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- TaqMan 探针和 qPCR 引物
当赛默飞预设计的即用型TaqMan Assay无法满足您的实时荧光定量PCR研究需求时，TaqMan探针和qPCR引物成为您的不二之选。赛默飞提供一系列双标记TaqMan探针和用于qPCR引物的非标记寡核苷酸，研究人员可以根据各种实验需求灵活地选择合适的探针和引物。研究人员无论是使用定制探针通过生物信息学技术同时检测多个序列，还是检测尚未开发的预设计检测试剂的特异性靶标，赛默飞提供的出色探针都可用于设计个性化检测试剂盒。TaqMan MGB、TAMRA、QSY 和QSY2 定制探针均具备卓越的性能。这些定制qPCR探针和引物所使用的生产仪器设备和原材料与TaqMan检测试剂所用的相同，目前已被296,000多篇文献所引用，大大保证了探针和引物的质量与可靠性。
TaqMan probes
- 多重检测最多可检测6个靶标
- 可以轻松转换BHQ/Iowa Black 探针序列
- QSY探针用于多重检测，最多可检测4个靶标，可与ABY、JUN、FAM和VIC染料一起使用
- QSY2探针用于第5和第6个靶标的多重检测，使用花菁染料CY5和CY5.5
了解更多信息： 定制QSY和QSY2探针
TaqMan探针因其性能可靠，被广泛应用于各种研究应用中，尤其是定量PCR (qPCR) 实验。这些可定制的探针可以与靶标序列特异性结合，能够以高灵敏度对靶标核酸进行精准检测。TaqMan探针使用了双标记型式，探针的5’端是荧光基团，3’端是淬灭基团。在扩增过程中，探针被DNA聚合酶裂解，使得荧光基团和淬灭基团分离，从而释放荧光信号。荧光信号与样本中靶标核酸的含量成正比。TaqMan探针以其出色的质量、结果的可重复性高和化学成分的应用广泛而著称，对追求精准且可靠的qPCR实验结果的研究人员而言，TaqMan探针是值得信赖的选择。
什么是淬灭基团？
淬灭基团是一种可以抑制荧光基团发射出荧光信号的分子。这种抑制过程被称为淬灭，抑制作用可以通过多种机制发生，对于很多采用荧光标记的检测试剂盒和探针至关重要，包括用于基因分析和分子诊断的检测试剂盒和探针。
观看我们的Taq Talk系列视频，进一步了解qPCR
qPCR引物
- 提供干式和湿式两种规格
- 保证产量
- 选择交付规模
- 可用于所有实时荧光定量PCR或qPCR
了解更多信息： 序列检测引物
qPCR引物是分子生物学研究领域的重要工具，可以提高特定DNA序列扩增的精度和效率。序列检测引物是用于实时荧光定量PCR的定制引物，可与TaqMan探针或SYBR染料配合使用，可以灵活应用在多种实时荧光定量PCR研究应用中。序列检测引物可以根据各个实验设置的独特需求进行量身定制，有助于确保靶标扩增的准确性，从而提高检测的灵敏度和特异性，获得更加可靠、重复性更高的结果。此外，这些qPCR引物经过脱盐处理，有多种规格可供选择，既可以液体形式运输，也可以干燥后运输，为研究人员提供了极大的灵活性和便利性。定制的 qPCR 引物可在基因表达分析、基因分型和病原体检测等多种应用中展现出最佳性能。
让您的检测大获成功——qPCR数据的质量取决于所选的定制qPCR探针和引物的质量
- HPLC纯化探针——去除杂质，如未结合的染料或截短的探针，因为它们会增加背景信号并降低检测的灵敏度
- 可以选择以溶液形式运输——避免在重新悬浮过程中出现错误，从而改变试剂的浓度并严重影响下游实验
- 保证产量——确保为各种不同的样本量和规模提供适量的qPCR检测试剂盒
- 高效集成——TaqMan探针可以无缝集成到Applied Biosystems工作流程中，包括最新的预混液
*Estimated 4–10 days in North America. International times may vary.
