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GABBR1 Antibody (MA5-45536) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GABA-B Receptor 1 monoclonal antibody (Product # MA5-45536) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red... View More
产品信息
MA5-45536
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N93A/49
抗原
Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931990
产品详细信息
1 µg/mL of MA5-45536 was sufficient for detection of GABA(B)R1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 115kDa. No cross-reactivity against GABA(B)R2.
This antibody was formerly sold as clone S93A-49.
靶标信息
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bM573K1.1.1 (gamma-aminobutyric acid (GABA) B receptor, 1a); DAAP-188P13.3; dJ271M21.1.1; dJ271M21.1.2; FLJ92613; GABA(B) receptor; GABA-B receptor 1; GABA-B receptor, R1 subunit; GABA-B-R1; Gaba-b-r1a; Gaba-b1; GABA-BR1; Gaba-br1a receptor; GABA-BR1b receptor; GABAB; GABABR1; Gababr1 receptor; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) B receptor, 1; gamma-aminobutyric acid (GABA-B) receptor, 1; Gamma-aminobutyric acid type B receptor subunit 1; gb1; GBR1; HGB1a; OTTHUMP00000109099; OTTHUMP00000214725; seven transmembrane helix receptor
基因别名: bM573K1.1; GABAB1; GABABR1; GABBR1; GABBR1-3; GB1; GPRC3A
UniProt ID: (Human) Q9UBS5, (Mouse) Q9WV18, (Rat) Q9Z0U4
Entrez Gene ID: (Human) 2550, (Mouse) 54393, (Rat) 81657
osteoblast differentiation
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
negative regulation of adenylate cyclase activity
gamma-aminobutyric acid signaling pathway
negative regulation of cell proliferation
positive regulation of glutamate secretion
negative regulation of gamma-aminobutyric acid secretion
negative regulation of epinephrine secretion
negative regulation of dopamine secretion
response to nicotine
response to ethanol
negative regulation of synaptic transmission
positive regulation of growth hormone secretion
signal transduction
G-protein coupled receptor signaling pathway
regulation of glutamate secretion
regulation of cAMP biosynthetic process
