经典多色流式实验方案 | Thermo Fisher Scientific

多重验证的流式抗体方案

从2010年起，“高级多色流式配色方案(OMIP)”说的是经过充分测试和验证，用于检测细胞状态或反应的多色表型的流式抗体和试剂。在Wiley Online Library 的Cytometry Part A 杂志中，高级多色流式配色方案(OMIP)适用于流式实验中，还可用于成像流式、荧光显微镜和其他多色荧光检测方法。

阅读BioProbes 74 中高级多色流式配色方案 (OMIP)。

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Cytometry Part A 杂志中OMIP 系列文章

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OMIP ID	OMIP 文献标题	目的细胞类型
OMIP-001	Quality and phenotype of Ag-responsive human T cells	人 T 细胞
OMIP-002	Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes	人 T 细胞
OMIP-003	Phenotypic analysis of human memory B cells	人 B 细胞
OMIP-004	In-depth characterization of human T regulatory cells	人 T 细胞
OMIP-005	Quality and phenotype of antigen-responsive rhesus macaque T cells	恒河猴 T 细胞
OMIP-006	Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry	人 T 细胞
OMIP-007	A phenotypic analysis of human natural killer cells	人 NK 细胞
OMIP-008	Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells	人 T 细胞
OMIP-009	Characterization of antigen-specific human T cells	人 T 细胞
OMIP-010	A new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples	人造血细胞
OMIP-011	Characterization of circulating endothelial cells (CECs) in peripheral blood	人内皮细胞
OMIP-012	Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice	人源化小鼠中的人白细胞
OMIP-013	Differentiation of human T-cells	人 T 细胞
OMIP-014	Validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining	人 T 细胞
OMIP-015	Human regulatory and activated T-cells without intracellular staining	人 T 细胞
OMIP-016	Characterization of antigen-responsive macaque and human T-cells	食蟹猴或人 T 细胞
OMIP-017	Human CD4⁺ helper T-cell subsets including follicular helper cells	人 T 细胞
OMIP-018	Chemokine receptor expression on human T helper cells	人 T 细胞
OMIP-019	Quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors	人 T 细胞、NK 细胞
OMIP-020	Phenotypic characterization of human γδ T-cells by multicolor flow cytometry	人 T 细胞
OMIP-021	Simultaneous quantification of human conventional and innate-like T-cell subsets	人 T 细胞
OMIP-022	Comprehensive assessment of antigen-specific human T-cell functionality and memory	人 T 细胞
OMIP-023	10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes	人白细胞
OMIP-024	Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples	人白细胞
OMIP-025	Evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining	人 T 细胞、NK 细胞
OMIP-026	Phenotypic analysis of B and plasma cells in rhesus macaques	恒河猴 B 细胞、浆细胞
OMIP-027	Functional analysis of human natural killer cells	人 NK 细胞
OMIP-028	Activation panel for rhesus macaque NK cell subsets	恒河猴 NK 细胞
OMIP-029	Human NK-cell phenotypization	人 NK 细胞
OMIP-030	Characterization of human T cell subsets via surface markers	人 T 细胞
OMIP-031	Immunologic checkpoint expression on murine effector and memory T-cell subsets	小鼠 T 细胞
OMIP-032	Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland	小鼠白细胞
OMIP-033	A comprehensive single step staining protocol for human T- and B-cell subsets	人 T 细胞、B 细胞
OMIP-034	Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies	人白细胞
OMIP-035	Functional analysis of natural killer cell subsets in macaques	恒河猴 NK 细胞
OMIP-036	Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets	人 T 细胞
OMIP-037	16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets	人淋巴细胞
OMIP-038	Innate immune assessment with a 14 color flow cytometry panel	人淋巴细胞
OMIP-039	Detection and analysis of human adaptive NKG2C+ natural killer cells	NK 细胞、淋巴细胞
OMIP-040	Optimized gating of human prostate cellular subpopulations	人白细胞、上皮细胞和基质细胞
OMIP-041	Optimized multicolor immunofluorescence panel rat microglial staining protocol	大鼠原代小胶质细胞
OMIP-042	21‐color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood	人外周血单个核细胞 (PBMCs)
OMIP-043	Identification of human antibody secreting cell subsets	人新鲜或冻存的外周血单个核细胞 (PBMCs)、骨髓、脾脏或扁桃体细胞
OMIP-044	28-color immunophenotyping of the human dendritic cell compartment	人树突细胞
