Abnova

LIMK2 Monoclonal Antibody (2H2-E11)

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LIMK2 Antibody (H00003985-M01) in WB

Western Blot analysis of LIMK2 expression in transfected 293T cell line by LIMK2 monoclonal antibody (M01), clone 2H2-E11. Lane 1: LIMK2 transfected lysate(72.232 KDa). Lane 2: Non-transfected lysate. .

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产品信息

H00003985-M01

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

免疫细胞化学 (ICC/IF)

10-100 µg/mL

酶联免疫吸附实验 (ELISA)

10 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2H2-E11

抗原

LIMK2 (AAH13051.1, 1 a.a. approximately 686 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.34 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Product may be used with Western Blot (Transfected lysate).

Immunogen sequence: MGSYLSVPAY FTSRDLFRCS ECQDSLTNWY YEKDGKLYCP KDYWGKFGEF CHGCSLLMTG PFMVAGEFKY HPECFACMSC KVIIEDGDAY ALVQHATLYC GKCHNEVVLA PMFERLSTES VQEQLPYSVT LISMPATTEG RRGFSVSVES ACSNYATTVQ VKEVNRMHIS PNNRNAIHPG DRILEINGTP VRTLRVEEVE DAISQTSQTL QLLIEHDPVS QRLDQLRLEA RLAPHMQNAG HPHALSTLDT KENLEGTLRR RSLRRSNSIS KSPGPSSPKE PLLFSRDISR SESLRCSSSY SQQIFRPCDL IHGEVLGKGF FGQAIKVTHK ATGKVMVMKE LIRCDEETQK TFLTEVKVMR SLDHPNVLKF IGVLYKDKKL NLLTEYIEGG TLKDFLRSMD PFPWQQKVRF AKGIASGMAY LHSMCIIHRD LNSHNCLIKL DKTVVVADFG LSRLIVEERK RAPMEKATTK KRTLRKNDRK KRYTVVGNPY WMAPEMLNGK SYDETVDIFS FGIVLCEIIG QVYADPDCLP RTLDFGLNVK LFWEKFVPTD CPPAFFPLAA ICCRLEPESR APPGAAGEGP GCADDEGPVR RQGKVTIKYD PKELRKHLNL EEWILEQLTR LYDCQEEEIS ELEIDVDELL DMESDDAWAS RVKELLVDCY KPTEAFISGL LDKIRAMQKL STPQKK

靶标信息

LIMK2 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK2 has also been implicated in brain development. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。