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Invitrogen
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
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Mouse IgG1 kappa Isotype Control (12-4714-42) in Flow
Normal human peripheral blood lymphocytes were stimulated with Cell Stimulation Cocktail plus protein transport inhibitors (Product # 00-4975) overnight. Cells were fixed and stained intracellularly with Anti-Human CD3 PerCP-Cyanine5.5 (Product # 45-0036-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-82) (left) or Anti-Human Amphiregulin PE (Product # 12-5370) (right) using the Intracellular Fix... View More
产品信息
12-4714-42
应用
建议稀释比
流式细胞分析 (Flow)
5 µL (0.5 µg)/test
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
P3.6.2.8.1
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1944423
产品详细信息
Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.
Applications Reported:PE Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested: Has been pre-titrated and tested by flow cytometric analysis. This can be used at test size: 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
