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Phospho-LIMK2 (Ser283) Antibody (PA5-104926) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-LIMK2 (Ser283) in human gastric cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-LIMK2 (Ser283) polyclonal antibody (Product # PA5-104926) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
产品信息
PA5-104926
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human LIMK2(Accession P53671), corresponding to amino acid residues around phosphorylated Ser283.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816399
产品详细信息
Antibody detects endogenous levels of LIMK2 only when phosphorylated at Serine 283.
靶标信息
LIMK2 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK2 has also been implicated in brain development. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 2.7.11.1; kinase LIMK2; LIM domain kinase 2; LIM kinase 2; LIM motif-containing protein kinase 2; LIMK; LIMK 2; LIMK-2; LIMK1; LINhp-2; OTTHUMP00000199483
基因别名: Limk-2; LIMK2; Limk2b; Link2
UniProt ID: (Human) P53671, (Mouse) O54785, (Rat) P53670
Entrez Gene ID: (Human) 3985, (Mouse) 16886, (Rat) 29524
