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了解反转录、反转录酶和 cDNA 应用的基础知识
Find everything you need to know about reverse transcription and reverse transcriptases. Review our educational resources intended for new and experienced researchers alike. Begin your search with the basics of reverse transcription, and further your knowledge with more topics covering cDNA synthesis, reverse transcriptases troubleshooting tips, and cDNA applications in molecular biology studies.
Learn about the roles of reverse transcription in biological systems and in molecular biology research.
Examine essential factors for cDNA synthesis, such as RNA purity, the reverse transcriptase selected, primers used, reaction components, and reaction conditions.
Find out how polymerase activity, RNase H activity, thermostability, and processivity of a reverse transcriptase can impact its functions as a molecular biology tool.
Discover uses of reverse transcription in RT-PCR, cDNA cloning, RACE, gene expression microarrays, and RNA sequencing.
Review troubleshooting tips on reverse transcription with a focus on RT-PCR issues.
逆转录视频
五步攻克反转录挑战，出色进行 RT-qPCR
如何在 10 分钟内完成反转录
使用纯度不佳的 RNA 进行 cDNA 合成
如何通过一步法 RT-PCR 获得高特异性结果
一步法 PCR ：实验原理以及两阶段热启动如何提高反应特异性
如何实现更快、更简单、更高效的 cDNA 合成
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Stylesheet for Classic Wide Template adjustments
Overcoming RT-qPCR challengesOvercoming RT-qPCR challenges
Reverse Transcriptase Reaction in 10 minutes — Enzyme ProcessivitySuperScript IV reverse transcriptase is the most robust reverse transcriptase with increased processivity and more resistant to reaction inhibitors such as guanidinum, heparin and salt as compared to wild type reverse transcriptases.
Inhibitors in Reverse Transcriptase ReactionSuperScript IV reverse transcriptase is the most robust reverse transcriptase with increased processivity and more resistant to reaction inhibitors such as guanidinum and salt as compared to wild type reverse transcriptases.
Understanding Reverse Transcriptase — Effects on Ct valueThe reverse transcription step is one of the greatest sources of variation in RT-qPCR. With SuperScript IV reverse transcriptase, the Ct value can be reduced by as much as 8. This enzyme outperforms wild type reverse transcriptases with better sens
Simplified RT — Reverse Transcription AnimationLearn how reverse transcription works in a fun animated video and how to select the right reverse transcriptases and primers for your experiment.
仅供科研使用，不可用于诊断目的。