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| Highest purity for molecular assays | Affordable & pure, flow compatible cells | Extreme purity & physiological quality | Untouched - most physiological relevant cells | ||||
|---|---|---|---|---|---|---|---|
| Isolation Method | Depletion or positive isolation | Positive isolation | Positive isolation | Negative isolation | |||
| Starting samples | Any sample (whole blood, MNC, bone marrow) | Any sample (whole blood, MNC, bone marrow) | Any sample (whole blood, MNC, bone marrow) | MNC* | |||
| # MNC processed (mL whole blood processed) | 1-2 x 109 MNC
200 mL whole blood | 2 x 109 MNC
80 mL whole blood | 2 x 109 MNC
400 mL whole blood | 1 x 109 cells | |||
| Purity | Highest
98-100% | Higher
95-99% | Highest
98-100% | High
90-95% | |||
| Yield | Highest
95-100% | High
70-85% | Standard
50-70% | Standard
50-70% | |||
| Viability | >95% | >95% | >95% | >95% | |||
| Isolation Time | ~30 min | ~60 min | ~90 min | ~50 min | |||
| Isolation Temp. | Cold | Cold | Release at room temp. | Cold | |||
| Applications | Depletion of unwanted CD8+ T cells or positive isolation for molecular e.g. prior to proteins & nucleic acids | Cellular assays (cell culture, cell activation/proliferation, cytokine studies)
Molecular isolation of proteins & nucleic acids | Cellular assays (cell culture, cell activation/proliferation, cytokine studies)
Translational research Molecular isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells) | |||
| Bead-free Target Cells | No | Yes (but antibody present) | Yes (and antibody-free) | Yes | |||
| Compatible with
Flow Cytometry /Sorting | No | Yes | Yes | Yes | |||
| Shelf Life from Production Date | 3 years | 2 years | 3 years | 2 years | |||
| Required Materials | Magnets | Magnets | Magnets | Magnets | |||
| Order Now | Order Now | Order Now | Order Now |
*MNC - Mononuclear cells
THIS IS FOR HUMAN RESEARCH—NOT FOR MOUSE