AP conjugated mouse IgG (H+L) cross-adsorbed secondary antibody in western blotting

Alkaline phosphatase (AP)-conjugated secondary antibodies are commonly used in enzyme driven applications, such as western blotting, ELISA, immuno-histochemistry, and others. AP-conjugated secondary antibodies use alkaline phosphatase substrates mainly for chemiluminescent or colorimetric detection. Navigate this page to find our menu of AP-conjugated secondary antibodies along with recommended substrates for your western blotting, ELISA, or immuno-histochemistry experiments.

Search all AP-Conjugated Secondary Antibodies

AP-conjugated secondary antibodies by application

Our offerings for high-activity AP-conjugated secondary antibodies are sorted below in the tabular format by application. Included with the selection tables for AP-linked secondary antibodies are recommended substrates for each application by detection method, including chemiluminescent or colorimetric.
 

Table 1. Alkaline phosphatase secondary antibodies for western blotting

Target speciesHost
GoatRabbitMouseDonkeyChickenSheep
Anti-MouseAP-conjugated goat anti-mouseAP-conjugated rabbit anti-mouse—AP-conjugated donkey anti-mouse——
Anti-HumanAP-conjugated goat anti-humanAP-conjugated rabbit anti-humanAP-conjugated mouse anti-human——AP-conjugated sheep anti-human
Anti-RabbitAP-conjugated goat anti-rabbit——AP-conjugated donkey anti-rabbitAP-conjugated chicken anti-rabbit—
Anti-RatAP-conjugated goat anti-ratAP-conjugated rabbit anti-rat—AP-conjugated donkey anti-ratAP-conjugated chicken anti-ratAP-conjugated sheep anti-rat
Anti-Goat—AP-conjugated rabbit anti-goat—AP-conjugated donkey anti-goatAP-conjugated chicken anti-goat—
Anti-BovineAP-conjugated goat anti-bovineAP-conjugated rabbit anti-bovine————
Anti-ChickenAP-conjugated goat anti-chickenAP-conjugated rabbit anti-chicken————
Anti-HamsterAP-conjugated goat anti-hamsterAP-conjugated rabbit anti-hamster————
Anti-Guinea PigAP-conjugated goat anti-guinea pigAP-conjugated rabbit anti-guinea pig————
Anti-Sheep—AP-conjugated rabbit anti-sheep—AP-conjugated donkey anti-sheep——
Anti-DogAP-conjugated goat anti-dog—————
Anti-CamelidAP-conjugated goat anti-llamaAP-conjugated rabbit anti-llama————
Anti-DonkeyAP-conjugated goat anti-donkeyAP-conjugated rabbit anti-donkey————


Recommend AP substrates for western blotting

1-Step™ NBT/BCIP Substrate SolutionNovex AP Chemiluminescent Substrate (CDP-Star)
1-Step NBT/BCIP Substrate for AP-conjugated secondary antibodies for western blottingNovex AP Chemiluminescent Substrate for AP-conjugated secondary antibodies for western blotting
Readout: ColorimetricReadout: Chemiluminescent
Low background, ready-to-use formatHigh sensitivity, ready-to-use format
Recommended antibody dilution: 
1°: 1:500
2°: 1:5,000 to 1:50,000		Recommended antibody dilution: 
1°: 1:500 to 5,000
2°: 1:2,000 to 1:10,000
Order hereOrder here

Table 2. Alkaline phosphatase secondary antibodies for ELISA applications

Target speciesHost
GoatRabbitMouseDonkeyChickenSheep
Anti-MouseAP-conjugated goat anti-mouseAP-conjugated rabbit anti-mouse—AP-conjugated donkey anti-mouse——
Anti-HumanAP-conjugated goat anti-humanAP-conjugated rabbit anti-humanAP-conjugated mouse anti-human——AP-conjugated sheep anti-human
Anti-RabbitAP-conjugated goat anti-rabbit——AP-conjugated donkey anti-rabbitAP-conjugated chicken anti-rabbit—
Anti-RatAP-conjugated goat anti-ratAP-conjugated rabbit anti-rat—AP-conjugated donkey anti-ratAP-conjugated chicken anti-ratAP-conjugated sheep anti-rat
Anti-Goat—AP-conjugated rabbit anti-goat—AP-conjugated donkey anti-goatAP-conjugated chicken anti-goat—
Anti-BovineAP-conjugated goat anti-bovineAP-conjugated rabbit anti-bovine————
Anti-ChickenAP-conjugated goat anti-chickenAP-conjugated rabbit anti-chicken————
Anti-HamsterAP-conjugated goat anti-hamsterAP-conjugated rabbit anti-hamster————
Anti-Guinea PigAP-conjugated goat anti-guinea pigAP-conjugated rabbit anti-guinea pig————
Anti-Sheep———AP-conjugated donkey anti-sheep——
Anti-DogAP-conjugated goat anti-dog—————
Anti-CamelidAP-conjugate goat anti-llamaAP-conjugated rabbit anti-llama————
Anti-DonkeyAP-conjugated goat anti-donkeyAP-conjugated rabbit anti-donkey————


