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Invitrogen
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 615, eBioscience™
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Mouse IgG1 kappa Isotype Control (42-4714-80) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human breast carcinoma using 20 µg/mL of Mouse IgG1 Isotype Control eFluor® 615 (left) or 20 µg/mL Anti-Human CD277 (Mucin 1) eFluor® 615 (right). Nuclei are counterstained with DAPI.
产品信息
42-4714-80
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
20 µg/mL
免疫细胞化学 (ICC/IF)
1-10 µg/mL
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
P3.6.2.8.1
偶联物
激发/发射光谱
595/614 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10733160
产品详细信息
Description: The monoclonal mouse IgG1 kappa immunoglobulin is useful as an isotype control.
Applications Reported: Mouse IgG1 K Isotype Control eFluor® 615 has been reported for use in immunocytochemical (ICC) and immunohistochemical (IHC-P) staining.
Applications Tested: This Mouse IgG1, K Isotype Control eFluor® 615 has been tested by immunocytochemistry on fixed and permeabilized MDCK cells and by immunohistochemistry (IHC-P) on formalin-fixed paraffin embedded human brain tissue. Use isotype control at the same concentration as experimental antibody.
Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. A standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
