Krios 5 Cryo-TEM Applications

3D visualization of protein structures with cryo-electron microscopy

The 300 kV Thermo Scientific Krios 5 Cryo-Transmission Electron Microscope (Cryo-TEM) is engineered for the 3D visualization of proteins and molecular machines, as well as their localization and dynamics within the architecture of the cell, enabling you to obtain critical biological insights.

 

Cryo-EM technology can be applied across a variety of research areas, including in facilities with certain biosafety level requirements (e.g., BSL-3). Watch the video below to see how the Krios Cryo-TEM can be used in infectious disease research.

Exploring structure-function relationships with cryo-ET

Cryo-electron tomography eBook

Cryo-EM research: case studies and eBooks

See the examples below to learn how scientists have leveraged the Krios Cryo-TEM to answer challenging questions across the life sciences, from virology and cancer research, to drug discovery, plant biology, and more. 

Gaining insights into neuronal structure and function

Preview of cryo EM in neuroscience eBook

Untangling neurodegeneration with cryo-EM

Preview of cryo EM eBook on neurodegeneration

Understanding the complexity of cancer with cryo-EM

Preview of cryo EM cancer research eBook

Cryo-EM trends in drug discovery

Preview of cryo EM drug discovery eBook

Structural virology with cryo-EM

Preview of structural virology with cryo EM eBook

Novel insights into plant biology

Preview of cryo EM plant biology eBook

Atomic-resolution single particle analysis

Using a Krios Cryo-TEM equipped with a Thermo Scientific Selectris X Imaging Filter, structural biologists achieved record-breaking resolution results, featured on the cover of Nature and in other recent articles from Science and Nature. Scientists from the Medical Research Council Laboratory of Molecular Biology in Cambridge, UK, obtained a 1.2 Å resolution structure of the iron-storing protein apoferritin. They also achieved a 1.7 Å resolution for a GABA membrane protein, with even sharper resolution in key parts of the protein. In addition to the leap in resolution, Selectris Filters can also significantly impact productivity; with a better filter and camera, you can achieve a specific resolution with much less data.

Human respiratory syncytial virus F variant

Structure of human respiratory syncytial virus (RSV) F variant (construct pXCS847A) as described by Che et al. (PDB ID: 7UJA). The very first vaccine by GlaxoSmithKline (GSK) was approved by the US Food and Drug Administration in 2023.

Molecular structure of human respiratory syncytial virus RSV

Janus kinase dimer

Structure of active Janus kinase (JAK) dimer complexed with cytokine receptor intracellular domain as described by Glassman et al. (PDB ID: 7T6F). JAK is an essential component of cytokine signaling. Mutations to this kinase have led to immunodeficiency and myeloproliferative disorders.

Cryo-EM structure of janus kinase

SARS-CoV-2 Spike protein

Structural biology with cryo-EM has contributed to our understanding of SARS-CoV-2 since the beginning of the COVID-19 pandemic, providing critical information on the viral spike protein. Structure as described by Zhang et al. (PDB ID: 7KRQ). 

Cryo-EM structure of SARS-CoV-2 spike protein

TMEM106B filaments

Alzheimer’s disease associated TMEM106B filaments as described by Chang et al. (PDB ID: 7U11). They showed that a previously unsolved amyloid fibril is composed of TMEM106B, a transmembrane lysosomal/endosomal protein.

Cryo-EM structure of TMEM106B filaments with Fold 1 from Alzheimer’s disease

Cryo-electron tomography of cells

The Krios 5 Cryo-TEM offers excellent cryo-electron tomography (cryo-ET) capabilities, providing nanometer-scale 3D imaging of a cell’s interior as well as visualization of protein complexes within their physiological environment. Using the Krios Cryo-TEM, tomography data can be obtained from whole bacterial cells or lamellae prepared with Thermo Scientific Aquilos, Arctis or Hydra Bio Focused Ion Beam (Cryo-FIB) instruments.

Excitatory synapse of hippocampal neuron

Post-synaptic structure-like PSD filaments and glutamate receptors are clearly depicted as well as pre-synaptic structures such as vesicles or adhesion proteins.  Courtesy of Guoqiang Bi and Hong Zhou of UCLA.

3D visualization of a neuron excitatory synapse obtained with cryo electron tomography

Hear from scientists using the Krios Cryo-TEM

For Research Use Only. Not for use in diagnostic procedures.