Invitrogen

CD4 Monoclonal Antibody (S3.5), PE-Texas Red

22个已发表图片

26 篇参考文献

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实验方案

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说明书

实验方案

技术支持

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CD4 Antibody (MHCD0417) in Flow

Human peripheral blood lymphocytes were stained using PE-Texas Red® of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.

CD4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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产品信息

MHCD0417

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看18篇已发表文章 18篇已发表文章

其他PubMed (Misc)

查看6篇已发表文章 6篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

PE-Texas Red PE-Texas Red PE-Texas Red

激发/发射光谱

567/615 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 10% sucrose, BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10371766

产品详细信息

The R-phycoerythrin (PE)-Texas Red conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Analyte Specific Reagent

How to use the Panel Builder

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Product References

Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.

iScience

McDavid A,Laniewski N,Grier A,Gill AL,Kessler HA,Huyck H,Carbonell E,Holden-Wiltse J,Bandyopadhyay S,Carnahan J,Dylag AM,Topham DJ,Falsey AR,Caserta MT,Pryhuber GS,Gill SR,Scheible KM

MHCD0417 was used in Flow Cytometry to conclude that early exposures predict the postnatal immune-microbiota axis trajectory, placing infants at later risk for respiratory morbidity in early childhood.

Fri Apr 15 00:00:00 EDT 2022

Broadly reactive human CD4⁺ T cells against Enterobacteriaceae are found in the naïve repertoire and are clonally expanded in the memory repertoire.

European journal of immunology

Cassotta A,Goldstein JD,Durini G,Jarrossay D,Baggi Menozzi F,Venditti M,Russo A,Falcone M,Lanzavecchia A,Gagliardi MC,Latorre D,Sallusto F

MHCD0417 was used in Flow cytometry/Cell sorting to demonstrate that a large fraction of memory Th cell clones was broadly cross-reactive to several Enterobacteriaceae species.

Mon Mar 01 00:00:00 EST 2021

Broadly reactive human CD4⁺ T cells against Enterobacteriaceae are found in the naïve repertoire and are clonally expanded in the memory repertoire.

European journal of immunology

Cassotta A,Goldstein JD,Durini G,Jarrossay D,Baggi Menozzi F,Venditti M,Russo A,Falcone M,Lanzavecchia A,Gagliardi MC,Latorre D,Sallusto F

MHCD0417 was used in Flow cytometry/Cell sorting to demonstrate that a large fraction of memory Th cell clones was broadly cross-reactive to several Enterobacteriaceae species.

Mon Mar 01 00:00:00 EST 2021

Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin.

The Journal of experimental medicine

Cassotta A,Paparoditis P,Geiger R,Mettu RR,Landry SJ,Donati A,Benevento M,Foglierini M,Lewis DJM,Lanzavecchia A,Sallusto F

MHCD0417 was used in Flow cytometry/Cell sorting to show the presence of a broad repertoire of naive T cells specific for cryptic H1-HA peptides and demonstrate that antigen processing represents a major constrain determining immunodominance.

Mon Oct 05 00:00:00 EDT 2020

Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin.

The Journal of experimental medicine

Cassotta A,Paparoditis P,Geiger R,Mettu RR,Landry SJ,Donati A,Benevento M,Foglierini M,Lewis DJM,Lanzavecchia A,Sallusto F

Mon Oct 05 00:00:00 EDT 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

MHCD0417 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

MHCD0417 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

CD4-Dependent Modulation of HIV-1 Entry by LY6E.

Journal of virology

Yu J,Liang C,Liu SL

MHCD0417 was used in Flow cytometry/Cell sorting to show lymphocyte antigen 6E inhibits HIV-1 entry and replication in low-CD4-expressing MDMs and Jurkat cells, while facilitating viral membrane fusion in high-CD4 expressing cells.

Mon Apr 01 00:00:00 EDT 2019

CD4-Dependent Modulation of HIV-1 Entry by LY6E.

