Western Blot全流程视频

Western Blot 是一个多步骤的实验流程,根据所用方法的不同,可能需要数小时或 1-2天才能完成。以下演示视频,覆盖Western Blot各个环节,详细介绍了从电泳分离蛋白,转膜、印迹孵育、检测、成像到剥离这6大步骤的实验原理、操作方法和注意事项。

根据分子量大小分离蛋白质。

Video Player is loading.
Current Time 0:00
Duration 5:23
Loaded: 3.09%
Stream Type LIVE
Remaining Time 5:23
 
1x
    • Chapters
    • descriptions off, selected
    • subtitles off, selected
    • en (Main), selected

    将蛋白质从凝胶转印到印迹膜上。

    Video Player is loading.
    Current Time 0:00
    Duration 4:05
    Loaded: 4.08%
    Stream Type LIVE
    Remaining Time 4:05
     
    1x
      • Chapters
      • descriptions off, selected
      • subtitles off, selected
      • en (Main), selected

      如何使用 Invitrogen Mini Blot 模块进行湿转

      Video Player is loading.
      Current Time 0:00
      Duration 3:53
      Loaded: 0.00%
      Stream Type LIVE
      Remaining Time 3:53
       
      1x
        • Chapters
        • descriptions off, selected
        • subtitles off, selected
        • en (Main), selected

        如何使用 Invitrogen Power Blotter 和即用型转印膜组进行快速半干转

        Video Player is loading.
        Current Time 0:00
        Duration 4:27
        Loaded: 3.74%
        Stream Type LIVE
        Remaining Time 4:27
         
        1x
          • Chapters
          • descriptions off, selected
          • subtitles off, selected
          • en (Main), selected

          如何使用Invitrogen Power Blotter和预切膜与滤纸进行快速半干转

          Video Player is loading.
          Current Time 0:00
          Duration 3:29
          Loaded: 4.77%
          Stream Type LIVE
          Remaining Time 3:29
           
          1x
            • Chapters
            • descriptions off, selected
            • subtitles off, selected
            • en (Main), selected

            如何使用 Invitrogen iBlot 2转印系统7分钟快速干转

            封闭膜上未结合的位点,以降低非特异性信号。 通过一抗和二抗标记印迹

            Video Player is loading.
            Current Time 0:00
            Duration 3:46
            Loaded: 4.41%
            Stream Type LIVE
            Remaining Time 3:46
             
            1x
              • Chapters
              • descriptions off, selected
              • subtitles off, selected
              • en (Main), selected

              如何进行印迹膜的封闭和抗体孵育

              Video Player is loading.
              Current Time 0:00
              Duration 4:25
              Loaded: 0.00%
              Stream Type LIVE
              Remaining Time 4:25
               
              1x
                • Chapters
                • descriptions off, selected
                • subtitles off, selected
                • en (Main), selected

                如何使用 Invitrogen iBind系统自动化处理印迹膜

                根据你选择的实验体系检测蛋白质。

                Video Player is loading.
                Current Time 0:00
                Duration 3:05
                Loaded: 0.00%
                Stream Type LIVE
                Remaining Time 3:05
                 
                1x
                  • Chapters
                  • descriptions off, selected
                  • subtitles off, selected
                  • en (Main), selected

                  如何使用化学发光检测试剂检测目标蛋白

                  Video Player is loading.
                  Current Time 0:00
                  Duration 5:37
                  Loaded: 0.00%
                  Stream Type LIVE
                  Remaining Time 5:37
                   
                  1x
                    • Chapters
                    • descriptions off, selected
                    • subtitles off, selected
                    • en (Main), selected

                    捕捉并分析你的图像。

                    Video Player is loading.
                    Current Time 0:00
                    Duration 3:19
                    Loaded: 4.97%
                    Stream Type LIVE
                    Remaining Time 3:19
                     
                    1x
                      • Chapters
                      • descriptions off, selected
                      • subtitles off, selected
                      • en (Main), selected

                      如何使用 Invitrogen iBright 成像系统拍摄图片

                      Video Player is loading.
                      Current Time 0:00
                      Duration 3:17
                      Loaded: 0.00%
                      Stream Type LIVE
                      Remaining Time 3:17
                       
                      1x
                        • Chapters
                        • descriptions off, selected
                        • subtitles off, selected
                        • en (Main), selected

                        如何使用 Invitrogen iBright 成像系统进行数据分析

                        Video Player is loading.
                        Current Time 0:00
                        Duration 2:13
                        Loaded: 0.00%
                        Stream Type LIVE
                        Remaining Time 2:13
                         
                        1x
                          • Chapters
                          • descriptions off, selected
                          • subtitles off, selected
                          • en (Main), selected

                          如何使用 Invitrogen iBright 成像系统导出图片

                          剥离并在印迹膜上重新检测另一个蛋白靶点

                          Video Player is loading.
                          Current Time 0:00
                          Duration 1:54
                          Loaded: 0.00%
                          Stream Type LIVE
                          Remaining Time 1:54
                           
                          1x
                            • Chapters
                            • descriptions off, selected
                            • subtitles off, selected
                            • en (Main), selected

                            如何剥离抗体并重新检测wb印迹膜

                            根据分子量大小分离蛋白质。

                            Video Player is loading.
                            Current Time 0:00
                            Duration 5:23
                            Loaded: 3.09%
                            Stream Type LIVE
                            Remaining Time 5:23
                             
