauer DC,Lelliott PM,Greth A,McMorran BJ,Foote SJ,Burgio G

46-0711 was used in Flow cytometry/Cell sorting to highlight the complex roles that ankyrin plays in mediating malarial resistance.

Wed Nov 16 00:00:00 EST 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Genome-wide association study follow-up identifies cyclin A2 as a regulator of the transition through cytokinesis during terminal erythropoiesis.

American journal of hematology

Ludwig LS,Cho H,Wakabayashi A,Eng JC,Ulirsch JC,Fleming MD,Lodish HF,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Erythrocytic Iron Deficiency Enhances Susceptibility to Plasmodium chabaudi Infection in Mice Carrying a Missense Mutation in Transferrin Receptor 1.

Infection and immunity

Lelliott PM,McMorran BJ,Foote SJ,Burgio G

46-0711 was used in Flow cytometry/Cell sorting to indicate that TFR1 deficiency alters erythrocyte physiology in a similar way to dietary iron deficiency anemia, promoting intraerythrocytic parasite survival in mice.

Sun Nov 01 00:00:00 EDT 2015

A Systems Approach Identifies Essential FOXO3 Functions at Key Steps of Terminal Erythropoiesis.

PLoS genetics

Liang R,Campreciós G,Kou Y,McGrath K,Nowak R,Catherman S,Bigarella CL,Rimmelé P,Zhang X,Gnanapragasam MN,Bieker JJ,Papatsenko D,Ma'ayan A,Bresnick E,Fowler V,Palis J,Ghaffari S

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 46-0711-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo.

Malaria journal

Lelliott PM,Lampkin S,McMorran BJ,Foote SJ,Burgio G

46-0711 was used in Flow cytometry/Cell sorting to describe a novel flow cytometric in vivo parasite invasion assay to try and develop a less resistent malaria treatment.