Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), FITC, eBioscience™

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

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CD71 (Transferrin Receptor) Antibody (11-0711-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.25 µg of Anti-Mouse CD71 (Transferrin Receptor) FITC (right). Cells in the lymphocyte gate were used for analysis.

CD71 (Transferrin Receptor) Antibody in Western Blot (WB)

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产品信息

11-0711-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看30篇已发表文章 30篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Fish, Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

R17217 (RI7 217.1.4)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465124

产品详细信息

Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains.

Applications Reported: The R17217 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R17217 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors.

Cancer communications (London, England)

Hosaka K,Wang C,Zhang S,Lv X,Seki T,Zhang Y,Jing X,Wu J,Du Q,He X,Fan Y,Li X,Kondo M,Yoshihara M,Qian H,Shi L,Zhu P,Xu Y,Yang Y,Cheng T,Cao Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2023

A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biology.

Life science alliance

Diaz-Vegas A,Norris DM,Jall-Rogg S,Cooke KC,Conway OJ,Shun-Shion AS,Duan X,Potter M,van Gerwen J,Baird HJ,Humphrey SJ,James DE,Fazakerley DJ,Burchfield JG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Western Blot

Sun Jan 01 00:00:00 EST 2023

Adiponectin receptors sustain haematopoietic stem cells throughout adulthood by protecting them from inflammation.

Nature cell biology

Meacham CE,Jeffery EC,Burgess RJ,Sivakumar CD,Arora MA,Stanley AM,Colby EM,Crane GM,Zhao Z,Morrison SJ

11-0711-82 was used in Flow cytometry/Cell sorting to find that adiponectin receptors are non-cell-autonomously required in haematopoietic cells to promote HSC quiescence and self-renewal.

Sun May 01 00:00:00 EDT 2022

NSMF promotes the replication stress-induced DNA damage response for genome maintenance.

Nucleic acids research

Ju MK,Shin KJ,Lee JR,Khim KW,A Lee E,Ra JS,Kim BG,Jo HS,Yoon JH,Kim TM,Myung K,Choi JH,Kim H,Chae YC

11-0711 was used in Flow cytometry/Cell sorting to identify the N-methyl-D-aspartate receptor synaptonuclear signaling and neuronal migration factor (NSMF) as a key replication stress response factor that is important for ataxia telangiectasia and Rad3-related protein (ATR) activation.

Fri Jun 04 00:00:00 EDT 2021

NSMF promotes the replication stress-induced DNA damage response for genome maintenance.

Nucleic acids research

Ju MK,Shin KJ,Lee JR,Khim KW,A Lee E,Ra JS,Kim BG,Jo HS,Yoon JH,Kim TM,Myung K,Choi JH,Kim H,Chae YC

Fri Jun 04 00:00:00 EDT 2021

Engineered red blood cells carrying PCSK9 inhibitors persistently lower LDL and prevent obesity.

PloS one

Deshycka R,Sudaryo V,Huang NJ,Xie Y,Smeding LY,Choi MK,Ploegh HL,Lodish HF,Pishesha N

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Fri Dec 24 00:00:00 EST 2021

Tandem Deubiquitination and Acetylation of SPRTN Promotes DNA-Protein Crosslink Repair and Protects against Aging.

Molecular cell

Huang J,Zhou Q,Gao M,Nowsheen S,Zhao F,Kim W,Zhu Q,Kojima Y,Yin P,Zhang Y,Guo G,Tu X,Deng M,Luo K,Qin B,Machida Y,Lou Z

11-0711-82 was used in Flow Cytometry to study the regulation and consequences of SPRTN deubiquitination. To show that two sequential post-translational modifications (PTMs) regulate SPRTN chromatin accessibility to repair DNA-protein crosslinks and maintain genomic stability and a healthy lifespan.

Thu Sep 03 00:00:00 EDT 2020

Evaluating the Role of the Steroid and Xenobiotic Receptor (SXR/PXR) in PCB-153 Metabolism and Protection against Associated Adverse Effects during Perinatal and Chronic Exposure in Mice.