TaqMan相关产品及服务
了解定制qPCR探针和qPCR引物相关实验和研究所需要的其他产品与服务
定制TaqMan探针在研究中的应用 |由Bioz提供支持
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Establishment and application of a qPCR method for differential detection of Brucella S2 vaccine strain. BMC veterinary research | 2025 Apr 03 | PubMed ID: 40176072 | Read Article "04):37–40 in Chinese. 28. Zhuo XJ. Immune measures of bovine and sheep brucellosis and its application prospect. Anim Ind Environ. 2024;08:70–2 in Chinese. 29. Gao ZQ, Xing J, Feng YF, Yue BF, He ZM. TaqMan MGB probe real-time PCR for rapid detection of Brucella. Chin J Zoonoses. 2011;27(11):995–1000 in Chinese. 30. Liu HW, Xu L, Fan Y, Bao GY, Chen JJ, Zhang TC. Study on the detection of Brucella ""on of sheep brucellosis. Xinjiang Xumuye. 2024;40(04):37–40 in Chinese. 28. Zhuo XJ. Immune measures of bovine and sheep brucellosis and its application prospect. Anim Ind Environ. 2024;08:70–2 in Chinese. 29. Gao ZQ, Xing J, Feng YF, Yue BF, He ZM. TaqMan MGB probe real-time PCR for rapid detection of Brucella. Chin J Zoonoses. 2011;27(11):995–1000 in Chinese. 30. Liu HW, Xu L, Fan Y, Bao GY, Chen JJ, Zhang TC. Study on the detection of Brucella nucleic acid in blood of patients with brucellosis" More... | |
The impact of ingestion of Bifidobacterium longum NCC3001 on perinatal anxiety and depressive symptoms: a randomized controlled trial Scientific Reports | 2025 Apr 02 | PubMed ID: 40175540 | Read Article "e QIAamp FAST DNA Stool Mini Kit (51604, Qiagen). DNA concentrations were measured using the PicoGreen fluorescence method (Thermo Fisher). The abundance of the BL NCC3001 strain was detected using a TaqMan MGB assay (Primer Express 3.0, Applied Biosystems) targeting a strain-specific chromosomic region that has been identified in previous studies 25 , 36 . The MasterMix LightCycler ® Multiplex DNA""en fluorescence method (Thermo Fisher). The abundance of the BL NCC3001 strain was detected using a TaqMan MGB assay (Primer Express 3.0, Applied Biosystems) targeting a strain-specific chromosomic region that has been identified in previous studies 25 , 36 . The MasterMix LightCycler ® Multiplex DNA Master (07339585001, Roche) was used with a final concentration of 0.9 µM for each primer and 0.25 µM of the probe. Each data point was run as technical triplicates and a standard curve was built in serial 10-fold dilution of BL NCC3001 genomic DNA. The assay was performed on a LC480 II cycler (Roche) using the following PCR conditions: 7 min at 95 °C for Taq activation, 10 s at 95 °C for denaturation and 30 s at 60 °C for annealing and extension x 40 cycles then the cooling 30 s at 40 °C. Another TaqMan MGB assay was used to normalize the BL NCC3001 abundance relative to the bacterial load (tota" More... | |
Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy. International journal of molecular sciences | 2025 Mar 27 | PubMed ID: 40141077 | Read Article "s separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqMan MGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 (Table S2)""by a 4 ◦C hold. One-third of the RT-PCR product was separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqMan MGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 (Table S2). RT-qPCR reactions were carried out with 2.0 µL o" More... | |
Methods and kits useful for diagnosis of human papillomavirus (HPV) Patent | 2025 Mar 25 | Read Article "Master Mix (2λ) and custom TaqMan™ Assay used in PCR was supplied by Thermofisher Scientific. The custom TaqMan™ Assay was composed as followings: Forward primer, Reverse primer and a FAM dye-labeled TaqMan MGB probe.
Plasma Cell Lysis
Each 2 mL of human pooled plasma was spiked with 100 fg 145 bp DNA+1.5 uL 2000 bp DNA (TATAA) stock. 160 uL of suitable lysis buffer and 60 uL of Proteinase K (28.5 ""s were of analytical grade.
