Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Alexa Fluor™ 488, eBioscience™

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

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CD115 (c-fms) Antibody (53-1152-82) in Flow

Staining of thioglycolate-induced peritoneal exudeate cells (PECs) with 0.25 µg of Rat IgG2a kappa Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (blue histogram) or 0.25 µg of Anti-Mouse CD115 (c-fms) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.

CD115 (c-fms) Antibody in Immunocytochemistry (ICC/IF)

CD115 (c-fms) Antibody in Immunohistochemistry (IHC)

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产品信息

53-1152-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Fish, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

AFS98

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2016696

产品详细信息

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse peritoneal elicited cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

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基因别名: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

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UniProt ID: (Mouse) P09581

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Entrez Gene ID: (Mouse) 12978

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功能