Invitrogen

SIGLEC H Monoclonal Antibody (eBio440c), Functional Grade, eBioscience™

SIGLEC H Antibody in Flow Cytometry (Flow)

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SIGLEC H Antibody (16-0333-82) in Flow

Cell reports 2020 - Figure 1. Presence of pDCs in Naive Corneas and Alterations in Their Density upon Sterile Inflammation and Acute HSV-1 Keratitis (A and B) Flow cytometric analysis of pooled corneal single-cell suspensions of naive mice (n = 15-20 corneas) and 3 days post-thermal cautery (n = 4-8 corneas). (A) Flow cytometry density plots illustrating PDCA-1 + CD45R/B220 + cells among CD45 + cells in naive and inflamed corn... View More

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产品信息

16-0333-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

2 µg/test

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功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio440c

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10548966

产品详细信息

Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio440c antibody has been tested by blocking staining of the direct conjugate of eBio440c. This can be used at less than or equal to 2.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

It is recommended that direct conjugates such as Product # 11-0333-82 be used rather than purified eBio440c antibody when performing flow cytometric analysis.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

仅用于科研。不用于诊断过程。未经明确授权不得转售。