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Invitrogen

ALDH1A3 Polyclonal Antibody

Name: ALDH1A3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA529188
Price: 6273.0 CNY

高级验证

5个已发表图片

6 篇参考文献

查看其他8个ALDH1A3抗体

说明书

实验方案

技术支持

货号 PA5-29188

CNY 6,273.00

100 µL

下单后预计有货时间 14-Apr-2025

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说明书

实验方案

技术支持

Application

ALDH1A3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 17

ALDH1A3 Antibody (PA5-29188) in ICC/IF

Immunofluorescence analysis of ALDH1A3 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ALDH1A3 Polyclonal Antibody (Product # PA5-29188) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More

ALDH1A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALDH1A3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

PA5-29188

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 126 and 423 of Human ALDH1A3

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2546664

产品详细信息

Recommended positive controls: A431, Huh-7, U87-MG.

Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (83%), Chicken (88%), Rhesus Monkey (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ALDH1A3 are aldehyde dehydrogenase isozymes which are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by the ALDH1A3 gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Repurposing Disulfiram for Targeting of Glioblastoma Stem Cells: An In Vitro Study.

Biomolecules

Zirjacks L,Stransky N,Klumpp L,Prause L,Eckert F,Zips D,Schleicher S,Handgretinger R,Huber SM,Ganser K

Published figure using ALDH1A3 polyclonal antibody (Product # PA5-29188) in Western Blot

Thu Oct 21 00:00:00 UTC 2021

ALDH1A3 Segregated Expression and Nucleus-Associated Proteasomal Degradation Are Common Traits of Glioblastoma Stem Cells.

Biomedicines

Fauß J,Sprang B,Leukel P,Sommer C,Nikolova T,Ringel F,Kim EL

PA5-29188 was used in Immunocytochemistry-immunofluorescence to support the association of ALDH1A3 with glioblastoma stem cells and provide evidence for the regulation of ALDH1A3 activities at the level of protein turnover.

Wed Dec 22 00:00:00 UTC 2021

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

PA5-29188 was used in Immunocytochemistry-immunoflourescence to report that PKCλ expression was enriched in basal-like breast cancer, among breast cancer subtypes, and was correlated with ALDH1A3 expression (p = 0.016, χ2-test).

Thu Sep 10 00:00:00 UTC 2020

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

Thu Sep 10 00:00:00 UTC 2020

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

Thu Sep 10 00:00:00 UTC 2020

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

Thu Sep 10 00:00:00 UTC 2020

Serum erythropoietin levels, breast cancer and breast cancer-initiating cells.

Breast cancer research : BCR

Bhat K,Sandler K,Duhachek-Muggy S,Alli C,Cheng F,Moatamed NA,Magyar CE,Du L,Li G,McCloskey S,Vlashi E,Pajonk F

PA5-29188 was used in Immunohistochemistry to conclude that physiologically slow-rising serum erythropoietin levels in response to tumor-related or chemotherapy-induced anemia, as opposed to large doses of recombinant erythropoietin, do not increase the pool of breast cancer-initiating cells.

Wed Jan 30 00:00:00 UTC 2019

Multi-omics profiling reveals key signaling pathways in ovarian cancer controlled by STAT3.

Theranostics

Lu T,Bankhead A,Ljungman M,Neamati N

PA5-29188 was used in Western Blotting to provide a rich, multi-faceted summary of the molecular mechanisms impacted by STAT3 deletion and provide new insight for STAT3's potential as a therapeutic target in ovarian cancer.

Wed Sep 09 00:00:00 UTC 2020

生物信息学

蛋白别名: acetaldehyde dehydrogenase 6; aldehyde dehydrogenase 1 family, member A3; Aldehyde dehydrogenase 6; Aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase family 1, subfamily A3; aldehyde dehydrogenase-6; ALDH1A3; ALDHA1A3; RALDH-3; ralDH3; Retinaldehyde dehydrogenase 3

基因别名: ALDH1A3; ALDH1A6; ALDH6; MCOP8; RALDH3; V1

UniProt ID: (Human) P47895, (Mouse) Q9JHW9, (Rat) Q8K4D8

Entrez Gene ID: (Human) 220, (Mouse) 56847, (Rat) 266603

功能

retinal dehydrogenase activity aldehyde dehydrogenase (NAD) activity aldehyde dehydrogenase [NAD(P)+] activity protein homodimerization activity thyroid hormone binding NAD+ binding 3-chloroallyl aldehyde dehydrogenase activity oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor dehydrogenase oxidoreductase metabolite interconversion enzyme

参与通路

optic cup morphogenesis involved in camera-type eye development retinoic acid biosynthetic process locomotory behavior nucleus accumbens development inner ear morphogenesis retinol metabolic process retinoic acid metabolic process retinal metabolic process positive regulation of apoptotic process embryonic eye morphogenesis neuromuscular process controlling balance oxidation-reduction process righting reflex olfactory pit development face development cellular aldehyde metabolic process metabolic process embryonic camera-type eye development positive regulation of retinoic acid receptor signaling pathway kidney development pituitary gland development response to drug

ALDH1A3 Polyclonal Antibody

ALDH1A3 Antibody (PA5-29188) in ICC/IF

产品信息

PA5-29188

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

ALDH1A3 Polyclonal Antibody

ALDH1A3 Antibody (PA5-29188) in ICC/IF

产品信息

PA5-29188

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID