Invitrogen

CD4 Monoclonal Antibody (S3.5), PE

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

16 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 7

CD4 Antibody (MHCD0404) in Flow

Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with Alexa Fluor® 700 Zenon® labeling reagent. An antibody complex was made by using the Zenon® Alexa Fluor® 700 Mouse IgG1 Labeling Kit (Product # Z-25011) and mouse anti human CD4. Human mononuclear cells were stained with the antibody complex and analyzed by flow cytometry using a 633 nm laser.

Product Image Gallery

CD4 Antibody — Figure 7 Endocytosis was necessary in the process of DRibbles-inflammasome response. ( a ) CypHer5E-labeled DRibbles and lysotraker Green-labeled lysosome were collocated in THP-1 cells after 6 h incubation, and the nucleus were labeled with DAPI. ( b ) The endocytosis frequency of DRibbles by THP-1 cells was measured by FACS. DCs were pre-treated with ExoA (1 mu M), Cytochalasin-D (Cyto-D, 1 mu g/ml), and NH 4 Cl (20 mu M) for 30 min, then incubated with CypHer5E stained DRibbles (100 mu g/ml) overnight. ( c ) Primed THP-1 cells were pre-treated with cytochalasin-D, ExoA, and NH 4 Cl for 1 h, then stimulated with ATP/LPS (5 mM)/(10 ng/ml) and DRibbles (100 mu g/ml) overnight. The supernatant IL-1 beta was detected by ELISA. ( d ) The frequency of IFN- gamma + CD8 + , IFN- gamma + CD4 + T cells induced by pp65 DRibbles at indicated doses were measured by ICS, and ( e ) pp65 protein (10 mu g/ml) and pp65 DRibbles (10 mu g/ml). Results are shown as the means+S.D. and representative of two or more independent experiments. PBMC is from 3 donors {TM}

产品信息

MHCD0404

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看12篇已发表文章 12篇已发表文章

其他PubMed (Misc)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA, sucrose

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10376142

产品详细信息

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

This antibody may be used, in combination with other indicators, for the diagnosis or prognosis of immunodeficiency diseases, including hypogammaglobulinemia, severe combined immunodeficiency (SCID), and the acquired immunodeficiency disease (AIDS).

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD4 monoclonal antibody (Product # MHCD0404) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Published figure using CD4 monoclonal antibody (Product # MHCD0404) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

Effects of Desflurane and Sevoflurane anesthesia on regulatory T cells in patients undergoing living donor kidney transplantation: a randomized intervention trial.

BMC anesthesiology

Chutipongtanate A,Prukviwat S,Pongsakul N,Srisala S,Kamanee N,Arpornsujaritkun N,Gesprasert G,Apiwattanakul N,Hongeng S,Ittichaikulthol W,Sumethkul V,Chutipongtanate S

MHCD0404 was used in Flow cytometry/Cell sorting to investigate effects of desflurane and sevoflurane anesthesia on peripheral blood Tregs induction in patients undergoing living donor kidney transplantation.

Thu Aug 27 00:00:00 EDT 2020

Secretory High-Mobility Group Box 1 Protein Affects Regulatory T Cell Differentiation in Neuroblastoma Microenvironment In Vitro.

Journal of oncology

Vanichapol T,Chiangjong W,Panachan J,Anurathapan U,Chutipongtanate S,Hongeng S

MHCD0404 was used in Flow cytometry/Cell sorting to elucidate the role of the neuroblastoma microenvironment in promoting regulatory T cell phenotype.

Thu Oct 05 00:00:00 EDT 2023

Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF-IRF4 transcription factor complex.

Nature immunology

Iwata A,Durai V,Tussiwand R,Briseño CG,Wu X,Grajales-Reyes GE,Egawa T,Murphy TL,Murphy KM

MHCD0404 was used in Flow cytometry/Cell sorting to study the mechanism by which the abundance of IRF4 induces distinct types of responses.

