Invitrogen

CD4 Monoclonal Antibody (S3.5), APC

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

18个已发表图片

35 篇参考文献

查看其他440个CD4抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 21

CD4 Antibody (MHCD0405) in Flow

Detection of human IFN-gamma and TNF-a cytokine-producing human CD4 T cells using the Attune® Acoustic Focusing Cytometer. Red blood cell lysed peripheral blood mononuclear cells were left unstimulated (left column) or stimulated (right column) for 5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. Cells were then fixed and permeabilized using the FIX & PERM® Cell Permeabilization Kit (Product # GAS-003 or Product # GAS-004) and stained intracellularly with anti-human CD4 APC (Product # MHCD0405) and... View More

Product Image Gallery

CD4 Antibody — Figure 4. Effect of PEITC on T cells levels. Peripheral blood mononuclear cells (PBMCs) were collected from blood obtained from control and PEITC treated MDA-MB-231 tumor-bearing SCID/NSG mice (n = 3). Modulation of CD4 was monitored by immunostaining and fluorescence cytometry to determine the effects of PEITC specifically on CD4 + T cells; statistical analysis was performed by Student's t test. ( A ) Representative histograms showing reduced CD4 + lymphocytes ( B ) Bar charts showing mean percentage of lymphocytes positive for CD4 in control and PEITC treated samples. {TM}

产品信息

MHCD0405

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看25篇已发表文章 25篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA, sucrose

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10373698

产品详细信息

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

An ultrasensitive planar array p24 Gag ELISA to detect HIV-1 in diverse biological matrixes.

Scientific reports

Levinger C,Howard JN,Cheng J,Tang P,Joshi A,Catalfamo M,Bosque A

Published figure using CD4 monoclonal antibody (Product # MHCD0405) in Flow Cytometry

Wed Dec 08 00:00:00 EST 2021

Broadly active zinc finger protein-guided transcriptional activation of HIV-1.

Molecular therapy. Methods & clinical development

Scott TA,O'Meally D,Grepo NA,Soemardy C,Lazar DC,Zheng Y,Weinberg MS,Planelles V,Morris KV

MHCD0405 was used in Flow cytometry/Cell sorting to provide proof of concept for the application of a novel, therapeutically relevant, protein transactivator to purge cellular reservoirs of HIV-1.

Fri Mar 12 00:00:00 EST 2021

Broadly active zinc finger protein-guided transcriptional activation of HIV-1.

Molecular therapy. Methods & clinical development

Scott TA,O'Meally D,Grepo NA,Soemardy C,Lazar DC,Zheng Y,Weinberg MS,Planelles V,Morris KV

MHCD0405 was used in Flow cytometry/Cell sorting to provide proof of concept for the application of a novel, therapeutically relevant, protein transactivator to purge cellular reservoirs of HIV-1.

Fri Mar 12 00:00:00 EST 2021

High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture.

Scientific reports

Nesspor TC,Kinealy K,Mazzanti N,Diem MD,Boye K,Hoffman H,Springer C,Sprenkle J,Powers G,Jiang H,La Porte SL,Ganesan R,Singh S,Zwolak A

MHCD0405 was used in Functional assays to describe a high-throughput method for generation of Bipods based on protein A and CH1 domain affinity capture.

Tue May 05 00:00:00 EDT 2020

High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture.

Scientific reports

Nesspor TC,Kinealy K,Mazzanti N,Diem MD,Boye K,Hoffman H,Springer C,Sprenkle J,Powers G,Jiang H,La Porte SL,Ganesan R,Singh S,Zwolak A

MHCD0405 was used in Functional assays to describe a high-throughput method for generation of Bipods based on protein A and CH1 domain affinity capture.

Tue May 05 00:00:00 EDT 2020

MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment.

Nature

Calvanese V,Nguyen AT,Bolan TJ,Vavilina A,Su T,Lee LK,Wang Y,Lay FD,Magnusson M,Crooks GM,Kurdistani SK,Mikkola HKA

MHCD0405 was used in Flow cytometry/Cell sorting to identify MLLT3 (also known as AF9) as a crucial regulator of HSCs that is highly enriched in human fetal, neonatal and adult HSCs, but downregulated in culture.

Sun Dec 01 00:00:00 EST 2019

MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment.

Nature

Calvanese V,Nguyen AT,Bolan TJ,Vavilina A,Su T,Lee LK,Wang Y,Lay FD,Magnusson M,Crooks GM,Kurdistani SK,Mikkola HKA

Sun Dec 01 00:00:00 EST 2019

Structural Basis for Tetherin Antagonism as a Barrier to Zoonotic Lentiviral Transmission.

Cell host & microbe

Buffalo CZ,Stürzel CM,Heusinger E,Kmiec D,Kirchhoff F,Hurley JH,Ren X

MHCD0405 was used in Flow cytometry/Cell sorting to determine the molecular basis for tetherin counteraction and the structure of AP-2 complex with a simian tetherin and simian immunodeficiency virus Nef by cryoelectron microscopy.

