Invitrogen

GAL4 Monoclonal Antibody

GAL4 Antibody in Immunocytochemistry (ICC/IF)

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GAL4 Antibody (33-8600) in ICC/IF

Immunofluorescent analysis of Gal4 (green) in Gal4 DNA Binding Domain expressing 293T cells (Product # K1091A). The cells were fixed with formalin for 20 minutes, permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Gal4 mouse monoclonal antibody (Product # 33-8600), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a di... View More

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产品信息

33-8600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫细胞化学 (ICC/IF)

1:50 - 1:100

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酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Yeast

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

抗原

A recombinant protein corresponding to amino acids 1-147 mapping within the amino terminal, DNA binding domain of GAL4.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533143

产品详细信息

33-8600 detects the Gal4 DNA Binding Domain by western blot, immunoprecipitation and immunofluorescence corresponding to amino acids 1-147 of the GAL4 DNA binding region.

靶标信息

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a "zinc finger" sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

仅用于科研。不用于诊断过程。未经明确授权不得转售。