Invitrogen

CD4 Monoclonal Antibody (S3.5), Pacific Blue™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6个已发表图片

6 篇参考文献

查看其他441个CD4抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 9

CD4 Antibody (MHCD0428) in Flow

Human peripheral blood lymphocytes were stained using Pacific Blue™ of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.

Product Image Gallery

CD4 Antibody — Fig. 3 Tumor infiltrating lymphocytes (TIL) in the anti-GITR (1)/SRS group have a Th1 immunophenotype and elevated effector to Treg ratio. C57BL/6 mice were inoculated with GL261-luc tumor, randomized to groups of >=5, and dosed with anti-GITR (1) and SRS as in Fig. 1 . Mice were sacrificed on day 21, tumor infiltrating lymphocytes were isolated, cells were stimulated with PMA/Ionomycin, fixed, and permeabilized for staining of intracellular markers. a CD8+ and b CD4+ cell populations were analyzed by flow cytometry for expression of IFNgamma, TNFalpha, and IL-2. Intratumoral effector CD8+ and CD4+ to Treg ratios in the anti-GITR (1)/SRS group were calculated c . Symbol and horizontal bar denote single mouse and average value, respectively. * P < .05 {TM}

产品信息

MHCD0428

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL/1x10^6 cells

查看5篇已发表文章 5篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S3.5

抗原

Human CD4

偶联物

Pacific Blue™ Pacific Blue™ Pacific Blue™

激发/发射光谱

404/455 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10371780

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

A Biological Circuit Involving Mef2c, Mef2d, and Hdac9 Controls the Immunosuppressive Functions of CD4+Foxp3+ T-Regulatory Cells.

Frontiers in immunology

Di Giorgio E,Wang L,Xiong Y,Christensen LM,Akimova T,Han R,Samanta A,Trevisanut M,Brancolini C,Beier UH,Hancock WW

MHCD0428 was used in Flow Cytometry to report that as with Mef2d, the deletion of Mef2c in Tregs switches off the expression of Il10 and Icos and leads to enhanced antitumor immunity in syngeneic models of lung cancer.

Fri Dec 17 00:00:00 EST 2021

A Biological Circuit Involving Mef2c, Mef2d, and Hdac9 Controls the Immunosuppressive Functions of CD4+Foxp3+ T-Regulatory Cells.

Frontiers in immunology

Di Giorgio E,Wang L,Xiong Y,Christensen LM,Akimova T,Han R,Samanta A,Trevisanut M,Brancolini C,Beier UH,Hancock WW

Fri Dec 17 00:00:00 EST 2021

Inhibiting the coregulator CoREST impairs Foxp3+ Treg function and promotes antitumor immunity.

The Journal of clinical investigation

Xiong Y,Wang L,Di Giorgio E,Akimova T,Beier UH,Han R,Trevisanut M,Kalin JH,Cole PA,Hancock WW

MHCD0428 was used in Flow cytometry/Cell sorting to analyse the role in Tregs of the evolutionarily conserved CoREST complex, consisting of a scaffolding protein, Rcor1 or Rcor2, plus Hdac1 or Hdac2 and Lsd1 enzymes.

Wed Apr 01 00:00:00 EDT 2020

Inhibiting the coregulator CoREST impairs Foxp3+ Treg function and promotes antitumor immunity.

The Journal of clinical investigation

Xiong Y,Wang L,Di Giorgio E,Akimova T,Beier UH,Han R,Trevisanut M,Kalin JH,Cole PA,Hancock WW

Wed Apr 01 00:00:00 EDT 2020

MEF2D sustains activation of effector Foxp3+ Tregs during transplant survival and anticancer immunity.

The Journal of clinical investigation

Di Giorgio E,Wang L,Xiong Y,Akimova T,Christensen LM,Han R,Samanta A,Trevisanut M,Bhatti TR,Beier UH,Hancock WW

MHCD0428 was used in Flow cytometry/Cell sorting to study the role played by MEF2D in sustaining effector Foxp3+ Treg functions without abrogating their basal actions suggests its suitability for drug discovery efforts in cancer therapy.

Tue Dec 01 00:00:00 EST 2020

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

MHCD0428 was used in flow cytometry to analyze the induction of immune-mediated survival advantage in murine intracranial glioma by agonist anti-GITR monoclonal antibody and stereotactic radation

Wed May 18 00:00:00 EDT 2016

T-cell receptor repertoire of human peripheral CD161hiTRAV1-2+ MAIT cells revealed by next generation sequencing and single cell analysis.

Human immunology

Held K,Beltrán E,Moser M,Hohlfeld R,Dornmair K

MHCD0428TR was used in flow cytometry to describe the CD6(hi)TRAV-2(+) TCR repertoire.

Tue Sep 01 00:00:00 EDT 2015

High content cellular immune profiling reveals differences between rhesus monkeys and men.

Immunology

Magalhaes I,Vudattu NK,Ahmed RK,Kühlmann-Berenzon S,Ngo Y,Sizemore DR,Wehlin L,Weichold F,Andersson J,Skeiky YA,Sadoff J,Gaines H,Thorstensson R,Spångberg M,Maeurer MJ