Invitrogen

CD4 Monoclonal Antibody (S3.5), Alexa Fluor™ 488

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5个已发表图片

7 篇参考文献

查看其他441个CD4抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 7

CD4 Antibody (MHCD0420) in Flow

Human peripheral blood lymphocytes were stained using Alexa Fluor®488 of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.

Product Image Gallery

CD4 Antibody — Figure 4 Antigen-specific CD8+ and CD4+ T cells response following stimulation by DRibbles or cell lysates. (A) The ubiquitinated proteins and pp65 proteins contained in the cell lysate and DRibbles were examined by western blot. Lysates and DRibbles extracted from cell lines treated with bortezomib contained more ubiquitinated proteins than those from non-treated cells (left). The pp65 protein was detected in the lysates and DRibbles extracted from the UbiLT3 pp65 cell line. The pp65 DRibbles collected from the bortezomib-treated UbiLT3 pp65 cell line contained more 148Kd pp65 protein compared with the other groups (right). Data are representative of 3 independent experiments. (B,C) PBMCs were stimulated by DRibbles and cell lysates at the following doses: 3ug/ml, 10ug/ml and 25ug/ml. The percentage of IFN-γ+CD8+(B) and IFN-γ+CD4+ cells (C) were calculated by flow cytometry. Percentages of IFN-γ+ T cells are shown as mean ± SEM. Data are representative of results from 3 independent experiments. (D) Dot plots at the antigen dose of 25ug/ml. {TM}

产品信息

MHCD0420

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

Assay-Dependent

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10372801

产品详细信息

Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent

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Product References

Exosomal Tat protein activates latent HIV-1 in primary, resting CD4+ T lymphocytes.

JCI insight

Tang X,Lu H,Dooner M,Chapman S,Quesenberry PJ,Ramratnam B

Published figure using CD4 monoclonal antibody (Product # MHCD0420) in Western Blot

Thu Apr 05 00:00:00 EDT 2018

Microfluidic differential immunocapture biochip for specific leukocyte counting.

Nature protocols

Hassan U,Watkins NN,Reddy B,Damhorst G,Bashir R

MHCD0420 was used in Immunohistochemistry to validate a novel biosensor based on a differential immunocapture technology to enumerate specific cells for diagnostic purposes.

Fri Apr 01 00:00:00 EDT 2016

Cross-presentation of viral antigens in dribbles leads to efficient activation of virus-specific human memory T cells.

Journal of translational medicine

Ye W,Xing Y,Paustian C,van de Ven R,Moudgil T,Hilton TL,Fox BA,Urba WJ,Zhao W,Hu HM

MHCD0420 was used in flow cytometry to study virus-specific human memory T-cell activation in response to Dribble-mediated cross-presentation of viral antigens.

Wed Apr 16 00:00:00 EDT 2014

Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit.

PloS one

Cavaretta JP,Sherer KR,Lee KY,Kim EH,Issema RS,Chung HJ

MHCD0420 was used in immunocytochemistry to identify the molecular mechanisms that regulate the enrichment of KCNQ channels at the neuronal axon

Tue Nov 10 00:00:00 EST 2015

Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit.

PloS one

Cavaretta JP,Sherer KR,Lee KY,Kim EH,Issema RS,Chung HJ

MHCD0420 was used in immunocytochemistry to identify the molecular mechanisms that regulate the enrichment of KCNQ channels at the neuronal axon

Tue Nov 10 00:00:00 EST 2015

Microfluidic CD4+ and CD8+ T lymphocyte counters for point-of-care HIV diagnostics using whole blood.

Science translational medicine

Watkins NN,Hassan U,Damhorst G,Ni H,Vaid A,Rodriguez W,Bashir R

MHCD0420 was used in flow cytometry to develop microfluidic biochips that count CD4(+) and CD8(+) lymphocytes in whole blood samples to use in third world countries.

Wed Dec 04 00:00:00 EST 2013

A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes.

Lab on a chip

Watkins NN,Sridhar S,Cheng X,Chen GD,Toner M,Rodriguez W,Bashir R

MHCD0420 was used in Flow cytometry/Cell sorting to develop a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations, with potential applications in quantitative HIV/AIDS diagnostics.