Journal of virology

Yu J,Liang C,Liu SL

Mon Apr 01 00:00:00 EDT 2019

Lower Interferon Regulatory Factor-8 Expression in Peripheral Myeloid Cells Tracks With Adverse Central Nervous System Outcomes in Treated HIV Infection.

Frontiers in immunology

D'Antoni ML,Kallianpur KJ,Premeaux TA,Corley MJ,Fujita T,Laws EI,Ogata-Arakaki D,Chow DC,Khadka VS,Shikuma CM,Ndhlovu LC

Published figure using CD4 monoclonal antibody (Product # MHCD0417) in Flow Cytometry

Tue Nov 03 00:00:00 EST 2020

RNA-seq and flow-cytometry of conventional, scalp, and palmoplantar psoriasis reveal shared and distinct molecular pathways.

Scientific reports

Ahn R,Yan D,Chang HW,Lee K,Bhattarai S,Huang ZM,Nakamura M,Singh R,Afifi L,Taravati K,Munoz-Sandoval P,Pauli M,Rosenblum MD,Liao W

MHCD0417 was used in Flow cytometry/Cell sorting to show the molecular heterogeneity of plaque psoriasis and identify the subtype specific signalling pathways.

Fri Jul 27 00:00:00 EDT 2018

High frequency of intestinal T_H17 cells correlates with microbiota alterations and disease activity in multiple sclerosis.

Science advances

Cosorich I,Dalla-Costa G,Sorini C,Ferrarese R,Messina MJ,Dolpady J,Radice E,Mariani A,Testoni PA,Canducci F,Comi G,Martinelli V,Falcone M

MHCD0417 was used in Flow cytometry/Cell sorting to assess the crucial role of the intestinal environment in promoting Th17 cell expansion in human multiple sclerosis patients.

Sat Jul 01 00:00:00 EDT 2017

DNA cytosine hydroxymethylation levels are distinct among non-overlapping classes of peripheral blood leukocytes.

Journal of immunological methods

Hohos NM,Lee K,Ji L,Yu M,Kandasamy MM,Phillips BG,Baile CA,He C,Schmitz RJ,Meagher RB

MHCD0417 was used in Immunocytochemistry-immunoflourescence to suggest a hierarchy of responsiveness among classes of leukocytes with varying methylome response to physiological stress and disease.

Thu Sep 01 00:00:00 EDT 2016

Tumor-infiltrating Tim-3⁺ T cells proliferate avidly except when PD-1 is co-expressed: Evidence for intracellular cross talk.

Oncoimmunology

Li J,Shayan G,Avery L,Jie HB,Gildener-Leapman N,Schmitt N,Lu BF,Kane LP,Ferris RL

Published figure using CD4 monoclonal antibody (Product # MHCD0417) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Plasmacytoid dendritic cells promote HIV-1-induced group 3 innate lymphoid cell depletion.

The Journal of clinical investigation

Zhang Z,Cheng L,Zhao J,Li G,Zhang L,Chen W,Nie W,Reszka-Blanco NJ,Wang FS,Su L

Published figure using CD4 monoclonal antibody (Product # MHCD0417) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Deng N,Mosmann TR

MHCD0417 was used in flow cytometry to optimize detection of IL-2 from live cells

Sat Aug 01 00:00:00 EDT 2015

Select host restriction factors are associated with HIV persistence during antiretroviral therapy.

AIDS (London, England)

Abdel-Mohsen M,Wang C,Strain MC,Lada SM,Deng X,Cockerham LR,Pilcher CD,Hecht FM,Liegler T,Richman DD,Deeks SG,Pillai SK

MHCD0417 was used in flow cytometry to ascertain the impact of cell-intrinsic immunity on the latent reservoir of HIV

Fri Feb 20 00:00:00 EST 2015

Isolation and cytokine analysis of lamina propria lymphocytes from mucosal biopsies of the human colon.