Recommend AP substrates for ELISA

1-Step PNPP Substrate SolutionCDP-Star Substrate (0.25 mM Ready-To-Use)
1-Step PNPP Substrate for AP-conjugated secondary antibodies for ELISACDP Star Substrate for AP-conjugated secondary antibodies for ELISA
Readout: ColorimetricReadout: Chemiluminescent
Order hereOrder here

Table 3. Alkaline phosphatase secondary antibodies for IHC

Target speciesHost
GoatRabbitMouseDonkeyChickenSheep
Anti-MouseAP-conjugated goat anti-mouseAP-conjugated rabbit anti-mouse—AP-conjugated donkey anti-mouse——
Anti-HumanAP-conjugated goat anti-humanAP-conjugated rabbit anti-humanAP-conjugated mouse anti-human——AP-conjugated sheep anti-human
Anti-RabbitAP-conjugated goat anti-rabbit——AP-conjugated donkey anti-rabbitAP-conjugated chicken anti-rabbit—
Anti-RatAP-conjugated goat anti-ratAP-conjugated rabbit anti-rat—AP-conjugated donkey anti-ratAP-conjugated chicken anti-ratAP-conjugated sheep anti-rat
Anti-Goat—AP-conjugated rabbit anti-goat—AP-conjugated donkey anti-goatAP-conjugated chicken anti-goat—
Anti-BovineAP-conjugated goat anti-bovineAP-conjugated rabbit anti-bovine————
Anti-ChickenAP-conjugated goat anti-chicken—————
Anti-HamsterAP conjugated goat anti-hamsterAP conjugated rabbit anti-hamster————
Anti-Guinea PigAP-conjugated goat anti-guinea pigAP-conjugated rabbit anti-guinea pig————
Anti-Sheep———AP-conjugated donkey anti-sheep——
Anti-DogAP-conjugated goat anti-dog—————
Anti-CamelidAP-conjugated goat anti-llamaAP-conjugated rabbit anti-llama————
Anti-DonkeyAP-conjugated goat anti-donkeyAP-conjugated rabbit anti-donkey————

Recommend AP substrates for IHC

1-Step™ NBT/BCIP Substrate Solution
1-Step NBT/BCIP Substrate for AP-conjugated secondary antibodies for IHC
Readout: Colorimetric
Low background, ready-to-use format
Recommended antibody dilution: 
1°: 1:500
2°: 1:5,000 to 1:50,000
Order here
Western blotting

Table 1. Alkaline phosphatase secondary antibodies for western blotting

Target speciesHost
GoatRabbitMouseDonkeyChickenSheep
Anti-MouseAP-conjugated goat anti-mouseAP-conjugated rabbit anti-mouse—AP-conjugated donkey anti-mouse——
Anti-HumanAP-conjugated goat anti-humanAP-conjugated rabbit anti-humanAP-conjugated mouse anti-human——AP-conjugated sheep anti-human
Anti-RabbitAP-conjugated goat anti-rabbit——AP-conjugated donkey anti-rabbitAP-conjugated chicken anti-rabbit—
Anti-RatAP-conjugated goat anti-ratAP-conjugated rabbit anti-rat—AP-conjugated donkey anti-ratAP-conjugated chicken anti-ratAP-conjugated sheep anti-rat
Anti-Goat—AP-conjugated rabbit anti-goat—AP-conjugated donkey anti-goatAP-conjugated chicken anti-goat—
Anti-BovineAP-conjugated goat anti-bovineAP-conjugated rabbit anti-bovine————
Anti-ChickenAP-conjugated goat anti-chickenAP-conjugated rabbit anti-chicken————
Anti-HamsterAP-conjugated goat anti-hamsterAP-conjugated rabbit anti-hamster————
Anti-Guinea PigAP-conjugated goat anti-guinea pigAP-conjugated rabbit anti-guinea pig————
Anti-Sheep—AP-conjugated rabbit anti-sheep—AP-conjugated donkey anti-sheep——
Anti-DogAP-conjugated goat anti-dog—————
Anti-CamelidAP-conjugated goat anti-llamaAP-conjugated rabbit anti-llama————
Anti-DonkeyAP-conjugated goat anti-donkeyAP-conjugated rabbit anti-donkey————


Recommend AP substrates for western blotting

1-Step™ NBT/BCIP Substrate SolutionNovex AP Chemiluminescent Substrate (CDP-Star)
1-Step NBT/BCIP Substrate for AP-conjugated secondary antibodies for western blottingNovex AP Chemiluminescent Substrate for AP-conjugated secondary antibodies for western blotting
Readout: ColorimetricReadout: Chemiluminescent
Low background, ready-to-use formatHigh sensitivity, ready-to-use format
Recommended antibody dilution: 
1°: 1:500
2°: 1:5,000 to 1:50,000		Recommended antibody dilution: 
1°: 1:500 to 5,000
2°: 1:2,000 to 1:10,000
Order hereOrder here
ELISA

