Custom Chicken Polyclonal Antibody Production Protocols

Immunization and egg-collection procedures for custom chicken polyclonal antibody production

Our comprehensive array of custom antibody services includes a customizable option for chicken immunization, egg-collection, and titer analysis for chicken antibody production, plus several extension possibilities.

Custom Antibody Request

Highlights

Custom chicken antibodies—a reliable protocol for immunizing chickens, collecting eggs, testing bleeds, reporting titer results, and delivering eggs
Full-service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification (see Custom Polyclonal Antibodies pages for details)

2-chicken 80-day chicken immunization protocol

This chicken immunization schedule uses two White Leghorn chickens. Animals are housed individually in separate cages, injections are subcutaneous (SQ) as emulsions in Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA); alternative adjuvants can be used upon request. Customizations to the protocol are available. For example, you can choose the number of chickens needed and you can adjust the length of your protocol beyond the 80-day immunization schedule to meet your research goals. Please contact us to discuss the options in detail. Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before day 0. Protocols days are approximate (± 2 days).

Procedure	Protocol day	Description
Control serum collection	Day 0	Pre-immune bleed (~2mL per chicken)
Primary injection	Day 1	Immunize with 0.25 mg antigen in CFA, SQ 4 sites
1st booster	Day 14	Boost with 0.10 mg antigen in IFA, SQ 4 sites
Egg collection	Day 21	Collect eggs for one week
2nd booster	Day 42	Boost with 0.10 mg antigen in IFA, SQ 4 sites
Egg collection	Day 48	Collect eggs for one week
Serum collection	Day 56	Test-bleed (~1-2 mL per chicken)
ELISA and shipping	Day 65	ELISA titration (results available online); Verify disposition of chickens; decide to continue or terminate
Instruction due date	Day 80	If no animal instructions are received, per diem charges will apply
Deliverables (Day 65): 32 vials 2 vials: 2 mL per vial pre-immune sera 2 vials: 1-2 mL test bleed post-inject sera (Day 56) 28 vials: post-inject eggs (2 week egg collection)