TaqMan™ Fast Advanced Master Mix (2λ) and custom TaqMan™ Assay used in PCR was supplied by Thermofisher Scientific. The custom TaqMan™ Assay was composed as followings: Forward primer, Reverse primer and a FAM dye-labeled TaqMan MGB probe.
Plasma Cell Lysis
Each 2 mL of human pooled plasma was spiked with 100 fg 145 bp DNA+1.5 uL 2000 bp DNA (TATAA) stock. 160 uL of suitable lysis buffer and 60 uL of Proteinase K (28.5 mg/mL) was added into each 2 mL spiked plasma. The" More... | |
Novel mechanism for tubular injury in nephropathic cystinosis eLife | 2025 Mar 20 | PubMed ID: 40111391 | Read Article "eaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to Taqman MGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction "" used next day for quantitative Polymerase Chain Reaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to Taqman MGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction components, nuclease-free water, and primers to th" More... | |
Novel Mechanism for Tubular Injury in Nephropathic Cystinosis eLife | 2025 Mar 20 | Read Article "eaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to Taqman MGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction "" used next day for quantitative Polymerase Chain Reaction (qPCR). For qPCR, we designed two primers targeting two specific exons on CTNS gene – primer#1 targets between exon 2–3 and primer#2 targets between exon 9–10. These primers were connected to Taqman MGB probe (Thermo Fisher, Waltham, MA). We diluted the cDNA and used 1.25 ng for the qPCR reaction. Briefly, we added master mix (Applied Bioscience, Waltham, MA) that contains all the reaction components, nuclease- free water, and primers to t" More... | |
Sedimentary DNA is a promising indicator of the abundance of marine benthos: Insights from the burrowing decapod Upogebia major. PloS one | 2025 Mar 19 | PubMed ID: 40106512 | Read Article "ly related species U. yokoyai was explored and designated as the sequence for the speciesspecific primer-probe for U. major. The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqMan MGB Probe: UMPb1) sequences are listed in Table 3. The PCR amplification size achieved through PCR was 94 bp.
In order to validate the species-specificity of the designed primer-probe set, quant""imer-probe for U. major. The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqMan MGB Probe: UMPb1) sequences are listed in Table 3. The PCR amplification size achieved through PCR was 94 bp.
In order to validate the species-specificity of the designed primer-probe set, quantitative polymerase chain reactions (qPCR) were performed on genomic DNA extracted from the muscle tissues of U. major, its closely related species U. yokoyai, and the dominant species at each survey site, including Asari clam R. philippinarum, hermit crab Pagurus minutus and horn snail Batillaria attramentaria (refer to next paragraph for the composition of the qPCR reaction solution and PCR conditions). Only the DNA of U. major was amplified, substantiating its species specificity (S1 Fig).
Quantification of sedDNA concentration using qPCR The qPCR used in this study was performed using the StepOne Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). The total volume of the qPCR reaction mixture was 20 µL, which included 2 µL of DNA extraction solution, 10 µL of TaqMan
PLOS ONE | https://doi.org/10.1371/journal.pone.0318235 March 19, 2025 9 / 25
Fast Advanced Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), 1.8 uL each of 10 pmol/uL primers (final concentration 900 nM), 0.5 µL of 10 pmol/µL TaqMan MGB probe (250 nM), and 3.9 µL of nuclease-free water. qPCR was performed using a dilution se" More... | |
Sedimentary DNA is a promising indicator of the abundance of marine benthos: Insights from the burrowing decapod Upogebia major PLOS One | 2025 Mar 19 | Read Article " related species U. yokoyai was explored and designated as the sequence for the species-specific primer-probe for U. major . The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqMan MGB Probe: UMPb1) sequences are listed in Table 3 . The PCR amplification size achieved through PCR was 94 bp. In order to validate the species-specificity of the designed primer-probe set, qua""common to all groups and distinct from the closely related species U. yokoyai was explored and designated as the sequence for the species-specific primer-probe for U. major . The primer (forward primer: UMFW2208, Reverse primer: UMRV2208) and probe (TaqMan MGB Probe: UMPb1) sequences are listed in Table 3 . The PCR amplification size achieved through PCR was 94 bp. In order to validate the species-specificity of the designed primer-probe set, quantitative polymerase chain reactions (qPCR) were p" More... | |
