Invitrogen

CD4 Monoclonal Antibody (S3.5), PE-Cyanine7

5个已发表图片

6 篇参考文献

查看其他440个CD4抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
已发表图片 (5)

图: 1 / 6

CD4 Antibody (MHCD0412) in Flow

Human peripheral blood lymphocytes were stained using PE-Cy®7 of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.

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产品信息

MHCD0412

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看5篇已发表文章 5篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 10% sucrose

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10373809

产品详细信息

The R-phycoerythrin (PE)-Cy7® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a 488 nm laser without occupying traditional channels associated with FITC and PE.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Microbiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis.

Nature communications

Sun M,Wu W,Chen L,Yang W,Huang X,Ma C,Chen F,Xiao Y,Zhao Y,Ma C,Yao S,Carpio VH,Dann SM,Zhao Q,Liu Z,Cong Y

MHCD0412 was used in Flow Cytometry to elucidate the various roles of gut microbiota-derived short-chain fatty acids in the maintenance of intestinal homeostasis.

Mon Sep 03 00:00:00 EDT 2018

Increased ratio of Th17 cells to SIGIRR⁺CD4⁺ T cells in peripheral blood of patients with SLE is associated with disease activity.

Biomedical reports

Xiao JP,Wang DY,Wang XR,Yuan L,Hao L,Wang DG

MHCD0412 was used in Flow cytometry/Cell sorting to investigate the ratio of T helper cell 17 cells to single immunoglobulin IL-1-related receptor cluster of differentiation T cells in patients with systemic lupus erythematosus.

Mon Oct 01 00:00:00 EDT 2018

A novel method for assaying human regulatory T cell direct suppression of B cell effector function.

Journal of immunological methods

Weingartner E,Courneya JP,Keegan A,Golding A

MHCD0412 was used in flow cytometry to develop a method to assess the capacity of human regulatory T cells to suppress IgM production from an immature human B cell line

Wed Feb 01 00:00:00 EST 2017

A novel method for assaying human regulatory T cell direct suppression of B cell effector function.

Journal of immunological methods

Weingartner E,Courneya JP,Keegan A,Golding A

MHCD0412 was used in flow cytometry to develop a method to assess the capacity of human regulatory T cells to suppress IgM production from an immature human B cell line

Wed Feb 01 00:00:00 EST 2017

Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1.

PLoS biology

Amarnath S,Costanzo CM,Mariotti J,Ullman JL,Telford WG,Kapoor V,Riley JL,Levine BL,June CH,Fong T,Warner NL,Fowler DH

MHCD0412 was used in flow cytometry to report that human Tregs promote the generation of myeloid dendritic cells which abrogate post transplant lethality.

Tue Feb 02 00:00:00 EST 2010

Apoptosis of CD4(+)CD25(high) T cells in response to Sirolimus requires activation of T cell receptor and is modulated by IL-2.

Cancer immunology, immunotherapy : CII

Molhoek KR,McSkimming CC,Olson WC,Brautigan DL,Slingluff CL

Mon Jun 01 00:00:00 EDT 2009

A phase I/II study of a MUC1 peptide pulsed autologous dendritic cell vaccine as adjuvant therapy in patients with resected pancreatic and biliary tumors.

Cancer therapy

Lepisto AJ,Moser AJ,Zeh H,Lee K,Bartlett D,McKolanis JR,Geller BA,Schmotzer A,Potter DP,Whiteside T,Finn OJ,Ramanathan RK

MHCD0412 was used in Flow cytometry/Cell sorting to study the use of a MUC1 peptide pulsed autologous dendritic cell vaccine as adjuvant therapy in patients with resected pancreatic and biliary tumours.