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APC-eFluor 780 染料
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| 2 | 633 | 780/60 | 756 | 785 | (缓冲液中) 1. | 流式细胞仪 |
Invitrogen APC-eFluor 780 染料是一种近红外荧光染料，设计用于多色流式细胞术的高性能串联荧光团。其设计用于与配备红色激光的流式细胞仪配合使用，发射峰为 780 nm。APC-eFluor 780 与 APC-Alexa Fluor 750 具有相似的光谱特性，使用时无需更改仪器配置，您可以轻松地将 APC-eFluor 780 添加到多色面板中。
我们提供与流式细胞仪中的初级抗体结合使用的 APC-eFluor 780 染料。
- 与APC-H7 串联偶联物相比，灵敏度相当或更高
- 经甲醛固定或暴露于环境光下后性能稳定
- 与现有荧光染料使用相同的滤光片组
使用流式细胞仪对 APC-eFluor 780 染料和 APC-H7 染料进行比较。用 CD8a 单克隆抗体（53-6.7）、APC-eFluor 780（蓝线）或 CD8a 单克隆抗体与 APC-H7 偶联（红线）对小鼠脾细胞进行染色。
APC-eFluor 780的稳定性分析。用 CD8a 单克隆抗体（RPA-T8）、APC-eFluor 780 对人类外周血单核细胞（PBMC）进行染色，并立即在流式细胞仪上进行分析（红线）。然后将样品分成两部分，一部分暴露在环境光下 6 小时（蓝线），另一部分在室温下用 2% 的甲醛固定 30 分钟（绿线），并再次进行分析。
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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