Invitrogen

PAI1 Monoclonal Antibody (242816)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

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PAI1 Antibody (MA5-23790) in WB

Western blot was performed using Anti-PAI1 Monoclonal Antibody (242816) (Product # MA5-23790) and a 43 and 45 kDa band corresponding to PAI1 was observed across cell lines tested . Whole cell extracts (30 µg lysate) of HAEC (Lane 1), HUVEC (Lane 2), A549 (Lane 3), Raji (Lane 4), U-2 OS (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed w... View More

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PAI1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PAI1 was observed as increased expression in HAEC and Huvec cell lines versus A-549, Raji and U-2 OS using Anti-PAI1 Monoclonal Antibody (242816) (Product # MA5-23790) in Western Blot. {RE}

产品信息

MA5-23790

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

中和实验 (Neu)

0.3 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

242816

抗原

Sf 21-derived recombinant human Serpin E1/PAI-1 Met1-Pro402

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2609712

产品详细信息

In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

靶标信息

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Endothelial plasminogen activator inhibitor; PAI; plas; Plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE-1

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基因别名: PAI; PAI-1; PAI1; PLANH1; SERPINE1

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UniProt ID: (Human) P05121

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Entrez Gene ID: (Human) 5054

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功能

protease binding serine-type endopeptidase inhibitor activity receptor binding protein binding calmodulin-related protease inhibitor

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