Invitrogen

TCF4 Polyclonal Antibody

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TCF4 Antibody (PA5-77796) in WB

Western blot analysis of TCF4 in mouse liver lysate using 15 µg of protein. Sample was prepared with 5% milk and TBST, incubated with TCF4 polyclonal antibody (Product # PA5-77796) using a dilution of 1:1000 (2 hours at RT) and followed by a Goat Anti-Rabbit HRP secondary antibody at a dilution of 1:3000 (1 hour at RT). Samples are arranged as follows: Lane 1: molecular weight ladder, Lane 2: Mouse liver lysate.

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产品信息

PA5-77796

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:50

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2736688

产品详细信息

A 1:1,000 dilution of PA5-77796 was sufficient for detection of TCF4 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects 71 kDa, 45 kDa. 45 kDa band represents a different isoform .

靶标信息

TCF4 is transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG')-a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Defects in this gene are a cause of Pitt-Hopkins syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: bHLHb19; Class A helix-loop-helix transcription factor ME2; Class B basic helix-loop-helix protein 19; E2 2; Immunoglobulin transcription factor 2; immunoglobulin transcription factor-2; ITF 2; ITF-2; MGC149723; MGC149724; MITF-2; SEF 2; SEF-2; SEF2 1; SEF2 1A; SEF2 1B; SL3-3 enhancer factor 2; TCF 4; TCF-4; Transcription factor 4; Transcription factor 4 (Immunoglobulin transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer factor 2) (SEF-2) (Class A helix-loop-helix transcription factor ME2); transcription factor 4, isoform C; transcription factor 4, isoform D; transcription factor 4, isoform E; transcription factor 4, isoform L; transcription factor 4, isoform M; transcription factor 4, isoform R

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基因别名: 5730422P05Rik; ASP-I2; BHLHB19; E2-2; E2.2; FECD3; ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4; TCF4; TFE

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UniProt ID: (Human) P15884, (Mouse) Q60722

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Entrez Gene ID: (Human) 6925, (Mouse) 21413

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