Invitrogen

PAI1 Polyclonal Antibody

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PAI1 Antibody (PA5-79980) in WB

Western blot analysis of PAI1 in Lane 1: rat small intestine tissue lysate, Lane 2: rat kidney tissue lysate, Lane 3: mouse kidney tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with PAI1 polyclonal antibody (Product # PA5-79980) at a dilution of 0.5... View More

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产品信息

PA5-79980

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

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免疫组化 (IHC)

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产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived rat PAI1 recombinant protein (Position: S24-D240).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2747095

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

EP₁ receptor antagonism mitigates early and late stage renal fibrosis.

Acta physiologica (Oxford, England)

Kresse JC,Mutsaers HAM,Jensen MS,Tingskov SJ,Madsen MG,Nejsum LN,Praetorius H,Nørregaard R

PA5-79980 was used in Western Blot, Immunohistochemistry to evaluate the role of the PGE2 -EP1 receptor in the pathogenesis of renal fibrosis and study selective EP1 receptor antagonist (SC-19220) as an anti-fibrotic for preventing the onset and late stage of renal fibrosis.

Tue Mar 01 00:00:00 EST 2022

EP₁ receptor antagonism mitigates early and late stage renal fibrosis.

Acta physiologica (Oxford, England)

Kresse JC,Mutsaers HAM,Jensen MS,Tingskov SJ,Madsen MG,Nejsum LN,Praetorius H,Nørregaard R

Tue Mar 01 00:00:00 EST 2022

EP₁ receptor antagonism mitigates early and late stage renal fibrosis.

Acta physiologica (Oxford, England)

Kresse JC,Mutsaers HAM,Jensen MS,Tingskov SJ,Madsen MG,Nejsum LN,Praetorius H,Nørregaard R

Tue Mar 01 00:00:00 EST 2022

Corticosteroid-binding-globulin (CBG)-deficient mice show high pY216-GSK3β and phosphorylated-Tau levels in the hippocampus.

PloS one

Gulfo J,Pérez de San Román J,Ledda A,Junyent F,Ramírez MJ,Gil-Bea FJ,Esteve M,Grasa M

PA5-79980 was used in Western Blotting to indicate that CBG deficiency triggers metabolic imbalance which could lead to damage and long-term neurological pathologies.

Wed Aug 04 00:00:00 EDT 2021

Corticosteroid-binding-globulin (CBG)-deficient mice show high pY216-GSK3β and phosphorylated-Tau levels in the hippocampus.

PloS one

Gulfo J,Pérez de San Román J,Ledda A,Junyent F,Ramírez MJ,Gil-Bea FJ,Esteve M,Grasa M

PA5-79980 was used in Western Blotting to indicate that CBG deficiency triggers metabolic imbalance which could lead to damage and long-term neurological pathologies.

Wed Aug 04 00:00:00 EDT 2021

生物信息学

蛋白别名: Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; PAI-1; plas; Plasminogen activator inhibitor 1; plasminogen activator inhibitor, type I; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, member 1; Serpin E1; SERPINE-1

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基因别名: PAI-1; PAI1; PAI1A; Pai1aa; Planh; Planh1; RATPAI1A; Serpine1

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UniProt ID: (Rat) P20961

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Entrez Gene ID: (Mouse) 18787, (Rat) 24617

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功能

protease binding serine-type endopeptidase inhibitor activity receptor binding protein binding peptidase inhibitor activity protease inhibitor

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