Environmental health perspectives

Egusquiza RJ,Ambrosio ME,Wang SG,Kay KM,Zhang C,Lehmler HJ,Blumberg B

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

miR‑210 enhances mesenchymal stem cell‑modulated neural precursor cell migration.

Molecular medicine reports

Wang F,Zhu J,Zheng J,Duan W,Zhou Z

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal.

Cell reports

Hidalgo San Jose L,Sunshine MJ,Dillingham CH,Chua BA,Kruta M,Hong Y,Hatters DM,Signer RAJ

11-0711 was used in Flow cytometry/Cell sorting to show that hematopoietic stem cells (HSCs) are dependent on low protein synthesis to maintain proteostasis, which promotes their self-renewal.

Tue Jan 07 00:00:00 EST 2020

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

m⁶A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment.

Cell reports

Cheng Y,Luo H,Izzo F,Pickering BF,Nguyen D,Myers R,Schurer A,Gourkanti S,Brüning JC,Vu LP,Jaffrey SR,Landau DA,Kharas MG

11-0711-82 was used in Flow cytometry/Cell sorting to indicate that RNA methylation controls symmetric commitment and cell identity of hematopoietic stem cells and may provide a general mechanism for how stem cells regulate differentiation fate choice.

Tue Aug 13 00:00:00 EDT 2019

Loss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms.

The Journal of clinical investigation

Zhao B,Mei Y,Cao L,Zhang J,Sumagin R,Yang J,Gao J,Schipma MJ,Wang Y,Thorsheim C,Zhao L,Stalker T,Stein B,Wen QJ,Crispino JD,Abrams CS,Ji P

11-0711 was used in Flow cytometry/Cell sorting to identify pleckstrin-2 as an effector of the JAK2/STAT5 pathway and a key factor in the pathogenesis of JAK2V617F-induced myeloproliferative neoplasms.

Tue Jan 02 00:00:00 EST 2018

TGF-β-Induced Quiescence Mediates Chemoresistance of Tumor-Propagating Cells in Squamous Cell Carcinoma.

Cell stem cell

Brown JA,Yonekubo Y,Hanson N,Sastre-Perona A,Basin A,Rytlewski JA,Dolgalev I,Meehan S,Tsirigos A,Beronja S,Schober M

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Thu Nov 02 00:00:00 EDT 2017

Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation.

Nucleic acids research

Zhang Y,Zhang J,An W,Wan Y,Ma S,Yin J,Li X,Gao J,Yuan W,Guo Y,Engel JD,Shi L,Cheng T,Zhu X

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Wed Jan 25 00:00:00 EST 2017

LCN2 overexpression in bone enhances the hematopoietic compartment via modulation of the bone marrow microenvironment.

Journal of cellular physiology

Costa D,Principi E,Lazzarini E,Descalzi F,Cancedda R,Castagnola P,Tavella S

11071182 was used in flow cytometry to examine the function of lipocalin-2 in bone tissue

Wed Nov 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.

Nature communications

Pasricha SR,Lim PJ,Duarte TL,Casu C,Oosterhuis D,Mleczko-Sanecka K,Suciu M,Da Silva AR,Al-Hourani K,Arezes J,McHugh K,Gooding S,Frost JN,Wray K,Santos A,Porto G,Repapi E,Gray N,Draper SJ,Ashley N,Soilleux E,Olinga P,Muckenthaler MU,Hughes JR,Rivella S,Milne TA,Armitage AE,Drakesmith H

11-0711 was used in Flow cytometry/Cell sorting to study the epigenetic regulation of hepcidin expression and the effect on systemic iron levels.

Fri Sep 01 00:00:00 EDT 2017

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.

Nature communications

11-0711 was used in Flow cytometry/Cell sorting to study the epigenetic regulation of hepcidin expression and the effect on systemic iron levels.

Fri Sep 01 00:00:00 EDT 2017

The mouse Char10 locus regulates severity of pyruvate kinase deficiency and susceptibility to malaria.

