Invitrogen

PAI1 Monoclonal Antibody (1D5)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

7 篇参考文献

查看其他29个PAI1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

PAI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 11

PAI1 Antibody (MA5-17171) in IHC (P)

Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using SERPINE1 monoclonal antibody (Product # MA5-17171) followed with DAB staining.

PAI1 Antibody in Immunohistochemistry (IHC)

PAI1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

PAI1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of PAI-1 using PAI-1 Monoclonal Antibody (Product # MA5-17171) shows induction in expression of PAI-1 in A549 cells upon TGF beta treatment. {TM}

产品信息

MA5-17171

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:200-1:1,000

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1D5

抗原

Purified recombinant fragment of human SERPINE1 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2538642

产品详细信息

MA5-17171 targets SERPINE1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17171 immunogen is purified recombinant fragment of human SERPINE1 expressed in E. Coli.

MA5-17171 detects SERPINE1 which has a predicted molecular weight of approximately 45kDa.

靶标信息

PAI1 (plasminogen activator inhibitor 1) belongs to serine protease inhibitor superfamily, and is the principal inhibitor of tissue-type and urokinase-type plasminogen activators (tPA and uPA). Platelets are the main source of the circulating PAI11, but it is synthesized and secreted by many tissue and cell types, including fibroblasts, smooth muscle cells, endothelial cells, hepatocytes, and inflammatory cells. Expression of PAI11 can be regulated at the transcriptional level by many factors including growth factors, cytokines, hormones, inflammatory factors, glucose or lipid metabolites, vascular tone regulating factors, chemicals, and other environmental or physical factors. PAI1 is present at increased levels in various disease states, and has been linked to an increased occurrence of thrombosis in obesity, thrombophilia and the metabolic syndrome. Defects in PAI-1 are characterized by abnormal bleeding. PAI1 mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator, and may promote neuronal survival. Other defects in PAI1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency). Alternatively spliced transcript variants encoding different isoforms of PAI1 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PAI-1 Regulation of p53 Expression and Senescence in Type II Alveolar Epithelial Cells.

Cells

Rana T,Jiang C,Banerjee S,Yi N,Zmijewski JW,Liu G,Liu RM

Published figure using PAI1 monoclonal antibody (Product # MA5-17171) in Immunoprecipitation

Sat Aug 05 00:00:00 EDT 2023

PAI-1 Regulation of p53 Expression and Senescence in Type II Alveolar Epithelial Cells.

Cells

Rana T,Jiang C,Banerjee S,Yi N,Zmijewski JW,Liu G,Liu RM

Published figure using PAI1 monoclonal antibody (Product # MA5-17171) in Immunoprecipitation

Sat Aug 05 00:00:00 EDT 2023

Fat-1 Transgenic Mice With Augmented n3-Polyunsaturated Fatty Acids Are Protected From Liver Injury Caused by Acute-On-Chronic Ethanol Administration.

Frontiers in pharmacology

Warner J,Hardesty J,Song Y,Sun R,Deng Z,Xu R,Yin X,Zhang X,McClain C,Warner D,Kirpich I

MA5-17171 was used in Immunohistochemistry to interrogate the effects of endogenous n3-PUFA enrichment in a mouse model which recapitulates features of early human alcohol-associated hepatitis.

Thu Sep 29 00:00:00 EDT 2022

Fat-1 Transgenic Mice With Augmented n3-Polyunsaturated Fatty Acids Are Protected From Liver Injury Caused by Acute-On-Chronic Ethanol Administration.

Frontiers in pharmacology

Warner J,Hardesty J,Song Y,Sun R,Deng Z,Xu R,Yin X,Zhang X,McClain C,Warner D,Kirpich I

MA5-17171 was used in Immunohistochemistry to interrogate the effects of endogenous n3-PUFA enrichment in a mouse model which recapitulates features of early human alcohol-associated hepatitis.

Thu Sep 29 00:00:00 EDT 2022

MAPKAP Kinase-2 Drives Expression of Angiogenic Factors by Tumor-Associated Macrophages in a Model of Inflammation-Induced Colon Cancer.

Frontiers in immunology

Suarez-Lopez L,Kong YW,Sriram G,Patterson JC,Rosenberg S,Morandell S,Haigis KM,Yaffe MB

MA5-17171 was used in Immunohistochemistry to identify a role for MK2-dependent regulation of the well-known pro-angiogenesis factor CXCL-12/SDF-1 secreted by tumor associated-macrophages, in addition to MK2-dependent regulation of Serpin-E1/PAI-1 by several cell types within the tumor microenvironment.

Wed Jun 23 00:00:00 EDT 2021

MAPKAP Kinase-2 Drives Expression of Angiogenic Factors by Tumor-Associated Macrophages in a Model of Inflammation-Induced Colon Cancer.

Frontiers in immunology

Suarez-Lopez L,Kong YW,Sriram G,Patterson JC,Rosenberg S,Morandell S,Haigis KM,Yaffe MB

Wed Jun 23 00:00:00 EDT 2021

Various stress stimuli rewire the profile of liver secretome in a p53-dependent manner.

Cell death & disease

Charni-Natan M,Solomon H,Molchadsky A,Jacob-Berger A,Goldfinger N,Rotter V

Published figure using PAI1 monoclonal antibody (Product # MA5-17171) in Immunohistochemistry

Tue May 29 00:00:00 EDT 2018

Endogenous transcripts control miRNA levels and activity in mammalian cells by target-directed miRNA degradation.

Nature communications

Ghini F,Rubolino C,Climent M,Simeone I,Marzi MJ,Nicassio F

MA5-17171 was used in Western Blotting to conclude a sophisticated regulatory layer of miRNA and gene expression mediated by specific endogenous targets exists in mammalian cells.

Tue Aug 07 00:00:00 EDT 2018

Association of autotaxin and lysophosphatidic acid receptor 3 with aggressiveness of human breast carcinoma.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Popnikolov NK,Dalwadi BH,Thomas JD,Johannes GJ,Imagawa WT

MA5-17171 was used in western blot to evaluate the LPA-producing enzyme autotaxin and LPAR3 as potential therapeutic targets in breast cancer patients

Sat Dec 01 00:00:00 EST 2012

生物信息学

蛋白别名: Endothelial plasminogen activator inhibitor; nexin, plasminogen activator inhibitor type 1; PAI; plas; Plasminogen activator inhibitor 1; plasminogen activator inhibitor, type I; Plasminogen activator inhibitor-1 precursor (PAI-1) (Endothelial plasminogen activator inhibitor) (PAI); serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serine (or cysteine) proteinase inhibitor, clade E, member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; SERPINE-1

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基因别名: PAI; PAI-1; PAI1; PLANH1; SERPINE1

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UniProt ID: (Human) P05121

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Entrez Gene ID: (Human) 5054, (Mouse) 18787

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功能

protease binding serine-type endopeptidase inhibitor activity receptor binding protein binding peptidase inhibitor activity calmodulin-related protease inhibitor

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