                            1x
                              • Chapters
                              • descriptions off, selected
                              • subtitles off, selected
                              • en (Main), selected

                              将蛋白质从凝胶转印到印迹膜上。

                              Video Player is loading.
                              Current Time 0:00
                              Duration 4:05
                              Loaded: 4.08%
                              Stream Type LIVE
                              Remaining Time 4:05
                               
                              1x
                                • Chapters
                                • descriptions off, selected
                                • subtitles off, selected
                                • en (Main), selected

                                如何使用 Invitrogen Mini Blot 模块进行湿转

                                Video Player is loading.
                                Current Time 0:00
                                Duration 3:53
                                Loaded: 0.00%
                                Stream Type LIVE
                                Remaining Time 3:53
                                 
                                1x
                                  • Chapters
                                  • descriptions off, selected
                                  • subtitles off, selected
                                  • en (Main), selected

                                  如何使用 Invitrogen Power Blotter 和即用型转印膜组进行快速半干转

                                  Video Player is loading.
                                  Current Time 0:00
                                  Duration 4:27
                                  Loaded: 0.00%
                                  Stream Type LIVE
                                  Remaining Time 4:27
                                   
                                  1x
                                    • Chapters
                                    • descriptions off, selected
                                    • subtitles off, selected
                                    • en (Main), selected

                                    如何使用Invitrogen Power Blotter和预切膜与滤纸进行快速半干转

                                    Video Player is loading.
                                    Current Time 0:00
                                    Duration 3:29
                                    Loaded: 0.00%
                                    Stream Type LIVE
                                    Remaining Time 3:29
                                     
                                    1x
                                      • Chapters
                                      • descriptions off, selected
                                      • subtitles off, selected
                                      • en (Main), selected

                                      如何使用 Invitrogen iBlot 2转印系统7分钟快速干转

                                      封闭膜上未结合的位点,以降低非特异性信号。 通过一抗和二抗标记印迹

                                      Video Player is loading.
                                      Current Time 0:00
                                      Duration 3:46
                                      Loaded: 0.00%
                                      Stream Type LIVE
                                      Remaining Time 3:46
                                       
                                      1x
                                        • Chapters
                                        • descriptions off, selected
                                        • subtitles off, selected
                                        • en (Main), selected

                                        如何进行印迹膜的封闭和抗体孵育

                                        Video Player is loading.
                                        Current Time 0:00
                                        Duration 4:25
                                        Loaded: 0.00%
                                        Stream Type LIVE
                                        Remaining Time 4:25
                                         
                                        1x
                                          • Chapters
                                          • descriptions off, selected
                                          • subtitles off, selected
                                          • en (Main), selected

                                          如何使用 Invitrogen iBind系统自动化处理印迹膜

                                          根据你选择的实验体系检测蛋白质。

                                          Video Player is loading.
                                          Current Time 0:00
                                          Duration 3:05
                                          Loaded: 0.00%
                                          Stream Type LIVE
                                          Remaining Time 3:05
                                           
                                          1x
                                            • Chapters
                                            • descriptions off, selected
                                            • subtitles off, selected
                                            • en (Main), selected

                                            如何使用化学发光检测试剂检测目标蛋白

                                            Video Player is loading.
                                            Current Time 0:00
                                            Duration 5:37
                                            Loaded: 0.00%
                                            Stream Type LIVE
                                            Remaining Time 5:37
                                             
                                            1x
                                              • Chapters
                                              • descriptions off, selected
                                              • subtitles off, selected
                                              • en (Main), selected

                                              捕捉并分析你的图像。

                                              Video Player is loading.
                                              Current Time 0:00
                                              Duration 3:19
                                              Loaded: 0%
                                              Stream Type LIVE
                                              Remaining Time 3:19
                                               
                                              1x
                                                • Chapters
                                                • descriptions off, selected
                                                • subtitles off, selected
                                                • en (Main), selected

                                                如何使用 Invitrogen iBright 成像系统拍摄图片

                                                Video Player is loading.
                                                Current Time 0:00
                                                Duration 3:17
                                                Loaded: 0%
                                                Stream Type LIVE
                                                Remaining Time 3:17
                                                 
                                                1x
                                                  • Chapters
                                                  • descriptions off, selected
                                                  • subtitles off, selected
                                                  • en (Main), selected

                                                  如何使用 Invitrogen iBright 成像系统进行数据分析

                                                  Video Player is loading.
                                                  Current Time 0:00
                                                  Duration 2:13
                                                  Loaded: 0%
                                                  Stream Type LIVE
                                                  Remaining Time 2:13
                                                   
                                                  1x
                                                    • Chapters
                                                    • descriptions off, selected
                                                    • subtitles off, selected
                                                    • en (Main), selected

                                                    如何使用 Invitrogen iBright 成像系统导出图片

                                                    剥离并在印迹膜上重新检测另一个蛋白靶点

                                                    Video Player is loading.
                                                    Current Time 0:00
                                                    Duration 1:54
                                                    Loaded: 0%
                                                    Stream Type LIVE
                                                    Remaining Time 1:54
                                                     
                                                    1x
                                                      • Chapters
                                                      • descriptions off, selected
                                                      • subtitles off, selected
                                                      • en (Main), selected

                                                      如何剥离抗体并重新检测wb印迹膜

                                                      仅供科研使用,不可用于诊断目的。