Mon May 01 00:00:00 EDT 2017

Vpu-Mediated Counteraction of Tetherin Is a Major Determinant of HIV-1 Interferon Resistance.

mBio

Kmiec D,Iyer SS,Stürzel CM,Sauter D,Hahn BH,Kirchhoff F

MHCD0404 was used in flow cytometry elucidate the impact of Vpu-mediated tetherin antagonism on HIV infection

Tue Aug 16 00:00:00 EDT 2016

TLR and NLRP3 inflammasome-dependent innate immune responses to tumor-derived autophagosomes (DRibbles).

Cell death & disease

Xing Y,Cao R,Hu HM

MHCD0404 was used in flow cytometry to elucidate the mechanisms by which DRibbles induce T-cell activation

Thu Aug 04 00:00:00 EDT 2016

Janus kinase inhibition suppresses PKC-induced cytokine release without affecting HIV-1 latency reversal ex vivo.

Retrovirology

Spivak AM,Larragoite ET,Coletti ML,Macedo AB,Martins LJ,Bosque A,Planelles V

MHCD0404 was used in flow cytometry to search for compounds that suppress pro-inflammatory cytokine production in the context of protein kinase C activation

Tue Dec 20 00:00:00 EST 2016

Non-POU Domain-Containing Octamer-Binding Protein Negatively Regulates HIV-1 Infection in CD4(+) T Cells.

AIDS research and human retroviruses

St Gelais C,Roger J,Wu L

MHCD0404 was used in flow cytometry to study the effect of non-POU domain-containing octamer-binding protein (NonO) on the HIV-1 life cycle in CD4(+) T cells

Sat Aug 01 00:00:00 EDT 2015

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

MHCD0404 was used in flow cytometry to investigate how heme regulates Spic in macrophages.

Thu Mar 13 00:00:00 EDT 2014

Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.

PloS one

de Carvalho JV,de Castro RO,da Silva EZ,Silveira PP,da Silva-Januário ME,Arruda E,Jamur MC,Oliver C,Aguiar RS,daSilva LL

MHCD0404 was used in flow cytometry and immunocytochemistry to test if Nef modifies the composition of exosomes released by T lymphocytes

Tue Aug 04 00:00:00 EDT 2015

Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.

PloS one

de Carvalho JV,de Castro RO,da Silva EZ,Silveira PP,da Silva-Januário ME,Arruda E,Jamur MC,Oliver C,Aguiar RS,daSilva LL

MHCD0404 was used in flow cytometry and immunocytochemistry to test if Nef modifies the composition of exosomes released by T lymphocytes

Tue Aug 04 00:00:00 EDT 2015

Anti-inflammatory/regulatory cytokine microenvironment mediated by IL-4 and IL-10 coordinates the immune response in hemophilia A patients infected chronically with hepatitis C virus.

Journal of medical virology

Pimentel JP,Chaves DG,Araújo AR,de Araújo EM,da Silva Fraporti L,Neves WL,Tarragô AM,Torres KL,Gentz SH,Teixeira-Carvalho A,Martins-Filho OA,Malheiro A

Published figure using CD4 monoclonal antibody (Product # MHCD0404) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2013

Immunological/virological peripheral blood biomarkers and distinct patterns of sleeping quality in chronic hepatitis C patients.

Scandinavian journal of immunology

de Almeida CM,de Lima TA,Castro DB,Torres KL,da Silva Braga W,Peruhype-Magalhães V,Teixeira-Carvalho A,Martins-Filho OA,Malheiro A

MHCD0404 was used in flow cytometry to identify immunological/virological biomarkers associated with sleep quality in patients with chronic hepatitis C.

Sun May 01 00:00:00 EDT 2011

Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.

PloS one

Barbosa RR,Silva SP,Silva SL,Melo AC,Pedro E,Barbosa MP,Pereira-Santos MC,Victorino RM,Sousa AE

MHCD0404 was used in flow cytometry to elucidate the link between Th17-cell homeostasis and B cells.

Wed Dec 14 00:00:00 EST 2011

The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.

Journal of cellular and molecular medicine

Elsner L,Flügge PF,Lozano J,Muppala V,Eiz-Vesper B,Demiroglu SY,Malzahn D,Herrmann T,Brunner E,Bickeböller H,Multhoff G,Walter L,Dressel R

MHCD0404 was used in flow cytometry to report that HSP70 activates human NK cells to kill target cells expressing MICA in a natural killer group 2 member D-dependent manner