Organism TypePrimate - Non HumanThis is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region. The line was derived from the CV-1 cell line (ATCC CCL-70) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. GIBCO brand cell line is a clonal isolate (COS-7L) adapted to serum-free monolayer culture.
TissuesKidney
PhenotypeAdherent
Primaryno
ApplicationCell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transfection: Gene Silencing,Transient Transfection

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403.61

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12500096 DMEM/F-12, powder, 50 L Each -
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15140148 Penicillin-Streptomycin (10,000 U/mL), 20 mL Each
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18324020 Lipofectamine™ Transfection Reagent, 4 mL Each
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31985062 Opti-MEM™ I Reduced Serum Medium, 100 mL Each
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