Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), PE-Cyanine7, eBioscience™

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

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CD115 (c-fms) Antibody (25-1152-82) in Flow

Staining of mouse bone marrow cells (cultured for 3 days with 50 ng/mL M-CSF (Product # 14-8331-80) with Anti-Mouse CD11b eFluor® 450 (Product # 48-0112-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Anti-Mouse CD115 (c-fms) PE-Cyanine7 (right). Cells in the large scatter population were used for analysis.

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产品信息

25-1152-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Mouse

已发表种属

Fish, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

AFS98

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573386

产品详细信息

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse BM derived macrophages. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.

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Dynamic intron retention modulates gene expression in the monocytic differentiation pathway.

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25-1152-82 was used in Flow cytometry/Cell sorting to highlight the unique role of intron retention in the regulation of the monocytic differentiation pathway.

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Transcriptional and Distributional Profiling of Microglia in Retinal Angiomatous Proliferation.

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25-1152-82 was used in Flow cytometry/Cell sorting to find that MG are the dominant myeloid cell population at RAP sites, significantly altering their transcriptional profile during RAP formation, however, without showing substantial upregulation of angiomodulatory factors.

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Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

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Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

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Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.

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Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.

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A new fate mapping system reveals context-dependent random or clonal expansion of microglia.

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Tay TL,Mai D,Dautzenberg J,Fernández-Klett F,Lin G,Datta M,Drougard A,Stempfl T,Ardura-Fabregat A,Staszewski O,Margineanu A,Sporbert A,Steinmetz LM,Pospisilik JA,Jung S,Priller J,Grün D,Ronneberger O,Prinz M

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Enforced Expression of Hoxa3 Inhibits Classical and Promotes Alternative Activation of Macrophages In Vitro and In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

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PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice.

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G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer.

Oncoimmunology

Hollmén M,Karaman S,Schwager S,Lisibach A,Christiansen AJ,Maksimow M,Varga Z,Jalkanen S,Detmar M

25-1152 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms by which tumour-associated macrophages promote breast cancer growth and metastasis, showing that G-CSF regulates macrophage phenotype and is associated with poor survival.

Tue Mar 01 00:00:00 EST 2016

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

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Fri Mar 01 00:00:00 EST 2013

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

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Mon Feb 25 00:00:00 EST 2013

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

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Fri Oct 24 00:00:00 EDT 2008

生物信息学

蛋白别名: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

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基因别名: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

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UniProt ID: (Mouse) P09581

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Entrez Gene ID: (Mouse) 12978

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功能