Invitrogen

CD3 Monoclonal Antibody (7D6), FITC

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CD3 Antibody (MHCD0301) in Flow

Human peripheral blood lymphocytes stained using FITC of anti-human CD3 monoclonal antibody (clone S4.1). The negative control profiles represent unstained cells.

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产品信息

MHCD0301

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看8篇已发表文章 8篇已发表文章

其他PubMed (Misc)

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

7D6

抗原

Human CD3 epsilon

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10376003

产品详细信息

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targeting.

Nature communications

Bear AS,Blanchard T,Cesare J,Ford MJ,Richman LP,Xu C,Baroja ML,McCuaig S,Costeas C,Gabunia K,Scholler J,Posey AD,O'Hara MH,Smole A,Powell DJ,Garcia BA,Vonderheide RH,Linette GP,Carreno BM

MHCD0301 was used in Flow Cytometry to characterize HLA class I-restricted mutated KRAS epitopes in cancer.

Fri Jul 16 00:00:00 EDT 2021

Polyreactive Broadly Neutralizing B cells Are Selected to Provide Defense against Pandemic Threat Influenza Viruses.

Immunity

Guthmiller JJ,Lan LY,Fernández-Quintero ML,Han J,Utset HA,Bitar DJ,Hamel NJ,Stovicek O,Li L,Tepora M,Henry C,Neu KE,Dugan HL,Borowska MT,Chen YQ,Liu STH,Stamper CT,Zheng NY,Huang M,Palm AE,García-Sastre A,Nachbagauer R,Palese P,Coughlan L,Krammer F,Ward AB,Liedl KR,Wilson PC

Published figure using CD3 monoclonal antibody (Product # MHCD0301) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Polyreactive Broadly Neutralizing B cells Are Selected to Provide Defense against Pandemic Threat Influenza Viruses.

Immunity

Published figure using CD3 monoclonal antibody (Product # MHCD0301) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Ligand-receptor interaction atlas within and between tumor cells and T cells in lung adenocarcinoma.

International journal of biological sciences

Chen Z,Yang X,Bi G,Liang J,Hu Z,Zhao M,Li M,Lu T,Zheng Y,Sui Q,Yang Y,Zhan C,Jiang W,Wang Q,Tan L

MHCD0301 was used in Flow cytometry/Cell sorting to investigate tumor cell-T cell interactions in promoting tumor progression.

Mon Nov 15 00:00:00 EST 2021

Ligand-receptor interaction atlas within and between tumor cells and T cells in lung adenocarcinoma.

International journal of biological sciences

Chen Z,Yang X,Bi G,Liang J,Hu Z,Zhao M,Li M,Lu T,Zheng Y,Sui Q,Yang Y,Zhan C,Jiang W,Wang Q,Tan L

MHCD0301 was used in Flow cytometry/Cell sorting to investigate tumor cell-T cell interactions in promoting tumor progression.

Mon Nov 15 00:00:00 EST 2021

Monoclonal Antibody Responses after Recombinant Hemagglutinin Vaccine versus Subunit Inactivated Influenza Virus Vaccine: a Comparative Study.

Journal of virology

Henry C,Palm AE,Utset HA,Huang M,Ho IY,Zheng NY,Fitzgerald T,Neu KE,Chen YQ,Krammer F,Treanor JJ,Sant AJ,Topham DJ,Wilson PC

MHCD0301 was used in Flow cytometry/Cell sorting to compare the fine specificity of the antibodies induced by a recombinant HA vaccine produced in insect cells (Flublok) and Flucelvax, prepared from virions produced in mammalian cells.

Fri Nov 01 00:00:00 EDT 2019

Identification of a human monoclonal antibody to replace equine diphtheria antitoxin for treatment of diphtheria intoxication.

Infection and immunity

Sevigny LM,Booth BJ,Rowley KJ,Leav BA,Cheslock PS,Garrity KA,Sloan SE,Thomas W,Babcock GJ,Wang Y

MHCD0301 was used in Flow cytometry/Cell sorting to report on how the discovery of a specific and potent human neutralizing antibody against diphtheria toxin holds promise as a potential therapeutic.

Fri Nov 01 00:00:00 EDT 2013

Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen.

Nature protocols

Smith K,Garman L,Wrammert J,Zheng NY,Capra JD,Ahmed R,Wilson PC

MHCD0301 was used in Flow cytometry/Cell sorting to develop a protocol for the production of antigen-specific human monoclonal antibodies.

Wed Apr 08 00:00:00 EDT 2009

Inhibition of the Bcl-xL deamidation pathway in myeloproliferative disorders.

The New England journal of medicine

Zhao R,Follows GA,Beer PA,Scott LM,Huntly BJ,Green AR,Alexander DR

MHCD0301 was used in Flow cytometry/Cell sorting to study how BCR-ABL and mutant JAK2 inhibit the Bcl-x(L) deamidation pathway and the apoptotic response to DNA damage in primary cells from patients with CML or polycythemia vera.

Thu Dec 25 00:00:00 EST 2008

Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.

The Journal of infectious diseases

Asin SN,Fanger MW,Wildt-Perinic D,Ware PL,Wira CR,Howell AL

MHCD0301 was used in Flow Cytometry to characterise the life-cycle events of human immunodeficiency virus type 1 in primary cultures of human uterine epithelial cells and stromal fibroblasts.

Thu Jul 15 00:00:00 EDT 2004

Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.

Journal of immunological methods

Canonico B,Zamai L,Burattini S,Granger V,Mannello F,Gobbi P,Felici C,Falcieri E,Reilly JT,Barnett D,Papa S

MHCD0301 was used in flow cytometry to assess the effects of a TransFix-based stabilization technique on leukocyte scatter characteristics, immunophenotyping, membrane permeability, absolute cell counting and morphology.

Wed Dec 01 00:00:00 EST 2004

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Cytometry. Part B, Clinical cytometry

Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G

MHCD0301 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.

Tue Jul 01 00:00:00 EDT 2003

Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols.

Journal of immunological methods

Kappelmayer J,Gratama JW,Karászi E,Menéndez P,Ciudad J,Rivas R,Orfao A

MHCD0301 was used in flow cytometry to optimize B- and T-lymphoid lineage detection in acute leukemias by intracellular staining for myeloperoxidase, CD79a, and CD3.

Mon Aug 28 00:00:00 EDT 2000

T cell priming enhances IL-4 gene expression by increasing nuclear factor of activated T cells.

Journal of immunology (Baltimore, Md. : 1950)

Cron RQ,Bort SJ,Wang Y,Brunvand MW,Lewis DB

MHCD0301 was used in flow cytometry to study selective expression of cytokines by T cells.

Fri Jan 15 00:00:00 EST 1999

Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Paz-Miguel JE,Flores R,Sánchez-Velasco P,Ocejo-Vinyals G,Escribano de Diego J,López de Rego J,Leyva-Cobián F

MHCD0301 was used in flow cytometry to elucidate role of SOD-1 overexpression in thymocyte biology.

Mon Nov 15 00:00:00 EST 1999

生物信息学

蛋白别名: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

查看更多收起

基因别名: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