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Invitrogen
FceR1 alpha Monoclonal Antibody (MAR-1), PE-eFluor™ 610, eBioscience™
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FceR1 alpha Antibody (61-5898-82) in Flow
Staining of mouse bone marrow-derived mast cells with 0.125 µg of Armenian Hamster IgG Isotype Control PE-eFluor® 610 (Product # 61-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse Fc epsilon Receptor I alpha (FceR1) PE-eFluor® 610 (purple histogram). Total viable cells as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
产品信息
61-5898-82
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
MAR-1
偶联物
激发/发射光谱
565/606 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574632
产品详细信息
Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported: This MAR-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MAR-1 antibody has been tested by flow cytometric analysis of mouse bone marrow-derived mast cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: fc epsilon r1; Fc-epsilon RI-alpha; FcERI; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; RP11-550P17.3
基因别名: Fce1a; fcepsilonri; Fcer1a; FcERI; Fcr-5
UniProt ID: (Mouse) P20489
Entrez Gene ID: (Mouse) 14125
activation of MAPK activity
positive regulation of type I hypersensitivity
serotonin secretion
signal transduction
cell surface receptor signaling pathway
activation of JUN kinase activity
leukotriene biosynthetic process
Fc-epsilon receptor signaling pathway
positive regulation of mast cell degranulation
positive regulation of interleukin-3 biosynthetic process
positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