Table 2. Alkaline phosphatase secondary antibodies for ELISA applications

Target speciesHost
GoatRabbitMouseDonkeyChickenSheep
Anti-MouseAP-conjugated goat anti-mouseAP-conjugated rabbit anti-mouse—AP-conjugated donkey anti-mouse——
Anti-HumanAP-conjugated goat anti-humanAP-conjugated rabbit anti-humanAP-conjugated mouse anti-human——AP-conjugated sheep anti-human
Anti-RabbitAP-conjugated goat anti-rabbit——AP-conjugated donkey anti-rabbitAP-conjugated chicken anti-rabbit—
Anti-RatAP-conjugated goat anti-ratAP-conjugated rabbit anti-rat—AP-conjugated donkey anti-ratAP-conjugated chicken anti-ratAP-conjugated sheep anti-rat
Anti-Goat—AP-conjugated rabbit anti-goat—AP-conjugated donkey anti-goatAP-conjugated chicken anti-goat—
Anti-BovineAP-conjugated goat anti-bovineAP-conjugated rabbit anti-bovine————
Anti-ChickenAP-conjugated goat anti-chickenAP-conjugated rabbit anti-chicken————
Anti-HamsterAP-conjugated goat anti-hamsterAP-conjugated rabbit anti-hamster————
Anti-Guinea PigAP-conjugated goat anti-guinea pigAP-conjugated rabbit anti-guinea pig————
Anti-Sheep———AP-conjugated donkey anti-sheep——
Anti-DogAP-conjugated goat anti-dog—————
Anti-CamelidAP-conjugate goat anti-llamaAP-conjugated rabbit anti-llama————
Anti-DonkeyAP-conjugated goat anti-donkeyAP-conjugated rabbit anti-donkey————


Recommend AP substrates for ELISA

1-Step PNPP Substrate SolutionCDP-Star Substrate (0.25 mM Ready-To-Use)
1-Step PNPP Substrate for AP-conjugated secondary antibodies for ELISACDP Star Substrate for AP-conjugated secondary antibodies for ELISA
Readout: ColorimetricReadout: Chemiluminescent
Order hereOrder here
Immunohistochemistry

Table 3. Alkaline phosphatase secondary antibodies for IHC

Target speciesHost
GoatRabbitMouseDonkeyChickenSheep
Anti-MouseAP-conjugated goat anti-mouseAP-conjugated rabbit anti-mouse—AP-conjugated donkey anti-mouse——
Anti-HumanAP-conjugated goat anti-humanAP-conjugated rabbit anti-humanAP-conjugated mouse anti-human——AP-conjugated sheep anti-human
Anti-RabbitAP-conjugated goat anti-rabbit——AP-conjugated donkey anti-rabbitAP-conjugated chicken anti-rabbit—
Anti-RatAP-conjugated goat anti-ratAP-conjugated rabbit anti-rat—AP-conjugated donkey anti-ratAP-conjugated chicken anti-ratAP-conjugated sheep anti-rat
Anti-Goat—AP-conjugated rabbit anti-goat—AP-conjugated donkey anti-goatAP-conjugated chicken anti-goat—
Anti-BovineAP-conjugated goat anti-bovineAP-conjugated rabbit anti-bovine————
Anti-ChickenAP-conjugated goat anti-chicken—————
Anti-HamsterAP conjugated goat anti-hamsterAP conjugated rabbit anti-hamster————
Anti-Guinea PigAP-conjugated goat anti-guinea pigAP-conjugated rabbit anti-guinea pig————
Anti-Sheep———AP-conjugated donkey anti-sheep——
Anti-DogAP-conjugated goat anti-dog—————
Anti-CamelidAP-conjugated goat anti-llamaAP-conjugated rabbit anti-llama————
Anti-DonkeyAP-conjugated goat anti-donkeyAP-conjugated rabbit anti-donkey————

Recommend AP substrates for IHC

1-Step™ NBT/BCIP Substrate Solution
1-Step NBT/BCIP Substrate for AP-conjugated secondary antibodies for IHC
Readout: Colorimetric
Low background, ready-to-use format
Recommended antibody dilution: 
1°: 1:500
2°: 1:5,000 to 1:50,000
Order here


Fluorescent substrates for signal amplification of AP conjugates

Endogenous phosphatase activity in zebra brain visualized with ELF Endogenous Phosphatase Kit

Figure 1. Endogenous phosphatase activity in zebra brain visualized with ELF Endogenous Phosphatase Kit
(Cat. No. E6601).

ELF signal amplification is based on ELF 97 phosphate, a substrate for both alkaline phosphatase and acid phosphatase. Upon cleavage this substrate yields a bright yellow-green fluorescence with excellent photostability. The ELF 97 signal is more photostable than signals achieved using either direct or indirect detection with fluorescein conjugates. ELF 97 is well suited for immunolabeling and can be detected with imaging instrumentation that is equipped with a UV light excitation source.

Order here

Do you need help deciding on which substrate to use? Please see our substrate selection tool
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