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以下教程将向您展示,如何方便地使用我们屡获殊荣的BLOCK-iT™ RNAi Designer,针对您的合成RNAi与基于载体的RNAi产物设计和购买定制序列。
1. On the Note: For online ordering, be sure you have your customer login. If not, please register. | ||
2. On the BLOCK-iT™ RNAi Designer page, select your Target Design Option (product type). 3. Input target sequence information: accession number or nucleotide sequence. 4. Select design options: Start with default. Increase G/C range if necessary. Finally, include UTRs as a last option. 5. Click "RNAi Design" button. | ||
6. Select designs using check boxes. Recommendation: Select top 2 or 3 star rankings to take advantage of guarantees for Stealth™ RNAi, siRNA, and miR RNAi vectors. Note: Star rankings are relative—all designs displayed are likely to be effective. 7. Click "Continue" button. | ||
8. Check boxes to order scrambled negative controls, if desired. 9. Choose "Order Online" or "Email/Save Order". The "Order Online" option requires customers to login (or register) to complete their order. The "Email/Save Order" option allows customers to export the designed siRNAs to a spreadsheet, which can be sent to genomicorders@invitrogen.com or |