Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), Biotin, eBioscience™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

28个已发表图片

20 篇参考文献

查看其他109个Transferrin Receptor (CD71)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

图: 1 / 30

CD71 (Transferrin Receptor) Antibody (13-0719-82) in Flow

Staining of 3-day unstimulated (left) and 3-day PHA-stimulated (right) normal human peripheral blood cells with 0.06 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.06 µg of Anti-Human CD71 (Transferrin Receptor) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD71 (Transferrin Receptor) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD71 (Transferrin Receptor) Antibody in Immunocytochemistry (ICC/IF)

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产品信息

13-0719-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看18篇已发表文章 18篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

OKT9 (OKT-9)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466504

产品详细信息

Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Tested: This OKT9 (OKT-9) antibody has been tested by flow cytometric analysis of unstimulated and PHA-stimulated (3 days) human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Immunocytochemistry

Wed Aug 24 00:00:00 EDT 2022

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Immunocytochemistry

Wed Aug 24 00:00:00 EDT 2022

A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment.

NPJ precision oncology

Cornelison RC,Yuan JX,Tate KM,Petrosky A,Beeghly GF,Bloomfield M,Schwager SC,Berr AL,Stine CA,Cimini D,Bafakih FF,Mandell JW,Purow BW,Horton BJ,Munson JM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Fri Jul 29 00:00:00 EDT 2022

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Cell reports

Zaborowski MP,Lee K,Na YJ,Sammarco A,Zhang X,Iwanicki M,Cheah PS,Lin HY,Zinter M,Chou CY,Fulci G,Tannous BA,Lai CP,Birrer MJ,Weissleder R,Lee H,Breakefield XO

13-0719 was used in Flow cytometry/Cell sorting to develop a computational framework that integrates information about membrane proteins in tumours and normal tissues from databases.

Tue Apr 02 00:00:00 EDT 2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Cell reports

Zaborowski MP,Lee K,Na YJ,Sammarco A,Zhang X,Iwanicki M,Cheah PS,Lin HY,Zinter M,Chou CY,Fulci G,Tannous BA,Lai CP,Birrer MJ,Weissleder R,Lee H,Breakefield XO

13-0719 was used in Flow cytometry/Cell sorting to develop a computational framework that integrates information about membrane proteins in tumours and normal tissues from databases.

Tue Apr 02 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

Developmental cell

Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Mon Sep 10 00:00:00 EDT 2018

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

Cell stem cell

Giani FC,Fiorini C,Wakabayashi A,Ludwig LS,Salem RM,Jobaliya CD,Regan SN,Ulirsch JC,Liang G,Steinberg-Shemer O,Guo MH,Esko T,Tong W,Brugnara C,Hirschhorn JN,Weiss MJ,Zon LI,Chou ST,French DL,Musunuru K,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.

BMC genomics

Aparicio-Prat E,Arnan C,Sala I,Bosch N,Guigó R,Johnson R

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Fri Oct 23 00:00:00 EDT 2015

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Immunofluorescence

Thu May 02 00:00:00 EDT 2013

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 13-0719-82) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2011

生物信息学

蛋白别名: CD antigen CD71; CD71; p90; T9; TR; Transferrin receptor protein 1

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基因别名: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR

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UniProt ID: (Human) P02786

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Entrez Gene ID: (Human) 7037

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功能

virus receptor activity glycoprotein binding double-stranded RNA binding transferrin receptor activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding protein homodimerization activity poly(A) RNA binding chaperone binding metalloprotease

参与通路

regulation of cell growth response to hypoxia cellular iron ion homeostasis acute-phase response aging response to nutrient response to iron ion response to manganese ion osteoclast differentiation positive regulation of B cell proliferation receptor internalization response to retinoic acid transferrin transport cellular response to drug positive regulation of T cell proliferation regulation of cell proliferation positive regulation of bone resorption positive regulation of isotype switching response to copper ion viral entry into host cell iron ion import

CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), Biotin, eBioscience™

CD71 (Transferrin Receptor) Antibody (13-0719-82) in Flow

产品信息

13-0719-82

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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