Invitrogen

CD4 Monoclonal Antibody (S3.5), Qdot™ 655

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CD4 Antibody (Q10007) in Flow

Qdot® 655 anti-human CD4 conjugate 655/20 band pass filter

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产品信息

Q10007

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

Qdot™ 655 Qdot™ 655 Qdot™ 655

激发/发射光谱

300/654 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

0.05M borate, pH 8.3, with 1M betaine

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11180600

产品详细信息

QdotTM Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, QdotTM Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10015.

Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10007: peak excitation 405 (488) nm/peak emission 655 nm; recommended filter 655/20 nm.

Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

T cell phenotypes in women with Chlamydia trachomatis infection and influence of treatment on phenotype distributions.

Microbes and infection

Ogendi BMO,Bakshi RK,Gupta K,Kapil R,Brown LT,Jordan SJ,Sabbaj S,Press CG,Lee JY,Geisler WM

Q10007 was used in Flow cytometry/Cell sorting to evaluate differences in T cell phenotypes between Chlamydia trachomatis(CT)-infected women and CT-seronegative controls and investigate changes in T cell phenotype distributions after CT treatment and their association with reinfection.

Thu Mar 01 00:00:00 EST 2018

PEGylated IL-10 (Pegilodecakin) Induces Systemic Immune Activation, CD8⁺ T Cell Invigoration and Polyclonal T Cell Expansion in Cancer Patients.

Cancer cell

Naing A,Infante JR,Papadopoulos KP,Chan IH,Shen C,Ratti NP,Rojo B,Autio KA,Wong DJ,Patel MR,Ott PA,Falchook GS,Pant S,Hung A,Pekarek KL,Wu V,Adamow M,McCauley S,Mumm JB,Wong P,Van Vlasselaer P,Leveque J,Tannir NM,Oft M

Q10007 was used in Flow cytometry/Cell sorting to report that pegilodecakin induces hallmarks of CD8+ T cell immunity in cancer patients, including elevation of interferon-γ and GranzymeB, expansion and activation of intratumoral CD8+ T cells, and proliferation and expansion of LAG-3+ PD-1+ CD8+ T cells.

Mon Nov 12 00:00:00 EST 2018

Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women.

American journal of reproductive immunology (New York, N.Y. : 1989)

Ogendi BMO,Bakshi RK,Sabbaj S,Brown L,Lee JY,Kapil R,Geisler WM

Q10007 was used in Flow cytometry/Cell sorting to characterise the distinct peripheral and mucosal T-cell phenotypes in chlamydia-infected women.

Fri Dec 01 00:00:00 EST 2017

Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody.

Immunological investigations

Kobie JJ,Treanor JJ,Ritchlin CT

Q10007 was used in flow cytometry to examine various DC after flu vaccination

Thu Apr 16 00:00:00 EDT 2015

Dual role of novel ingenol derivatives from Euphorbia tirucalli in HIV replication: inhibition of de novo infection and activation of viral LTR.

PloS one

Abreu CM,Price SL,Shirk EN,Cunha RD,Pianowski LF,Clements JE,Tanuri A,Gama L

Q10007 was used in Flow cytometry/Cell sorting to study the use of three ingenol synthetic derivatives to activate the HIV-LTR in latently infected cells whilst inhibiting replication.

Mon Jan 05 00:00:00 EST 2015

Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.

Clinical immunology (Orlando, Fla.)

McArthur MA,Sztein MB

Q10007 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which Staphylococcal enterotoxin B and other superantigens cause toxic shock syndrome.

Fri Feb 01 00:00:00 EST 2013

Leukocyte composition of human breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Ruffell B,Au A,Rugo HS,Esserman LJ,Hwang ES,Coussens LM

Q10007 was used in flow cytometry to examine tumor and nonadjacent normal breast tissue from women with breast cancer, who either had or had not received neoadjuvant chemotherapy before surgery