Invitrogen

IL-17A Monoclonal Antibody (eBio64DEC17), PE-Cyanine7, eBioscience™

IL-17A Antibody in Flow Cytometry (Flow)

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IL-17A Antibody (25-7179-42) in Flow

CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23 Recombinant Protein (Product # 14-8239-63) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (left) or Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 6 hours. Cells were intracellularly stained with Anti-Human CD4 APC (Product # 17-0049-42) and Anti-Human IL-17A PE-Cyanine7 using the Fixation & Permeabilization Buffers (Product # 88-8823-88). V... View More

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产品信息

25-7179-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看27篇已发表文章 27篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Hamster, Human, Mouse, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio64DEC17

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11063994

产品详细信息

Description: The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13, an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies.

Applications Reported: This eBio64DEC17 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio64DEC17 antibody has been pre-titrated and tested by intracellular stainign and flow cytometric analysis. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution () for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4⁺ T cells.

Cell reports

Chatterjee D,Zhang Y,Ngassaki-Yoka CD,Dutilleul A,Khalfi S,Hernalsteens O,Wiche Salinas TR,Dias J,Chen H,Smail Y,Goulet JP,Bell B,Routy JP,Van Lint C,Ancuta P

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Tue Jun 27 00:00:00 EDT 2023

Cathepsin S inhibition in dendritic cells prevents Th17 cell differentiation in perivascular adipose tissues following vascular injury in diabetic rats.

Journal of biochemical and molecular toxicology

Peng H,Lv Y,Li C,Cheng Z,He S,Wang C,Liu J

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.

Molecular therapy. Nucleic acids

Chang GP,Yang XL,Liu W,Lin S,Yang SL,Zhao MY

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Small molecule allosteric inhibitors of RORγt block Th17-dependent inflammation and associated gene expression in vivo.

PloS one

Saenz SA,Local A,Carr T,Shakya A,Koul S,Hu H,Chourb L,Stedman J,Malley J,D'Agostino LA,Shanmugasundaram V,Malona J,Schwartz CE,Beebe L,Clements M,Rajaraman G,Cho J,Jiang L,Dubrovskiy A,Kreilein M,Shimanovich R,Hamann LG,Escoubet L,Ellis JM

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Wed Dec 29 00:00:00 EST 2021

YY1 regulation by miR-124-3p promotes Th17 cell pathogenicity through interaction with T-bet in rheumatoid arthritis.

JCI insight

Lin J,Tang J,Lin J,He Y,Yu Z,Jiang R,Yang B,Ou Q

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Mon Nov 22 00:00:00 EST 2021

Accumulation of TNFR2-expressing regulatory T cells in malignant pleural effusion of lung cancer patients is associated with poor prognosis.

Annals of translational medicine

Ye LL,Peng WB,Niu YR,Xiang X,Wei XS,Wang ZH,Wang X,Zhang SY,Chen X,Zhou Q

25-7179 was used in Flow cytometry/Cell sorting to investigate associations between TNFR2+Tregs frequencies present in malignant pleural effusion and the clinical and laboratorial characteristics of patients with lung cancer.

Tue Dec 01 00:00:00 EST 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA.

Cell reports

Agarwal S,Kraus Z,Dement-Brown J,Alabi O,Starost K,Tolnay M

25-7179 was used in Flow cytometry/Cell sorting to study the functional regulation of T cells by human Fc receptor-like 3.

Tue Feb 04 00:00:00 EST 2020

CXCL4 is a novel inducer of human Th17 cells and correlates with IL-17 and IL-22 in psoriatic arthritis.

European journal of immunology

Affandi AJ,Silva-Cardoso SC,Garcia S,Leijten EFA,van Kempen TS,Marut W,van Roon JAG,Radstake TRDJ

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Activated T follicular helper-like cells are released into blood after oral vaccination and correlate with vaccine specific mucosal B-cell memory.

Scientific reports

Cárdeno A,Magnusson MK,Quiding-Järbrink M,Lundgren A

25-7179 was used in Flow cytometry/Cell sorting to investigate the frequencies, phenotype and function of circulating Tfh-like CD4+CXCR5+T cells in adults receiving an oral inactivated enterotoxigenic Escherichia coli vaccine.

Fri Feb 09 00:00:00 EST 2018

Enhancing the toolbox to study IL-17A in cattle and sheep.

Veterinary research

Wattegedera SR,Corripio-Miyar Y,Pang Y,Frew D,McNeilly TN,Palarea-Albaladejo J,McInnes CJ,Hope JC,Glass EJ,Entrican G

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Sat Apr 08 00:00:00 EDT 2017

Berberine exerts an anti-inflammatory role in ocular Behcet's disease.

Molecular medicine reports

Yang Y,Wang Q,Xie M,Liu P,Qi X,Liu X,Li Z

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Transcriptional signature of human pro-inflammatory T_H17 cells identifies reduced IL10 gene expression in multiple sclerosis.

Nature communications

Hu D,Notarbartolo S,Croonenborghs T,Patel B,Cialic R,Yang TH,Aschenbrenner D,Andersson KM,Gattorno M,Pham M,Kivisakk P,Pierre IV,Lee Y,Kiani K,Bokarewa M,Tjon E,Pochet N,Sallusto F,Kuchroo VK,Weiner HL

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Fri Nov 17 00:00:00 EST 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

A fully humanized IgG-like bispecific antibody for effective dual targeting of CXCR3 and CCR6.

