Invitrogen

PAI1 Monoclonal Antibody (MA-33H1F7)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

PAI1 Antibody in Immunocytochemistry (ICC/IF)

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PAI1 Antibody (MA1-40224) in ICC/IF

Immunofluorescence analysis of PAI1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PAI1 Monoclonal Antibody (MA-33H1F7) (Product # MA1-40224) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

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PAI1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PAI1 was observed as increased expression in HAEC and Huvec cell lines as compared to expression in A-549, Raji and U-2 OS using Anti-PAI1 Monoclonal Antibody (MA-33H1F7) (Product # MA1-40224) in Western Blot. {RE}

产品信息

MA1-40224

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:50

免疫细胞化学 (ICC/IF)

1:200

酶联免疫吸附实验 (ELISA)

1:50

功能检测 (Functional)

1:50

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

MA-33H1F7

抗原

Human PAI-1.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein G

保存液

PBS with 0.1% BSA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2186871

产品详细信息

MA1-40224 detects PAI-1 from human, rat, mouse and rabbit samples.

MA1-40224 has been successfully used in ELISA, functional studies and Western blot applications.

The MA1-40224 immunogen is human PAI-1.

Endotoxin <24 EU/mg.

The epitope (hF epitope) of MA1-40224 is predominantly composed of three residues (Lys154/Glu130/Arg131), positioned virtually linearly in the three-dimensional structure. The epitope of the antibody does not cover the complete alpha-helix F and turn connecting alpha-helix F and beta-strand s3A, but is restricted to the hinge region between alpha-helix F and the main part of the PAI-1 molecule. MA1-40224 is a ‘switching’ antibody, capable of inducing a non-inhibitory substrate form of PAI-1. It was shown to inhibit PAI-1 in a dose dependent manner.

靶标信息

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; PAI-1; plas; Plasminogen activator inhibitor 1; plasminogen activator inhibitor, type I; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); serine (or cysteine) peptidase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; serine (or cysteine) proteinase inhibitor, member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE-1

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基因别名: PAI; PAI-1; PAI1; PAI1A; Pai1aa; Planh; PLANH1; RATPAI1A; SERPINE1

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UniProt ID: (Human) P05121, (Rat) P20961

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Entrez Gene ID: (Human) 5054, (Mouse) 18787, (Rat) 24617

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功能

protease binding serine-type endopeptidase inhibitor activity receptor binding protein binding peptidase inhibitor activity calmodulin-related protease inhibitor

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