Invitrogen

Phospho-HCLS1 (Tyr397) Recombinant Rabbit Monoclonal Antibody (HS1Y397-F12)

查看其他25个HCLS1抗体

抗体检测数据 (5)

Phospho-HCLS1 (Tyr397) Antibody in Western Blot (WB)

Phospho-HCLS1 (Tyr397) Antibody in Western Blot (WB)

图: 1 / 5

Phospho-HCLS1 (Tyr397) Antibody (MA5-37159) in WB

Western blot analysis of Phospho-HS1 (Tyr397) using Ramos cell extract. Samples were untreated or treated with 300 nM Thapsigargin (30 min), and incubated in monoclonal Phospho-HS1 (Tyr397) antibody (Product # MA5-37159) with a dilution of 0.01 µg/mL.

Phospho-HCLS1 (Tyr397) Antibody in Western Blot (WB)

Phospho-HCLS1 (Tyr397) Antibody in Western Blot (WB)

Phospho-HCLS1 (Tyr397) Antibody in Flow Cytometry (Flow)

Phospho-HCLS1 (Tyr397) Antibody in Flow Cytometry (Flow)

Phospho-HCLS1 (Tyr397) Antibody in Flow Cytometry (Flow)

Product Image Gallery

Phospho-HCLS1 (Tyr397) Antibody in Western Blot (WB) — Western blot analysis of Phospho-HS1 (Tyr397) using Ramos cell extract. Samples were untreated or treated with 300 nM Thapsigargin (30 min), and incubated in monoclonal Phospho-HS1 (Tyr397) antibody (Product # MA5-37159) with a dilution of 0.01 µg/mL.

Phospho-HCLS1 (Tyr397) Antibody in Western Blot (WB) — Western blot analysis of Phospho-HS1 (Tyr397) using Ramos cell extract. Samples were untreated or treated with 300 nM thapsigargin (30 min), and incubated in monoclonal Phospho-HS1 (Tyr397) antibody (Product # MA5-37159) with a dilution of 3 ng/mL.

Phospho-HCLS1 (Tyr397) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-HS1 (Tyr397) using Ramos cells. Samples were incubated in monoclonal Phospho-HS1 (Tyr397) antibody (Product # MA5-37159) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with pervandadate (green), untreated and blocked with phospho-peptide (black), treated and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), treated and blocked with non-phospho peptide (purple).

Phospho-HCLS1 (Tyr397) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-HS1 (Tyr397) using Ramos cells. Samples were incubated in monoclonal Phospho-HS1 (Tyr397) antibody (Product # MA5-37159) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with pervanadate (green).

Phospho-HCLS1 (Tyr397) Antibody in Flow Cytometry (Flow) — Flow cytometry of Phospho-HS1 (Tyr397) using mouse cells. Samples were incubated in monoclonal Phospho-HS1 (Tyr397) antibody (Product # MA5-37159) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (blue), control (red), stimulated (green).

产品信息

MA5-37159

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

-

流式细胞分析 (Flow)

1-1,000 ng/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

HS1Y397-F12

抗原

A synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human phospho HS1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 50% glycerol, 0.10% BSA

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2897093

靶标信息

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; LckBP1; p75

查看更多收起

基因别名: AW213261; CTTNL; HCLS1; HS1; lckBP1; p75

查看更多收起

UniProt ID: (Human) P14317, (Mouse) P49710

查看更多收起

Entrez Gene ID: (Human) 3059, (Mouse) 15163

查看更多收起

RNA polymerase II transcription factor binding actin binding protein binding SH3 domain binding protein kinase binding protein complex binding actin or actin-binding cytoskeletal protein cytoskeletal protein non-motor actin binding protein

参与通路

negative regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated positive regulation of cell proliferation response to hormone positive regulation of phosphatidylinositol 3-kinase signaling actin filament polymerization erythrocyte differentiation regulation of actin filament polymerization positive regulation of granulocyte differentiation positive regulation of peptidyl-serine phosphorylation intracellular signal transduction positive regulation of tyrosine phosphorylation of STAT protein positive regulation of transcription factor import into nucleus positive regulation of macrophage differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein kinase B signaling cellular response to cytokine stimulus negative regulation of leukocyte apoptotic process positive regulation of actin cytoskeleton reorganization