Invitrogen

CD27 Monoclonal Antibody (O323), PE, eBioscience™

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CD27 Antibody (12-0279-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD27 PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0279-42

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看24篇已发表文章 24篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

O323

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10718394

产品详细信息

Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported: The O323 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.

Life science alliance

Verhoeven D,Grinwis L,Marsman C,Jansen MH,Van Leeuwen EM,Kuijpers TW

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Oxygen level is a critical regulator of human B cell differentiation and IgG class switch recombination.

Frontiers in immunology

Koers J,Marsman C,Steuten J,Tol S,Derksen NIL,Ten Brinke A,van Ham SM,Rispens T

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

CAR T cells targeting tumor-associated exons of glypican 2 regress neuroblastoma in mice.

Cell reports. Medicine

Li N,Torres MB,Spetz MR,Wang R,Peng L,Tian M,Dower CM,Nguyen R,Sun M,Tai CH,de Val N,Cachau R,Wu X,Hewitt SM,Kaplan RN,Khan J,St Croix B,Thiele CJ,Ho M

12-0279-42 was used in Flow cytometry/Cell sorting to investigate if RNA-seq data can be exploited to help identify tumor-associated exons that can be targeted by CAR T cell therapies.

Tue Jun 15 00:00:00 EDT 2021

Expression of CD27 and CD28 on γδ T cells from the peripheral blood of patients with allergic rhinitis.

Experimental and therapeutic medicine

Wang Q,Sun Q,Chen Q,Li H,Liu D

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

More than one antibody of individual B cells revealed by single-cell immune profiling.

Cell discovery

Shi Z,Zhang Q,Yan H,Yang Y,Wang P,Zhang Y,Deng Z,Yu M,Zhou W,Wang Q,Yang X,Mo X,Zhang C,Huang J,Dai H,Sun B,Zhao Y,Zhang L,Yang YG,Qiu X

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Perturbed CD8⁺ T cell immunity across universal influenza epitopes in the elderly.

Journal of leukocyte biology

Nguyen THO,Sant S,Bird NL,Grant EJ,Clemens EB,Koutsakos M,Valkenburg SA,Gras S,Lappas M,Jaworowski A,Crowe J,Loh L,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.

Immunity

Magri G,Comerma L,Pybus M,Sintes J,Lligé D,Segura-Garzón D,Bascones S,Yeste A,Grasset EK,Gutzeit C,Uzzan M,Ramanujam M,van Zelm MC,Albero-González R,Vazquez I,Iglesias M,Serrano S,Márquez L,Mercade E,Mehandru S,Cerutti A

12-0279 was used in Flow cytometry/Cell sorting to discover SIgM may emerge from pre-existing memory IgM+B cells and aid SIgA in anchoring highly diverse commensal communities to mucus.

Tue Jul 18 00:00:00 EDT 2017

Antibody Tumor Targeting Is Enhanced by CD27 Agonists through Myeloid Recruitment.

Cancer cell

Turaj AH,Hussain K,Cox KL,Rose-Zerilli MJJ,Testa J,Dahal LN,Chan HTC,James S,Field VL,Carter MJ,Kim HJ,West JJ,Thomas LJ,He LZ,Keler T,Johnson PWM,Al-Shamkhani A,Thirdborough SM,Beers SA,Cragg MS,Glennie MJ,Lim SH

12-0279 was used in Flow cytometry/Cell sorting to demonstrate the therapeutic advantage of using an immunomodulatory mAb to regulate lymphoid cells, which then recruit and activate myeloid cells for enhanced killing of mAb-opsonized tumors.

Mon Dec 11 00:00:00 EST 2017

Maintenance of the EBV-specific CD8⁺ TCRαβ repertoire in immunosuppressed lung transplant recipients.

Immunology and cell biology

Nguyen TH,Bird NL,Grant EJ,Miles JJ,Thomas PG,Kotsimbos TC,Mifsud NA,Kedzierska K

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.

Oncoimmunology

Su S,Zou Z,Chen F,Ding N,Du J,Shao J,Li L,Fu Y,Hu B,Yang Y,Sha H,Meng F,Wei J,Huang X,Liu B

12-0279 was used in Flow cytometry/Cell sorting to study PD-1/PD-L1-mediated immune tolerance of Epstein-Barr virus-associated gastric cancer and provide a new strategy to break the tolerance for the T cell-based adoptive therapy.

Tue Jan 12 00:00:00 EST 2021

Inclusion of Strep-tag II in design of antigen receptors for T-cell immunotherapy.

