Invitrogen

CD45RA Monoclonal Antibody (HI100), eBioscience™

CD45RA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD45RA Antibody (14-0458-82) in IHC (P)

Immunohistochemical analysis of CD45RA was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Fo... View More

CD45RA Antibody in Flow Cytometry (Flow)

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产品信息

14-0458-82

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

查看25篇已发表文章 25篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

HI100

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_467272

产品详细信息

Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported: The HI100 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: The HI100 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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14-0458-82 was used in Flow cytometry/Cell sorting to identify a T cell-autonomous role for ZFYVE21 in promoting chronic vascular inflammation associated with allograft vasculopathy.

Tue Mar 21 00:00:00 EDT 2023

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

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Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

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ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis.

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Qian Y,Arellano G,Ifergan I,Lin J,Snowden C,Kim T,Thomas JJ,Law C,Guan T,Balabanov RD,Kaech SM,Miller SD,Choi J

14-0458-82 was used in Flow Cytometry to identify disease-relevant regulators of T cell fate by examining mutations that modify risk for multiple sclerosis.

Tue Aug 24 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

14-0458-82 was used in Flow Cytometry to explore the immunological changes in circulating Helios+ and Helios- regulator T-cells in type 1 diabetes.

Wed Sep 15 00:00:00 EDT 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

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Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

14-0458-82 was used in Flow Cytometry to explore the immunological changes in circulating Helios+ and Helios- regulator T-cells in type 1 diabetes.

Wed Sep 15 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

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Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

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14-0458-82 was used in Flow Cytometry to establish the recommended Phase II dose (RP2D). Secondary objectives included overall response rate (ORR), progression-free survival (PFS), and overall survival (OS).

Thu Jun 11 00:00:00 EDT 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

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MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

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Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

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Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia.

Nature communications

Bell CC,Fennell KA,Chan YC,Rambow F,Yeung MM,Vassiliadis D,Lara L,Yeh P,Martelotto LG,Rogiers A,Kremer BE,Barbash O,Mohammad HP,Johanson TM,Burr ML,Dhar A,Karpinich N,Tian L,Tyler DS,MacPherson L,Shi J,Pinnawala N,Yew Fong C,Papenfuss AT,Grimmond SM,Dawson SJ,Allan RS,Kruger RG,Vakoc CR,Goode DL,Naik SH,Gilan O,Lam EYN,Marine JC,Prinjha RK,Dawson MA

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Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection.

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The distribution and function of human memory T cell subsets in lung cancer.

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Published figure using CD45RA monoclonal antibody (Product # 14-0458-82) in Flow Cytometry

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TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

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Efficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9.

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Sun Feb 15 00:00:00 EST 2015

Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.

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生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

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