Journal of immunological methods

Bowcutt R,Malter LB,Chen LA,Wolff MJ,Robertson I,Rifkin DB,Poles M,Cho I,Loke P

MHCD0417 was used in flow cytometry to analyze mucosal pinch biopsies collected predominantly during colonoscopies.

Mon Jun 01 00:00:00 EDT 2015

High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.

Cancer research

Péguillet I,Milder M,Louis D,Vincent-Salomon A,Dorval T,Piperno-Neumann S,Scholl SM,Lantz O

MHCD0417 was used in flow cytometry to examine human differentiated effector CD4(+) T cells that are defined by low levels of IL-2 and IL-7 receptors.

Tue Apr 15 00:00:00 EDT 2014

Plasmacytoid dendritic cells suppress HIV-1 replication but contribute to HIV-1 induced immunopathogenesis in humanized mice.

PLoS pathogens

Li G,Cheng M,Nunoya J,Cheng L,Guo H,Yu H,Liu YJ,Su L,Zhang L

MHCD0417 was used in flow cytometry to study the role of plasmacytoid dendritic cells in human immunodeficiency virus type 1 infection and pathogenesis

Tue Jul 01 00:00:00 EDT 2014

Highly heterogeneous, activated, and short-lived regulatory T cells during chronic filarial infection.

European journal of immunology

Metenou S,Coulibaly YI,Sturdevant D,Dolo H,Diallo AA,Soumaoro L,Coulibaly ME,Kanakabandi K,Porcella SF,Klion AD,Nutman TB

MHCD0417 was used in Flow cytometry/Cell sorting to study how in filarial infection, the expanded Treg-cell populations are heterogeneous, short-lived, activated, and express higher levels of molecules known to modulate immune responsiveness.

Tue Jul 01 00:00:00 EDT 2014

Concomitant evaluation of PMA+ionomycin-induced kinase phosphorylation and cytokine production in T cell subsets by flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Crawford TQ,Jalbert E,Ndhlovu LC,Barbour JD

Published figure using CD4 monoclonal antibody (Product # MHCD0417) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

B cell responses to HIV antigen are a potent correlate of viremia in HIV-1 infection and improve with PD-1 blockade.

PloS one

Nicholas KJ,Zern EK,Barnett L,Smith RM,Lorey SL,Copeland CA,Sadagopal S,Kalams SA

MHCD0417 was used in flow cytometry to assess the extent of B cell dysfunction during HIV infection.

Tue Sep 16 00:00:00 EDT 2014

Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.

Vaccine

Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR

MHCD0417 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.

Fri Jun 29 00:00:00 EDT 2012

TH17, TH22 and Treg cells are enriched in the healthy human cecum.

PloS one

Wolff MJ,Leung JM,Davenport M,Poles MA,Cho I,Loke P

Published figure using CD4 monoclonal antibody (Product # MHCD0417) in Flow Cytometry

Fri Dec 07 00:00:00 EST 2012

Uncoupling of proliferation and cytokines from suppression within the CD4+CD25+Foxp3+ T-cell compartment in the 1st year of human type 1 diabetes.

Diabetes

Hughson A,Bromberg I,Johnson B,Quataert S,Jospe N,Fowell DJ

MHCD0417 was used in Flow cytometry/Cell sorting to determine the relative proportions of effector and regulatory T cells in the CD4+CD25+Foxp3+ T-cell compartment in varying type 1 diabetes disease states.

Mon Aug 01 00:00:00 EDT 2011

HIV-1 infection abrogates CD8+ T cell mitogen-activated protein kinase signaling responses.

Journal of virology

Crawford TQ,Ndhlovu LC,Tan A,Carvidi A,Hecht FM,Sinclair E,Barbour JD

MHCD0417 was used in flow cytometry to elucidate relationships among MAPK pathway function, HIV-1-induced activation, and exhaustion markers in circulating CD8+ T cells