Fabrication of 3D Biofunctional Magnetic Scaffolds by Combining Fused Deposition Modelling and Inkjet Printing of Superparamagnetic Iron Oxide Nanoparticles. Tissue engineering and regenerative medicine | 2025 Mar 18 | PubMed ID: 40100619 | Read Article "osystems, Thermo Fisher Scientific, Foster City, CA, USA). The expression levels of ALP and Runx2 were determined by qRT-PCR using the Taqman probes Hs01029144_m1 and Hs01047973_m1 (FAMTM dye-labeled TaqMan MGB probes, Applied Biosystems). For quantification of gene expression, the target gene values were normalized to the expression of the endogenous reference GAPDH (Glyceraldehyde 3-phosphate deh"" using a high-capacity RNA-to-cDNA kit (Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, USA). The expression levels of ALP and Runx2 were determined by qRT-PCR using the Taqman probes Hs01029144_m1 and Hs01047973_m1 (FAMTM dye-labeled TaqMan MGB probes, Applied Biosystems). For quantification of gene expression, the target gene values were normalized to the expression of the endogenous reference GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, Hs02786624_g1). The comparative thresh" More... | |
Sulforaphane-cysteine for wound healing in diabetic subjects Patent | 2025 Mar 18 | Read Article " Reverse Transcriptase Kit, Invitrogen) and amplified using the TaqMan Assay on Demand (Applied Biosystems) in a 25 μL reaction volume containing two unlabeled primers, a 6-carboxyfluorescien-labeled TaqMan MGB probe and the master mix. The amplified sequences were assessed using the ABI 7500 Prism Sequence Detection system machine. The results were expressed as mRNA levels normalized to 18S or GAP"" μg of RNA was reverse transcribed (Superscript II Reverse Transcriptase Kit, Invitrogen) and amplified using the TaqMan Assay on Demand (Applied Biosystems) in a 25 μL reaction volume containing two unlabeled primers, a 6-carboxyfluorescien-labeled TaqMan MGB probe and the master mix. The amplified sequences were assessed using the ABI 7500 Prism Sequence Detection system machine. The results were expressed as mRNA levels normalized to 18S or GAPDH in each sample.
Cell Transfection and Nrf2 Acti" More... | |
Regulation of serum reproductive hormones, gap junction proteins, and cytokine profiles in laying hens fed varying levels of expanded black soldier fly meal Poultry Science | 2025 Mar 16 | PubMed ID: 40120254 | Read Article "e gene (18S rRNA), was carried out in a 10 μL reaction volume run in duplicate. This mixture included 5 μL of TaqMan Gene Expression Master Mix, 0.5 μL of TaqMan Gene Expression Assay with a specific TaqMan MGB probe and primers (provided by Applied Biosystems), 0.5 μL of Eukaryotic 18S rRNA Endogenous Control (consisting of primers and a VIC/TAMRA-labeled TaqMan probe), 3 μL of water, and 1 μL of ""R, targeting the gene of interest and the reference gene (18S rRNA), was carried out in a 10 μL reaction volume run in duplicate. This mixture included 5 μL of TaqMan Gene Expression Master Mix, 0.5 μL of TaqMan Gene Expression Assay with a specific TaqMan MGB probe and primers (provided by Applied Biosystems), 0.5 μL of Eukaryotic 18S rRNA Endogenous Control (consisting of primers and a VIC/TAMRA-labeled TaqMan probe), 3 μL of water, and 1 μL of cDNA. A reaction without a DNA or cDNA template wa" More... | |