Wed Sep 11 00:00:00 EDT 2019

Transcriptional Circuit Fragility Influences HIV Proviral Fate.

Cell reports

Morton EL,Forst CV,Zheng Y,DePaula-Silva AB,Ramirez NP,Planelles V,D'Orso I

MHCD0405 was used in Flow cytometry/Cell sorting to study the effect of transcriptional fragility on HIV viral fate and cure efforts.

Tue Apr 02 00:00:00 EDT 2019

Human-Specific Adaptations in Vpu Conferring Anti-tetherin Activity Are Critical for Efficient Early HIV-1 Replication In Vivo.

Cell host & microbe

Yamada E,Nakaoka S,Klein L,Reith E,Langer S,Hopfensperger K,Iwami S,Schreiber G,Kirchhoff F,Koyanagi Y,Sauter D,Sato K

MHCD0405 was used in Flow cytometry/Cell sorting to demonstrate that the anti-tetherin activity of Vpu is a prerequisite for efficient viral spread during the early phase of infection.

Wed Jan 10 00:00:00 EST 2018

Preadaptation of Simian Immunodeficiency Virus SIVsmm Facilitated Env-Mediated Counteraction of Human Tetherin by Human Immunodeficiency Virus Type 2.

Journal of virology

Heusinger E,Deppe K,Sette P,Krapp C,Kmiec D,Kluge SF,Marx PA,Apetrei C,Kirchhoff F,Sauter D

MHCD0405 was used in Flow cytometry/Cell sorting to investigate whether or how different groups of HIV-2 antagonise human tetherin.

Sat Sep 15 00:00:00 EDT 2018

Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis.

Experimental and therapeutic medicine

Xia Q,Zhou Y,Wang X,Fu S

MHCD0405 was used in Flow cytometry/Cell sorting to evaluate the expression and function of IL-1 receptor-associated kinase 3 in blood mononuclear cells from a model of sepsis-induced immunosuppression.

Thu Feb 01 00:00:00 EST 2018

Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity.

Nature communications

Joas S,Parrish EH,Gnanadurai CW,Lump E,Stürzel CM,Parrish NF,Learn GH,Sauermann U,Neumann B,Rensing KM,Fuchs D,Billingsley JM,Bosinger SE,Silvestri G,Apetrei C,Huot N,Garcia-Tellez T,Müller-Trutwin M,Hotter D,Sauter D,Stahl-Hennig C,Hahn BH,Kirchhoff F

MHCD0405 was used in Flow cytometry/Cell sorting to incorporate two putative HIV-1 virulence determinants, Vpu and Nef, into a non-pathogenic simian immunodeficiency virus (SIV)agm strain and test their impact in African green monkeys.

Tue Apr 10 00:00:00 EDT 2018

Endocytic sorting motif interactions involved in Nef-mediated downmodulation of CD4 and CD3.

Nature communications

Manrique S,Sauter D,Horenkamp FA,Lülf S,Yu H,Hotter D,Anand K,Kirchhoff F,Geyer M

Published figure using CD4 monoclonal antibody (Product # MHCD0405) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

MHCD0405 was used in Flow cytometry/Cell sorting to reveal that TIGIT+CD4+T cells provide a supportive microenvironment for CLL cells.

Sat Jan 29 00:00:00 EST 2022

Potent antitumour activity of interleukin-2-Fc fusion proteins requires Fc-mediated depletion of regulatory T-cells.

Nature communications

Vazquez-Lombardi R,Loetsch C,Zinkl D,Jackson J,Schofield P,Deenick EK,King C,Phan TG,Webster KE,Sprent J,Christ D

MHCD0405 was used in Flow cytometry/Cell sorting to underpin an unexpected mechanism of action and provide important guidance for the development of next generation IL-2 therapeutics.

Fri May 12 00:00:00 EDT 2017

Medial HOXA genes demarcate haematopoietic stem cell fate during human development.

Nature cell biology

Dou DR,Calvanese V,Sierra MI,Nguyen AT,Minasian A,Saarikoski P,Sasidharan R,Ramirez CM,Zack JA,Crooks GM,Galic Z,Mikkola HK

MHCD0405 was used in flow cytometry to study how retinoic acid-induced HOXA gene expression controls hematopoietic stem/progenitor cells fate, identify, and function

Wed Jun 01 00:00:00 EDT 2016

Modeling HIV-1 Latency in Primary T Cells Using a Replication-Competent Virus.

AIDS research and human retroviruses

Martins LJ,Bonczkowski P,Spivak AM,De Spiegelaere W,Novis CL,DePaula-Silva AB,Malatinkova E,Trypsteen W,Bosque A,Vanderkerckhove L,Planelles V

MHCD0405 was used in flow cytometry to develop a model that uses cultured central memory CD4 positive T cells and replication-competent HIV-1

Mon Feb 01 00:00:00 EST 2016

Vpu-Mediated Counteraction of Tetherin Is a Major Determinant of HIV-1 Interferon Resistance.

mBio

Kmiec D,Iyer SS,Stürzel CM,Sauter D,Hahn BH,Kirchhoff F

MHCD0405 was used in flow cytometry elucidate the impact of Vpu-mediated tetherin antagonism on HIV infection

Tue Aug 16 00:00:00 EDT 2016

A Naturally Occurring rev1-vpu Fusion Gene Does Not Confer a Fitness Advantage to HIV-1.

