Invitrogen

MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), PerCP-eFluor™ 710, eBioscience™

MHC Class I (H-2Kd) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Kd) Antibody (46-5957-82) in Flow

Staining of C57Bl/6 (left) or BALB/c (right) splenocytes with 0.125 µg of Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (blue histogram) or 0.125 µg of Anti-Mouse MHC Class I (H-2Kd) PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-5957-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

SF1-1.1.1

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10735380

产品详细信息

Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

Applications Reported: This SF1-1.1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis.

Communications biology

Ma D,Hernandez GA,Lefebvre AEYT,Alshetaiwi H,Blake K,Dave KR,Rauf M,Williams JW,Davis RT,Evans KT,Longworth A,Masoud MYG,Lee R,Edwards RA,Digman MA,Kessenbrock K,Lawson DA

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 46-5957-82) in Flow Cytometry

Fri Nov 05 00:00:00 EDT 2021

Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma.

Cancer cell

Dheilly E,Battistello E,Katanayeva N,Sungalee S,Michaux J,Duns G,Wehrle S,Sordet-Dessimoz J,Mina M,Racle J,Farinha P,Coukos G,Gfeller D,Mottok A,Kridel R,Correia BE,Steidl C,Bassani-Sternberg M,Ciriello G,Zoete V,Oricchio E

46-5957 was used in Flow cytometry/Cell sorting to find that follicular lymphoma patients harbor a recurrent hotspot mutation targeting tyrosine 132 (Y132D) in cathepsin S (CTSS) that enhances protein activity.

Mon May 11 00:00:00 EDT 2020

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

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Thu Feb 13 00:00:00 EST 2020

Targeting photodynamic and photothermal therapy to the endoplasmic reticulum enhances immunogenic cancer cell death.

Nature communications

Li W,Yang J,Luo L,Jiang M,Qin B,Yin H,Zhu C,Yuan X,Zhang J,Luo Z,Du Y,Li Q,Lou Y,Qiu Y,You J

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 46-5957-82) in Flow Cytometry

Fri Jul 26 00:00:00 EDT 2019

Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange.

Scientific reports

Kelton W,Waindok AC,Pesch T,Pogson M,Ford K,Parola C,Reddy ST

46-5957 was used in Flow Cytometry to demonstrate a proof-of-concept for reprogramming MHC-specificity by performing CRISPR-Cas9-assisted cassette exchange.

Tue Apr 04 00:00:00 EDT 2017

A therapeutic cancer vaccine against GL261 murine glioma.

Journal of translational medicine

Kindy MS,Yu J,Zhu H,Smith MT,Gattoni-Celli S

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 46-5957-82) in Flow Cytometry

Tue Jan 05 00:00:00 EST 2016

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 46-5957-82) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

生物信息学

蛋白别名: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MGC184092; MGC7052; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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基因别名: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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功能

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

参与通路

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development