OMIP-045	Characterizing human head and neck tumors and cancer cell lines with mass cytometry	癌细胞株
OMIP-046	Characterization of invariant T cell subset activation in humans	人外周血单个核细胞 (PBMCs)
OMIP-047	High‐Dimensional phenotypic characterization of B cells	人外周血单个核细胞 (PBMCs)
OMIP-048	Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry	脾脏和淋巴结来源的鼠原代淋巴细胞
OMIP-049	Analysis of Human Myelopoiesis and Myeloid Neoplasms	人髓细胞
OMIP-050	A 28‐color/30‐parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules	人外周血单个核细胞 (PBMCs)
OMIP-051	28-color flow cytometry panel to characterize B cells and myeloid cells	人 B 细胞
OMIP-052	An 18‐Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques	恒河猴外周血单个核细胞 (PBMCs)
OMIP-053	Identification, Classification, and Isolation of Major FoxP3 Expressing Human CD4+ Treg Subsets	人外周血单个核细胞 (PBMCs)
OMIP-054	Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry	小鼠 T 淋巴细胞
OMIP-055	Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral Blood	人淋巴细胞
OMIP-056	Evaluation of Human Conventional T Cells, Donor‐Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine Staining	冻存的外周血单个核细胞 (PBMCs)
OMIP-057	Mouse γδ T‐Cell Development Characterized by a 14 Color Flow Cytometry Panel	小鼠胸腺细胞
OMIP-058	30‐Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T Cells	人外周血单个核细胞 (PBMCs)
OMIP-059	Identification of Mouse Hematopoietic Stem and Progenitor Cells with Simultaneous Detection of CD45.1/2 and Controllable Green Fluorescent Protein Expression by a Single Staining Panel	小鼠造血干细胞
OMIP-060	30‐Parameter Flow Cytometry Panel to Assess T Cell Effector Functions and Regulatory T Cells	人外周血单个核细胞 (PBMCs)
OMIP-061	20‐Color Flow Cytometry Panel for High‐Dimensional Characterization of Murine Antigen‐Presenting Cells	小鼠树突状细胞
OMIP-062	A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples	人外周血单个核细胞 (PBMCs)
OMIP-063	28‐Color Flow Cytometry Panel for Broad Human Immunophenotyping	人外周血单个核细胞 (PBMCs)
OMIP-064	A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T Cells	人外周血单个核细胞 (PBMCs)
OMIP-065	Dog Immunophenotyping and T‐Cell Activity Evaluation with a 14‐Color Panel	犬外周血单个核细胞 (PBMCs)
OMIP-066	Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral Blood	人外周血单个核细胞 (PBMCs)
OMIP-067	28‐Color Flow Cytometry Panel to Evaluate Human T‐Cell Phenotype and Function	人外周血单个核细胞 (PBMCs)
OMIP-068	High‐Dimensional Characterization of Global and Antigen‐Specific B Cells in Chronic Infection	人外周血单个核细胞 (PBMCs)
OMIP-069	Forty-Color Full Spectrum Flow CytometryPanel for Deep Immunophenotyping of Major CellSubsets in Human Peripheral Blood	人白细胞
OMIP-070	NKp46‐Based 27‐Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor Tissues	人肿瘤细胞
OMIP-071	A 31‐Parameter Flow Cytometry Panel for In‐Depth Immunophenotyping of Human T‐Cell Subsets Using Surface Markers	人 T 细胞
OMIP-072	A 15‐color panel for immunophenotypic identification, quantification, and characterization of leukemic stem cells in children with acute myeloid leukemia	白血病干细胞
OMIP-073	Analysis of human thymocyte development with a 14‐color flow cytometry panel	人胸腺细胞
OMIP-074	Phenotypic analysis of IgG and IgA subclasses on human B cells	人外周血单个核细胞 (PBMCs)
OMIP-075	A 22-color panel for the measurement of antigen-specific T-cell responses in human and nonhuman primates	人和非人灵长类外周血单个核细胞 (PBMCs)
OMIP-076	High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsets	小鼠 T 细胞
OMIP-077	Definition of all principal human leukocyte populations using a broadly applicable 14-color panel	人白细胞
OMIP-078	A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cells	人 T 细胞
OMIP-079	Cell cycle of CD4+ and CD8+ naïve/memory T cell subsets, and of Treg cells from mouse spleen	小鼠脾细胞
OMIP-080	29-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantation	人外周血单个核细胞 (PBMCs)
OMIP-081	A new 21-monoclonal antibody 10-color panel for diagnostic polychromatic immunophenotyping	人骨髓和外周血