PloS one

Laroque A,Min-Oo G,Tam M,Ponka P,Stevenson MM,Gros P

11-0711 was used in Flow cytometry/Cell sorting to study the mechanistic basis of the Char10 effect.

Tue Sep 12 00:00:00 EDT 2017

The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.

Molecular and cellular biology

Ghanem LR,Kromer A,Silverman IM,Chatterji P,Traxler E,Penzo-Mendez A,Weiss MJ,Stanger BZ,Liebhaber SA

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Fri Jan 15 00:00:00 EST 2016

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Molecular medicine reports

Hu Y,Zhang Y,Tian K,Xun C,Wang S,Lv D

11-0711 was used in Flow cytometry/Cell sorting to suggest that manipulation of the ERK and AKT signalling pathway may be associated with the differentiation of transfected BMSCs.

Fri Jan 01 00:00:00 EST 2016

Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity.

PloS one

Jonas BA,Johnson C,Gratzinger D,Majeti R

11-0711 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics.

Wed Jul 26 00:00:00 EDT 2017

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Genome-wide association study follow-up identifies cyclin A2 as a regulator of the transition through cytokinesis during terminal erythropoiesis.

American journal of hematology

Ludwig LS,Cho H,Wakabayashi A,Eng JC,Ulirsch JC,Fleming MD,Lodish HF,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

A Systems Approach Identifies Essential FOXO3 Functions at Key Steps of Terminal Erythropoiesis.

PLoS genetics

Liang R,Campreciós G,Kou Y,McGrath K,Nowak R,Catherman S,Bigarella CL,Rimmelé P,Zhang X,Gnanapragasam MN,Bieker JJ,Papatsenko D,Ma'ayan A,Bresnick E,Fowler V,Palis J,Ghaffari S

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0711-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals.

Nature immunology

Salmond RJ,Brownlie RJ,Morrison VL,Zamoyska R

11-0711 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity.

Mon Sep 01 00:00:00 EDT 2014

Inactivation of Rb and E2f8 synergizes to trigger stressed DNA replication during erythroid terminal differentiation.

Molecular and cellular biology

Ghazaryan S,Sy C,Hu T,An X,Mohandas N,Fu H,Aladjem MI,Chang VT,Opavsky R,Wu L

11-0711 was used in Flow cytometry/Cell sorting to show that during erythroid terminal differentiation, Rb promotes DNA replication and cell cycle progression.

Fri Aug 01 00:00:00 EDT 2014

HIF-1α is a protective factor in conditional PHD2-deficient mice suffering from severe HIF-2α-induced excessive erythropoiesis.

Blood

Franke K,Kalucka J,Mamlouk S,Singh RP,Muschter A,Weidemann A,Iyengar V,Jahn S,Wieczorek K,Geiger K,Muders M,Sykes AM,Poitz DM,Ripich T,Otto T,Bergmann S,Breier G,Baretton G,Fong GH,Greaves DR,Bornstein S,Chavakis T,Fandrey J,Gassmann M,Wielockx B

11-0711 was used in Flow cytometry/Cell sorting to study the regulatory effects of oxygen-sensitive prolyl hydroxylases on the process of erythropoiesis.

Thu Feb 21 00:00:00 EST 2013

Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.

Blood

Hu T,Ghazaryan S,Sy C,Wiedmeyer C,Chang V,Wu L

11-0711 was used in Flow cytometry/Cell sorting to reveal the specificity of E2F8 in mediating Rb function in erythropoiesis.

Thu May 10 00:00:00 EDT 2012

Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.

Blood

Hu T,Ghazaryan S,Sy C,Wiedmeyer C,Chang V,Wu L

11-0711 was used in Flow cytometry/Cell sorting to reveal the specificity of E2F8 in mediating Rb function in erythropoiesis.

Thu May 10 00:00:00 EDT 2012

Enhanced clathrin-dependent endocytosis in the absence of calnexin.

PloS one

Li HD,Liu WX,Michalak M

11-0711 was used in Flow cytometry/Cell sorting to investigate the role of the C-terminal, cytoplasmic portion of calnexin in clathrin-dependent endocytosis.