SOLiD next-generation sequencing helps move your research closer to the discovery of biologically relevant outcomes by enabling excellent accuracy, flexibility, and ease of use. The 5500 W Series Genetic Analyzers enable direct amplification of libraries on the FlowChip for a simplified workflow, to help reduce cost per sample, and time to results. Use the tabs below to find the kits, reagents, and accessories to support your next-generation sequencing applications.  

Step-by-step guide to kits and reagents for SOLiD next-generation sequencing

DNA

PureLink™ Genomic DNA Mini Kit 
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.

RNA

PureLink™ RNA Mini Kit 
Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types. 

DNA

SequalPrep™ System
Robust target enrichment by long PCR (5-20kb) for enhanced sequencing of targeted regions, followed by one-step amplicon purification and input equalization in a convenient plate for accurate target representation.

Cot-1DNA Blocking Reagent
Increase hybridization sensitivity on array-based exon capture platforms by blocking repetitive sequences.

RNA

RiboMinus™ RNA-Seq Kits
Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.

Epigenome

Magnify™ Chromatin Immunoprecipitation System
Faster and more reproducible solution for Chromatin Immunoprecipitation (ChIP) with the ability to start with lower cell numbers to preserve precious samples.

MethylMiner™ Methylated DNA Conversion Kit
Enrichment & differential fractionation of dsDNA by CpG methylation density.  Increased sensitivity over antibody methods.

Cells-to-CpG™ Bisulfite Conversion Kit
Fast & easy conversion of unmethylated cytosines into uracils while methylated cytosines remain unchanged, starting directly from cells, tissue, blood, FFPE, or purified DNA.

DNA Libraries

SOLiD Fragment Library Construction
Fully optimized complete set of reagents, columns, and size selection gels to generate fragment libraries for use on SOLiD System.

SOLiD Mate-Paired Library Construction
Fully optimized, validated, complete set of reagents to construct mate-paired libraries for use on SOLiD System.

SOLiD ChIP-Seq Kits
Complete & optimized system for genome wide Chromatin Immunoprecipitation (ChIP) analysis on SOLiD.  (additional purchase of Magnify™ ChIP System not required)

E-Gel™ SizeSelect™ Gels
Rapid size separation and recovery system for library construction.


RNA Libraries

SOLiD Total RNA-Seq Kit
Fully optimized, validated kit to construct strand-specific libraries for small RNA and whole transcriptome interrogation on SOLiD System.

SOLiD SAGE™ Kit
Fully optimized reagents for tag-based quantification of transcription expression profiles on SOLiD System.

SOLiD Library TaqMan Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD library for optimal input in emulsion PCR (ePCR).

Ambion ERCC Spike-In Mixes
Measure sensitivity and quantitate differential gene expression between RNA experiments, including those run on an Next Generation Sequencing instrument.

DNA Libraries

SOLiD Fragment Library Barcoding Kits
Unique barcode sequences for SOLiD fragment libraries to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

RNA Libraries

SOLiD RNA Barcoding Kits
Unique barcode sequences for small RNA and whole transcriptome libraries generated with SOLiD Total RNA-Seq Kit or SOLiD SAGE™ libraries  to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

Step 1 : DNA & RNA Extraction
DNA

PureLink™ Genomic DNA Mini Kit 
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.

RNA

PureLink™ RNA Mini Kit 
Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types. 

Step 2 : Enrichment
DNA

SequalPrep™ System
Robust target enrichment by long PCR (5-20kb) for enhanced sequencing of targeted regions, followed by one-step amplicon purification and input equalization in a convenient plate for accurate target representation.

Cot-1DNA Blocking Reagent
Increase hybridization sensitivity on array-based exon capture platforms by blocking repetitive sequences.

RNA

RiboMinus™ RNA-Seq Kits
Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.

Epigenome

Magnify™ Chromatin Immunoprecipitation System
Faster and more reproducible solution for Chromatin Immunoprecipitation (ChIP) with the ability to start with lower cell numbers to preserve precious samples.

MethylMiner™ Methylated DNA Conversion Kit
Enrichment & differential fractionation of dsDNA by CpG methylation density.  Increased sensitivity over antibody methods.

Cells-to-CpG™ Bisulfite Conversion Kit
Fast & easy conversion of unmethylated cytosines into uracils while methylated cytosines remain unchanged, starting directly from cells, tissue, blood, FFPE, or purified DNA.

Step 3 : Library Generation
DNA Libraries

SOLiD Fragment Library Construction
Fully optimized complete set of reagents, columns, and size selection gels to generate fragment libraries for use on SOLiD System.

SOLiD Mate-Paired Library Construction
Fully optimized, validated, complete set of reagents to construct mate-paired libraries for use on SOLiD System.

SOLiD ChIP-Seq Kits
Complete & optimized system for genome wide Chromatin Immunoprecipitation (ChIP) analysis on SOLiD.  (additional purchase of Magnify™ ChIP System not required)

E-Gel™ SizeSelect™ Gels
Rapid size separation and recovery system for library construction.


RNA Libraries

SOLiD Total RNA-Seq Kit
Fully optimized, validated kit to construct strand-specific libraries for small RNA and whole transcriptome interrogation on SOLiD System.

SOLiD SAGE™ Kit
Fully optimized reagents for tag-based quantification of transcription expression profiles on SOLiD System.

Step 4 : Library Quantitiation

SOLiD Library TaqMan Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD library for optimal input in emulsion PCR (ePCR).

Ambion ERCC Spike-In Mixes
Measure sensitivity and quantitate differential gene expression between RNA experiments, including those run on an Next Generation Sequencing instrument.

Step 5 : Library Barcoding
DNA Libraries

SOLiD Fragment Library Barcoding Kits
Unique barcode sequences for SOLiD fragment libraries to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

RNA Libraries

SOLiD RNA Barcoding Kits
Unique barcode sequences for small RNA and whole transcriptome libraries generated with SOLiD Total RNA-Seq Kit or SOLiD SAGE™ libraries  to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

Kits and reagents
DNA

PureLink™ Genomic DNA Mini Kit 
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.

RNA

PureLink™ RNA Mini Kit 
Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types. 

DNA

SequalPrep™ System
Robust target enrichment by long PCR (5-20kb) for enhanced sequencing of targeted regions, followed by one-step amplicon purification and input equalization in a convenient plate for accurate target representation.

Cot-1DNA Blocking Reagent
Increase hybridization sensitivity on array-based exon capture platforms by blocking repetitive sequences.

RNA

RiboMinus™ RNA-Seq Kits
Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.

Epigenome

Magnify™ Chromatin Immunoprecipitation System
Faster and more reproducible solution for Chromatin Immunoprecipitation (ChIP) with the ability to start with lower cell numbers to preserve precious samples.

MethylMiner™ Methylated DNA Conversion Kit
Enrichment & differential fractionation of dsDNA by CpG methylation density.  Increased sensitivity over antibody methods.

Cells-to-CpG™ Bisulfite Conversion Kit
Fast & easy conversion of unmethylated cytosines into uracils while methylated cytosines remain unchanged, starting directly from cells, tissue, blood, FFPE, or purified DNA.

DNA Libraries

SOLiD Fragment Library Construction
Fully optimized complete set of reagents, columns, and size selection gels to generate fragment libraries for use on SOLiD System.

SOLiD Mate-Paired Library Construction
Fully optimized, validated, complete set of reagents to construct mate-paired libraries for use on SOLiD System.

SOLiD ChIP-Seq Kits
Complete & optimized system for genome wide Chromatin Immunoprecipitation (ChIP) analysis on SOLiD.  (additional purchase of Magnify™ ChIP System not required)

E-Gel™ SizeSelect™ Gels
Rapid size separation and recovery system for library construction.


RNA Libraries

SOLiD Total RNA-Seq Kit
Fully optimized, validated kit to construct strand-specific libraries for small RNA and whole transcriptome interrogation on SOLiD System.

SOLiD SAGE™ Kit
Fully optimized reagents for tag-based quantification of transcription expression profiles on SOLiD System.

SOLiD Library TaqMan Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD library for optimal input in emulsion PCR (ePCR).

Ambion ERCC Spike-In Mixes
Measure sensitivity and quantitate differential gene expression between RNA experiments, including those run on an Next Generation Sequencing instrument.

DNA Libraries

SOLiD Fragment Library Barcoding Kits
Unique barcode sequences for SOLiD fragment libraries to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

RNA Libraries

SOLiD RNA Barcoding Kits
Unique barcode sequences for small RNA and whole transcriptome libraries generated with SOLiD Total RNA-Seq Kit or SOLiD SAGE™ libraries  to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

Step 1 : DNA & RNA Extraction
DNA

PureLink™ Genomic DNA Mini Kit 
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.

RNA

PureLink™ RNA Mini Kit 
Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types. 

Step 2 : Enrichment
DNA

SequalPrep™ System
Robust target enrichment by long PCR (5-20kb) for enhanced sequencing of targeted regions, followed by one-step amplicon purification and input equalization in a convenient plate for accurate target representation.

Cot-1DNA Blocking Reagent
Increase hybridization sensitivity on array-based exon capture platforms by blocking repetitive sequences.

RNA

RiboMinus™ RNA-Seq Kits
Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.

Epigenome

Magnify™ Chromatin Immunoprecipitation System
Faster and more reproducible solution for Chromatin Immunoprecipitation (ChIP) with the ability to start with lower cell numbers to preserve precious samples.

MethylMiner™ Methylated DNA Conversion Kit
Enrichment & differential fractionation of dsDNA by CpG methylation density.  Increased sensitivity over antibody methods.

Cells-to-CpG™ Bisulfite Conversion Kit
Fast & easy conversion of unmethylated cytosines into uracils while methylated cytosines remain unchanged, starting directly from cells, tissue, blood, FFPE, or purified DNA.

Step 3 : Library Generation
DNA Libraries

SOLiD Fragment Library Construction
Fully optimized complete set of reagents, columns, and size selection gels to generate fragment libraries for use on SOLiD System.

SOLiD Mate-Paired Library Construction
Fully optimized, validated, complete set of reagents to construct mate-paired libraries for use on SOLiD System.

SOLiD ChIP-Seq Kits
Complete & optimized system for genome wide Chromatin Immunoprecipitation (ChIP) analysis on SOLiD.  (additional purchase of Magnify™ ChIP System not required)

E-Gel™ SizeSelect™ Gels
Rapid size separation and recovery system for library construction.


RNA Libraries

SOLiD Total RNA-Seq Kit
Fully optimized, validated kit to construct strand-specific libraries for small RNA and whole transcriptome interrogation on SOLiD System.

SOLiD SAGE™ Kit
Fully optimized reagents for tag-based quantification of transcription expression profiles on SOLiD System.

Step 4 : Library Quantitiation

SOLiD Library TaqMan Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD library for optimal input in emulsion PCR (ePCR).

Ambion ERCC Spike-In Mixes
Measure sensitivity and quantitate differential gene expression between RNA experiments, including those run on an Next Generation Sequencing instrument.

Step 5 : Library Barcoding
DNA Libraries

SOLiD Fragment Library Barcoding Kits
Unique barcode sequences for SOLiD fragment libraries to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

RNA Libraries

SOLiD RNA Barcoding Kits
Unique barcode sequences for small RNA and whole transcriptome libraries generated with SOLiD Total RNA-Seq Kit or SOLiD SAGE™ libraries  to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.

Accessories

仅供科研使用，不可用于诊断目的。