BPAE imaging using CellROX® Deep Red Reagent (Cat. No. C10422)

Bovine pulmonary artery endothelial (BPAE) cells were plated in a 96-well plate. BPAE cells were treated with or without 100 µM menadione for 1hour at 37°C. 100 µM superoxide scavenger, MnTBAP, was added to some of the control and menadione-treated wells for the last 30 minutes of incubation. The cells were then stained with 5 µM CellROX® Deep Red Reagent and Hoechst 33324 by adding the probe to the complete medium and incubating the cells at 37°C for 30 minutes. The cells were then washed with PBS and imaged.

Bovine pulmonary artery endothelial (BPAE) cells were plated in a 96-well plate. BPAE cells were treated with or without 100 µM menadione for 1hour at 37°C. 100 µM superoxide scavenger, MnTBAP, was added to some of the control and menadione-treated wells for the last 30 minutes of incubation. The cells were then stained with 5 µM CellROX® Deep Red Reagent and Hoechst 33324 by adding the probe to the complete medium and incubating the cells at 37°C for 30 minutes. The cells were then washed with PBS and imaged.

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货号 产品名称 规格 单价 (CNY) 数量
C10422 CellROX™ Deep Red Reagent, for oxidative stress detection Each
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飞享价

8,138.00

截止至: 2025/12/31

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