Nature biotechnology

Liu L,Sommermeyer D,Cabanov A,Kosasih P,Hill T,Riddell SR

12-0279 was used in Flow cytometry/Cell sorting to introduce Strep-tag II sequences into synthetic chimeric antigen receptors or natural TCRs to facilitate in vivo tracking and retrieval of transferred cells used in immunotherapy.

Fri Apr 01 00:00:00 EDT 2016

Detection of impaired IgG antibody formation facilitates the decision on early immunoglobulin replacement in hypogammaglobulinemic patients.

Frontiers in immunology

Wolf HM,Thon V,Litzman J,Eibl MM

12-0279 was used in Flow cytometry/Cell sorting to evaluate the contribution of defective IgG antibody production in hypogammaglobulinemic patients.

Fri Feb 20 00:00:00 EST 2015

Surface Plasmon Resonance Analysis Shows an IgG-Isotype-Specific Defect in ABO Blood Group Antibody Formation in Patients with Common Variable Immunodeficiency.

Frontiers in immunology

Fischer MB,Wolfram W,Binder CJ,Böhmig GA,Wahrmann M,Eibl MM,Wolf HM

12-0279 was used in Flow cytometry/Cell sorting to prove that conventional isoagglutinin and anti-A/B IgM antibody assessments are not suited for the diagnosis of impaired antibody production.

Fri May 22 00:00:00 EDT 2015

Effect of Leflunomide on the Abnormal Expression of Lipid Rafts and F-Actin in B Lymphocytes from Patients with Systemic Lupus Erythematosus.

Journal of immunology research

Dong GF,Zhang X,He DN,Li L,Zhang GF

12-0279 was used in Immunofluorescence to demonstrate that the modulation of B cell expression by leflunomide could be a potential therapeutic target for systemic lupus erythematosus.

Thu Mar 03 00:00:00 EST 2016

Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Weiskopf D,Angelo MA,de Azeredo EL,Sidney J,Greenbaum JA,Fernando AN,Broadwater A,Kolla RV,De Silva AD,de Silva AM,Mattia KA,Doranz BJ,Grey HM,Shresta S,Peters B,Sette A

12-0279 was used in Flow cytometry/Cell sorting to analyse dengue virus-specific responses, supporting an HLA-linked protective role for CD8+ T cells.

Tue May 28 00:00:00 EDT 2013

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Long-term immune reconstitution of naive and memory t cell pools after haploidentical hematopoietic stem cell transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Azevedo RI,Soares MV,Albuquerque AS,Tendeiro R,Soares RS,Martins M,Ligeiro D,Victorino RM,Lacerda JF,Sousa AE

12-0279 was used in Flow cytometry/Cell sorting to show that long-term immune reconstitution was successfully achieved after haploidentical hematopoietic stem cell transplantation, a process that appears to have largely relied on de novo T cell production.

Wed May 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Reciprocal polarization of T and B cells at the immunological synapse.

Journal of immunology (Baltimore, Md. : 1950)

Duchez S,Rodrigues M,Bertrand F,Valitutti S

12-0279 was used in Flow cytometry/Cell sorting to study how B cell activation status can fine-tune T cell polarisation responses at the immunological synapse.

Tue Nov 01 00:00:00 EDT 2011

Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.

Biotechnology and bioengineering

Kuzin I,Sun H,Moshkani S,Feng C,Mantalaris A,Wu JH,Bottaro A

Published figure using CD27 monoclonal antibody (Product # 12-0279-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2011

Alterations on peripheral blood B-cell subpopulations in very early arthritis patients.

Rheumatology (Oxford, England)

Moura RA,Weinmann P,Pereira PA,Caetano-Lopes J,Canhão H,Sousa E,Mourão AF,Rodrigues AM,Queiroz MV,Souto-Carneiro MM,Graça L,Fonseca JE

12-0279 was used in Flow cytometry/Cell sorting to characterise circulating B-cell subpopulations in samples from arthritis patients within 6 weeks of the onset of disease.

Tue Jun 01 00:00:00 EDT 2010

Agricultural pesticide exposure and the molecular connection to lymphomagenesis.

The Journal of experimental medicine

Agopian J,Navarro JM,Gac AC,Lecluse Y,Briand M,Grenot P,Gauduchon P,Ruminy P,Lebailly P,Nadel B,Roulland S

12-0279 was used in Flow cytometry/Cell sorting to investigate the molecular connection between agricultural pesticide use, t(14;18) in blood and lymphomagenesis.

Mon Jul 06 00:00:00 EDT 2009

生物信息学

蛋白别名: CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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基因别名: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842

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Entrez Gene ID: (Human) 939

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功能

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process

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