Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
SHC Antibody (44829M) in WB
Whole cell lysates of serum starved HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5) PC12 (6), CMT 93 (7), Neuro 2A (8) and 3T3 (9) tumor cells (approximately 20,000 cells per lane) were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer, then incubated with mouse anti-Shc/p66 (N-term) antibody (Catalog no. 44829M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antibody and signals were detected using ... View More
产品信息
44829M
产品规格
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
24E4
抗原
Peptide (N-terminal sequence) conjugated to hemocyanin.
偶联物
形式
Liquid
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Dry ice
RRID
AB_2533773
靶标信息
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