Mitochondrial mt12361A>G increased risk of metabolic dysfunction-associated steatotic liver disease among non-diabetes World Journal of Gastroenterology | 2025 Mar 14 | Read Article " protocols[ 39 - 41 ]. The Taqman ® allelic discrimination assay was used for genotyping the significant mtSNP in the validation dataset. The probe and primer sequences of mt12361A>G are as follows: TaqMan MGB probes: VIC-CACTACTATAACCACCCTAAC (wild type); FAM-ACTACTATAACCGCCCTAAC (variant); Forward primer: 5’-GGTGCAACTCCAAATAAAAGTAATAACCA-3’; Reverse primer: 5’-GTGGATGCGACAATGGATTTTACAT-3’. Genot""as performed according to the previously published protocols[ 39 - 41 ]. The Taqman ® allelic discrimination assay was used for genotyping the significant mtSNP in the validation dataset. The probe and primer sequences of mt12361A>G are as follows: TaqMan MGB probes: VIC-CACTACTATAACCACCCTAAC (wild type); FAM-ACTACTATAACCGCCCTAAC (variant); Forward primer: 5’-GGTGCAACTCCAAATAAAAGTAATAACCA-3’; Reverse primer: 5’-GTGGATGCGACAATGGATTTTACAT-3’. Genotyping was performed according to the manufacturer’" More... | |
Utilizing Functionalized Dual-Microbead Interfaces for Measurements of Amyloid-beta (1-42) with Femtomolar Sensitivity. Langmuir : the ACS journal of surfaces and colloids | 2025 Mar 11 | PubMed ID: 39993947 | Read Article "Abstract: The presence of amyloid-beta (1-42) in CSF and plasma has been implicated as a pathological hallmark of Alzheimer's disease. Here, we demonstrate a high-sensitivity method of detection and quantification for total Aβ42 utilizing two-antibody functionalized microparticles interfaces. Our method of biomarker detection utilizes two types of microbeads: (1) magnetic particles functionalized with monoclonal antibodies and (2) polystyrene particles dual functionalized with monoclonal antibodies and a unique DNA "tag"""Abstract: The presence of amyloid-beta (1-42) in CSF and plasma has been implicated as a pathological hallmark of Alzheimer's disease. Here, we demonstrate a high-sensitivity method of detection and quantification for total Aβ42 utilizing two-antibody functionalized microparticles interfaces. Our method of biomarker detection utilizes two types of microbeads: (1) magnetic particles functionalized with monoclonal antibodies and (2) polystyrene particles dual functionalized with monoclonal antibodies and a unique DNA "tag". The bead complex that forms in the presence of the target analyte is detected using qPCR to determine the analyte concentration. To demonstrate our method, we tested for the presence of amyloid-beta (1-42) in Tris buffer" More... | |
Using a novel gene site to develop a duplex real-time TaqMan MGB probe PCR method for the SNP detection and differentiation between the MS-H live vaccine strain and wild-type Mycoplasma synoviae strains Poultry Science | 2025 Mar 08 | Read Article "al 21 candidate detection targets were selected by conservativeness and specificity analyses, and were using in subsequent assays. Based on the targets screened above, we developed a duplex real-time TaqMan MGB probe PCR method utilizing a hydrolysis TaqMan oligonucleotide probe, specifically MGB probe. This probe loaded a reporter dye at the 5′-end and attached a NFQ quenching dyes at the 3′-end. ""re using in subsequent assays. Based on the targets screened above, we developed a duplex real-time TaqMan MGB probe PCR method utilizing a hydrolysis TaqMan oligonucleotide probe, specifically MGB probe. This probe loaded a reporter dye at the 5′-end and attached a NFQ quenching dyes at the 3′-end. The inclusion of the NFQ in the MGB probe significantly reduces background fluorescence ( Kutyavin et al., 2000 ). Through this, the highly stable interaction between the MGB probe and the target increases the melting temperature (Tm) of the probe and its specificity ( Sylvain et al., 2004 ; Kutyavin et al., 2000 ). This method is applicable in both single and multiplex formats for SNP detection across many species ( Kutyavin, 2010 ; Tomás et al., 2012 ), including Helicobacter pylori ( Zhao et al., 2022 ), Mucormycosis ( Bergallo et al., 2022 ), and avian influenza viruses ( Zhang et al., 2017 ). In this study, a pair of primers and two specific MGB probes were designed for the SNP mutation site of the ktrB gene, one probe was specific for attenuated vaccine strain MS-H and the other probe was for wild-type MS, which could simultaneously identify MS-H and wild-type MS in one reaction. Orthogonal experiments were conducted to determine the optimal primer and probe concentrations. The optimized reaction system not only can accurately distinguish vaccine strain MS-H from wild-type MS, but also can be used for clinical detection of MS infection. The specificity test revealed