PloS one

Langer SM,Hopfensperger K,Iyer SS,Kreider EF,Learn GH,Lee LH,Hahn BH,Sauter D

Published figure using CD4 monoclonal antibody (Product # MHCD0405) in Flow Cytometry

Tue Jun 14 00:00:00 EDT 2016

PEITC treatment suppresses myeloid derived tumor suppressor cells to inhibit breast tumor growth.

Oncoimmunology

Gupta P,Wright SE,Srivastava SK

Published figure using CD4 monoclonal antibody (Product # MHCD0405) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells.

eLife

Reeder JE,Kwak YT,McNamara RP,Forst CV,D'Orso I

Published figure using CD4 monoclonal antibody (Product # MHCD0405) in Flow Cytometry

Wed Oct 21 00:00:00 EDT 2015

An HIV-encoded antisense long noncoding RNA epigenetically regulates viral transcription.

Molecular therapy : the journal of the American Society of Gene Therapy

Saayman S,Ackley A,Turner AW,Famiglietti M,Bosque A,Clemson M,Planelles V,Morris KV

MHCD0405 was used in flow cytometry to characterize the effects of human immunodeficiency virus lncRNA expression

Sun Jun 01 00:00:00 EDT 2014

Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.

PloS one

de Carvalho JV,de Castro RO,da Silva EZ,Silveira PP,da Silva-Januário ME,Arruda E,Jamur MC,Oliver C,Aguiar RS,daSilva LL

MHCD0405 was used in flow cytometry and immunocytochemistry to test if Nef modifies the composition of exosomes released by T lymphocytes

Tue Aug 04 00:00:00 EDT 2015

Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.

Journal of immunology (Baltimore, Md. : 1950)

Palin AC,Ramachandran V,Acharya S,Lewis DB

MHCD0405 was used in flow cytometry to test if impaired human neonatal CD4(+) T cell immunity is due to reduced signaling by naive CD4(+) T cells following engagement of the αβ-TCR/CD3 complex and CD28.

Fri Mar 15 00:00:00 EDT 2013

The transmembrane domain of HIV-1 Vpu is sufficient to confer anti-tetherin activity to SIVcpz and SIVgor Vpu proteins: cytoplasmic determinants of Vpu function.

Retrovirology

Kluge SF,Sauter D,Vogl M,Peeters M,Li Y,Bibollet-Ruche F,Hahn BH,Kirchhoff F

Published figure using CD4 monoclonal antibody (Product # MHCD0405) in Flow Cytometry

Wed Mar 20 00:00:00 EDT 2013

Subcutaneous versus intravenous administration of rituximab: pharmacokinetics, CD20 target coverage and B-cell depletion in cynomolgus monkeys.

PloS one

Mao CP,Brovarney MR,Dabbagh K,Birnböck HF,Richter WF,Del Nagro CJ

MHCD0405 was used in flow cytometry to assess subcutaneous administration of rituximab.

Fri Jul 25 00:00:00 EDT 2014

Human tetherin exerts strong selection pressure on the HIV-1 group N Vpu protein.

PLoS pathogens

Sauter D,Unterweger D,Vogl M,Usmani SM,Heigele A,Kluge SF,Hermkes E,Moll M,Barker E,Peeters M,Learn GH,Bibollet-Ruche F,Fritz JV,Fackler OT,Hahn BH,Kirchhoff F

Published figure using CD4 monoclonal antibody (Product # MHCD0405) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Helicobacter pylori induces in-vivo expansion of human regulatory T cells through stimulating interleukin-1β production by dendritic cells.

Clinical and experimental immunology

Mitchell PJ,Afzali B,Fazekasova H,Chen D,Ali N,Powell N,Lord GM,Lechler RI,Lombardi G

MHCD0405 was used in flow cytometry to elucidate the influence of H. pylori on T(reg) function and proliferation.

Sat Dec 01 00:00:00 EST 2012

Characterization of an HIV-targeted transcriptional gene-silencing RNA in primary cells.

Human gene therapy

Turner AM,Ackley AM,Matrone MA,Morris KV

MHCD0405 was used in flow cytometry to demonstrate that transcriptional gene silencing reduces HIV transcription in primary human CD4(+) T cells.

Tue May 01 00:00:00 EDT 2012

Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response.

Blood

Gardam S,Turner VM,Anderton H,Limaye S,Basten A,Koentgen F,Vaux DL,Silke J,Brink R

MHCD0405 was used in flow cytometry to determine the role of cIAP1 and cIAP2 to B-cell physiology in vivo.

Thu Apr 14 00:00:00 EDT 2011

A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease

Grahmann PR,Braun RK

MHCD0405 was used in flow cytometry to demonstrate that aerosolized interferon-gamma results in the transient conversion of sputum smears in multidrug-resistant pulmonary tuberculosis