PloS one

Robert R,Juglair L,Lim EX,Ang C,Wang CJH,Ebert G,Dolezal O,Mackay CR

25-7179 was used in Flow cytometry/Cell sorting to study whether simultaneous targeting of both CXCR3 and CCR6 with a bi-specific antibody results in decreased chemotaxis and/or specific depletion of pro-inflammatory T cell subsets.

Mon Oct 16 00:00:00 EDT 2017

Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition.

Nature medicine

Vaccari M,Gordon SN,Fourati S,Schifanella L,Liyanage NP,Cameron M,Keele BF,Shen X,Tomaras GD,Billings E,Rao M,Chung AW,Dowell KG,Bailey-Kellogg C,Brown EP,Ackerman ME,Vargas-Inchaustegui DA,Whitney S,Doster MN,Binello N,Pegu P,Montefiori DC,Foulds K,Quinn DS,Donaldson M,Liang F,Loré K,Roederer M,Koup RA,McDermott A,Ma ZM,Miller CJ,Phan TB,Forthal DN,Blackburn M,Caccuri F,Bissa M,Ferrari G,Kalyanaraman V,Ferrari MG,Thompson D,Robert-Guroff M,Ratto-Kim S,Kim JH,Michael NL,Phogat S,Barnett SW,Tartaglia J,Venzon D,Stablein DM,Alter G,Sekaly RP,Franchini G

25-7179 was used in Flow cytometry/Cell sorting to assess the efficacy of a recombinant Aventis Pasteur's canarypox vector-SIV and gp120 alum vaccine at delaying the onset of SIVmac251 in rhesus macaques.

Fri Jul 01 00:00:00 EDT 2016

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.

PLoS pathogens

Lindestam Arlehamn CS,McKinney DM,Carpenter C,Paul S,Rozot V,Makgotlho E,Gregg Y,van Rooyen M,Ernst JD,Hatherill M,Hanekom WA,Peters B,Scriba TJ,Sette A

25-7179 was used in an ELISpot assay to estimate the breadth of T cell responses for the first time in the context of an infection and human population setting.

Fri Jul 01 00:00:00 EDT 2016

A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.

PLoS pathogens

Lindestam Arlehamn CS,McKinney DM,Carpenter C,Paul S,Rozot V,Makgotlho E,Gregg Y,van Rooyen M,Ernst JD,Hatherill M,Hanekom WA,Peters B,Scriba TJ,Sette A

25-7179 was used in an ELISpot assay to estimate the breadth of T cell responses for the first time in the context of an infection and human population setting.

Fri Jul 01 00:00:00 EDT 2016

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.

Stem cell reports

Wang WB,Yen ML,Liu KJ,Hsu PJ,Lin MH,Chen PM,Sudhir PR,Chen CH,Chen CH,Sytwu HK,Yen BL

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

25-7179 was used in Flow cytometry/Cell sorting to study the role of CD4(+) T cells in latent tuberculosis infection, showing that tuberculosis antigen-specific T cells have novel multifunctional features.

Mon Sep 15 00:00:00 EDT 2014

An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis.

Immunity

Boisson B,Wang C,Pedergnana V,Wu L,Cypowyj S,Rybojad M,Belkadi A,Picard C,Abel L,Fieschi C,Puel A,Li X,Casanova JL

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Thu Oct 17 00:00:00 EDT 2013

Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.

PloS one

Zhang L,Li YG,Li YH,Qi L,Liu XG,Yuan CZ,Hu NW,Ma DX,Li ZF,Yang Q,Li W,Li JM

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2012

Gain-of-function human STAT1 mutations impair IL-17 immunity and underlie chronic mucocutaneous candidiasis.

The Journal of experimental medicine

Liu L,Okada S,Kong XF,Kreins AY,Cypowyj S,Abhyankar A,Toubiana J,Itan Y,Audry M,Nitschke P,Masson C,Toth B,Flatot J,Migaud M,Chrabieh M,Kochetkov T,Bolze A,Borghesi A,Toulon A,Hiller J,Eyerich S,Eyerich K,Gulácsy V,Chernyshova L,Chernyshov V,Bondarenko A,Grimaldo RM,Blancas-Galicia L,Beas IM,Roesler J,Magdorf K,Engelhard D,Thumerelle C,Burgel PR,Hoernes M,Drexel B,Seger R,Kusuma T,Jansson AF,Sawalle-Belohradsky J,Belohradsky B,Jouanguy E,Bustamante J,Bué M,Karin N,Wildbaum G,Bodemer C,Lortholary O,Fischer A,Blanche S,Al-Muhsen S,Reichenbach J,Kobayashi M,Rosales FE,Lozano CT,Kilic SS,Oleastro M,Etzioni A,Traidl-Hoffmann C,Renner ED,Abel L,Picard C,Maródi L,Boisson-Dupuis S,Puel A,Casanova JL

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2011

Interleukin-12 is the optimum cytokine to expand human Th17 cells in vitro.

Clinical and vaccine immunology : CVI

Nady S,Ignatz-Hoover J,Shata MT

Published figure using IL-17A monoclonal antibody (Product # 25-7179-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2009

生物信息学

蛋白别名: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A