no non-specific reactions with other avian pathogens, demonstrating high specificity. In conjunction with the results from the interference test, it was further confirmed that the method could accurately identify the target strains even in the presence of a large number of clinically interfering strains. In the sensitivity test, the detection limits for MS-H and wild-type MS were 6.25 copies/μL and 14.4 copies/μL, respectively. Additionally, the detection limits for the simulated clinical samples contaminated with MS-H strain and wild-type MS were 5.75 CFU/mL and 1.0 × 10 2 CFU/mL, respectively. The sensitivity of the Taqman MGB method established using this ktrB gene exceeds that of existing methods. Dijkman ( Dijkm" More... | |
Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy International Journal of Molecular Sciences | 2025 Mar 08 | Read Article " separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqMan MGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 ( Table S2""y a 4 °C hold. One-third of the RT-PCR product was separated by agarose gel electrophoresis. Agarose gels contained 0.3 µg/mL of ethidium bromide (BioShop Canada, Burlington, ON, Canada), and PCR products were visualized under UV light. Primers and TaqMan MGB probes for RT-qPCR were designed with Primer Express Software version 2.0 (Thermo Fisher Scientific, Mississauga, ON, Canada) using default criteria for genes UMAG_00175 and uded1 ( Table S2 ). RT-qPCR reactions were carried out with 2.0 µL" More... | |
Using a novel gene site to develop a duplex real-time TaqMan MGB probe PCR method for the SNP detection and differentiation between the MS-H live vaccine strain and wild-type Mycoplasma synoviae strains. Poultry science | 2025 Mar 08 | PubMed ID: 40080948 | Read Article "Abstract: Mycoplasma synoviae (MS) is a globally prevalent avian pathogen responsible for airsacculitis and synovitis. The temperature-sensitive (ts)+ vaccine strain MS-H, a live attenuated variant, is the most effective and widely used vaccine for controlling infections in the poultry industry. Consequently, accurate detection is essential for a strategy known as differentiating infected from vaccinated animals (DIVA)""Abstract: Mycoplasma synoviae (MS) is a globally prevalent avian pathogen responsible for airsacculitis and synovitis. The temperature-sensitive (ts)+ vaccine strain MS-H, a live attenuated variant, is the most effective and widely used vaccine for controlling infections in the poultry industry. Consequently, accurate detection is essential for a strategy known as differentiating infected from vaccinated animals (DIVA). In this study, we developed a duplex real-time TaqMan minor groove binder (MGB) probe PCR (The DRTM-probe PCR) method to differentiate the MS-H live vaccine strain from wild-type strains by targeting a single nucleotide polymorphism (SNP) in the ktrB gene. This gene overcomes the restoration of the genotype of wild-type 86079/7NS in specific regions" More... | |
Real‐Time Environmental DNA | 2025 Mar 01 | Read Article "and Dawson 2010) in place of M. bella primers was used for each species as a control. Gel electrophoresis confirmed the correct amplicon size.
Subsequent specificity testing via qPCR incorporated the TaqMan MGB probe, using a QuantStudio 3 Real- Time PCR system (ThermoFisher Scientific Pty Ltd., Australia) (initial denaturation step of 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°"" and Schierwater 2003) and Aa_H16S_15141H (Bayha and Dawson 2010) in place of M. bella primers was used for each species as a control. Gel electrophoresis confirmed the correct amplicon size.
Subsequent specificity testing via qPCR incorporated the TaqMan MGB probe, using a QuantStudio 3 Real- Time PCR system (ThermoFisher Scientific Pty Ltd., Australia) (initial denaturation step of 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min). Each 10 μL reaction, run in triplica" More... | |
Sample manipulation and assay with rapid temperature change Patent | 2025 Feb 18 | Read Article ". Pat. No. 5,210,015, which is entirely incorporated herein by reference. Non-limiting examples of probes that can be useful in Q-PCR or RTQ-PCR reactions include TaqMan probes, TaqMan Tamara probes, TaqMan MGB probes, or Lion probes.
