Invitrogen

CD4 Monoclonal Antibody (S3.5), TRI-COLOR™

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CD4 Antibody (MHCD0406) in Flow

Human peripheral blood lymphocytes were stained using TRI-COLOR® of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.

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产品信息

MHCD0406

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看4篇已发表文章 4篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

TRI-COLOR™ TRI-COLOR™ TRI-COLOR™

激发/发射光谱

567/671 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA, sucrose

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10392548

产品详细信息

The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5® conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

MHCD0406 was used in Flow cytometry/Cell sorting to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

Intermediate DNA methylation is a conserved signature of genome regulation.

Nature communications

Elliott G,Hong C,Xing X,Zhou X,Li D,Coarfa C,Bell RJ,Maire CL,Ligon KL,Sigaroudinia M,Gascard P,Tlsty TD,Harris RA,Schalkwyk LC,Bilenky M,Mill J,Farnham PJ,Kellis M,Marra MA,Milosavljevic A,Hirst M,Stormo GD,Wang T,Costello JF

MHCD0406 was used in flow cytometry to show that intermediate DNA methylation is a conserved signature of gene regulation and exon usage

Wed Feb 18 00:00:00 EST 2015

Changes in the proportions of CD4(+)T cell subsets defined by CD127 and CD25 expression during HBV infection.

Immunological investigations

Xu HT,Ye J,Chen YB,Zhang LX,Huang JX,Xian JC,Liu L,Peng HL,Li L,Lin M,Huang JH

MHCD0406 was used in flow cytometry to study changes in T cell subsets during HBV infection.

Tue Jul 10 00:00:00 EDT 2012

Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody.

The Journal of infectious diseases

Luiza-Silva M,Campi-Azevedo AC,Batista MA,Martins MA,Avelar RS,da Silveira Lemos D,Bastos Camacho LA,de Menezes Martins R,de Lourdes de Sousa Maia M,Guedes Farias RH,da Silva Freire M,Galler R,Homma A,Leite Ribeiro JG,Campos Lemos JA,Auxiliadora-Martins M,Eloi-Santos SM,Teixeira-Carvalho A,Martins-Filho OA