Qdot 565 spectra excitation shown as dotted line and emission shown as solid green-yellow histogram
5350, 488, 525574/26300564(in buffer) 5microscopy, flow cytometry

Invitrogen Qdot 565 probe is an excellent orange-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~565 nm, Qdot 565 probe can be excited using 350, 488, or 525 nm laser lines or a standard TRITC filter. Optimum imaging results with brighter signals are achieved with a Qdot 565 filter or light cube.

Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also well-suited for multiplexed western blots.

We offer Qdot 565 probe conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection. In addition, reactive dye forms and antibody labeling kits are available to allow you to generate your own antibody conjugates or probes.

Qdot Label Conjugates for Cell and Tissue Staining    Search Qdot 565 secondary antibodies

Experimental data using Qdot 565 probe

Immunofluorescence analysis of Qdot 565 secondary antibody. A549 cells were stained with alpha Tubulin Rat Monoclonal Antibody (YL1/2). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL of Rat primary antibody for 3 hours at room temperature. F(ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, Qdot 565 was used at 1:1000 dilution in PBS containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI. F-actin was stained with Alexa Fluor 488 Phalloidin, 1:300 (Panel c: green). Panel d represents the composite image. No non-specific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

Additional resources

Flow cytometry protocols handbook

Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.

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