A variety of arrangements of quencher and fluorescent dye can be used when both are used. In the case of a molecular beacon, for example, a quencher is linked to one ""ed quantitative amplification are described in U.S. Pat. No. 5,210,015, which is entirely incorporated herein by reference. Non-limiting examples of probes that can be useful in Q-PCR or RTQ-PCR reactions include TaqMan probes, TaqMan Tamara probes, TaqMan MGB probes, or Lion probes.
A variety of arrangements of quencher and fluorescent dye can be used when both are used. In the case of a molecular beacon, for example, a quencher is linked to one end of an oligonucleotide capable of forming a hai" More... | |
Endurance swimming exacerbates mitochondrial myopathy in mice with high mtDNA deletions. Mitochondrion | 2025 Feb 14 | PubMed ID: 39956167 | Read Article "CT-3', reverse primer for mtDNA: 5'-GGTGGAATCGGGACCAGTAGGA-3') and probes (tetramethylrhodamine [TAMRA]-labeled probe for WT mtDNA: 5'-TCTGTAGCCCTTTTTGTCACATGATC-3' TAMRA, fluorescein amidite-labeled TaqMan MGB Probe for mtDNA: 5'-AACTGGTGTATGGGAGATTT-3' MGB) along with GeneAce Probe qPCR Mix II (NIPPON GENE, Tokyo, Japan) as the master mix. The PCR protocol is as follows: an initial incubation at "" primer for mtDNA: 5'- TTTCACTATGAAGCTAAGAGCGTTAACCT-3', reverse primer for mtDNA: 5'-GGTGGAATCGGGACCAGTAGGA-3') and probes (tetramethylrhodamine [TAMRA]-labeled probe for WT mtDNA: 5'-TCTGTAGCCCTTTTTGTCACATGATC-3' TAMRA, fluorescein amidite-labeled TaqMan MGB Probe for mtDNA: 5'-AACTGGTGTATGGGAGATTT-3' MGB) along with GeneAce Probe qPCR Mix II (NIPPON GENE, Tokyo, Japan) as the master mix. The PCR protocol is as follows: an initial incubation at 50 ºC for 120 s, followed by 90 ºC for 120 s, and " More... | |
A Honduran Prevalence Study on Soil-Transmitted Helminths Highlights Serological Antibodies to Tm-WAP49 as a Diagnostic Marker for Exposure to Human Trichuriasis. The American journal of tropical medicine and hygiene | 2025 Feb 11 | PubMed ID: 39933187 | Read Article "Abstract: Soil-transmitted helminth (STH) infections rank among the most prevalent communicable diseases of humans, yet detection of these parasites is mostly restricted to identifying active infection through fecal examinations. Currently, there are no commercial diagnostic tools to identify a prior whipworm or hookworm exposure, and the few serological assays for roundworm infection have not been well validated for crossreactivity or infections in humans. Such diagnostic restrictions limit the range of scientific and clinical questions that surround STH exposures and their implicated relationship to chronic diseases, such as autoimmunity, allergy, and cancer""Abstract: Soil-transmitted helminth (STH) infections rank among the most prevalent communicable diseases of humans, yet detection of these parasites is mostly restricted to identifying active infection through fecal examinations. Currently, there are no commercial diagnostic tools to identify a prior whipworm or hookworm exposure, and the few serological assays for roundworm infection have not been well validated for crossreactivity or infections in humans. Such diagnostic restrictions limit the range of scientific and clinical questions that surround STH exposures and their implicated relationship to chronic diseases, such as autoimmunity, allergy, and cancer. The goal of this investigation was to evaluate the diagnostic potential of 13 STH recombinant proteins. As there are no gold standard tests to verify positive STH antisera, we used sera from active STH-infected individuals in Honduras (measured by quantitative real-time polymerase chain reaction of helminth DNA in stool) and compared antibody recognition by both ELISA and western blot with nonendemic control sera from age-matched individuals in the United States split into screening and validation cohorts" More... | |
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Stylesheet for Classic Wide Template adjustments
仅供科研使用，不可用于诊断目的。